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The map label for this gene is cheB3

Identifier: 34498891

GI number: 34498891

Start: 3732220

End: 3733299

Strand: Reverse

Name: cheB3

Synonym: CV_3436

Alternate gene names: 34498891

Gene position: 3733299-3732220 (Counterclockwise)

Preceding gene: 34498892

Following gene: 34498890

Centisome position: 78.58

GC content: 67.41

Gene sequence:

>1080_bases
ATGAGCGCGGGAACGGGGCGGAGAATCCGGGTGGTGGTGGTGGACGATTCGGCGCTGGTGCGCAGCCTGCTGACGGCCAT
CGTCAACGAGGCGCCGGACATGGAGGTGGTGGCGACGGCGTCCGATCCCATCATCGCCCGGGAAAAGATACGCGAAACCA
GCCCGGACGTGGTGACGCTGGATGTGGAAATGCCGCGGATGGACGGGCTGGAGTTCCTGCGGCGCCTGATGAGGCTGAGG
CCGACGCCGGTGTTGATGATCTCCTCGCTGACCGATGCCGGTTCGGAAACCACGCTGGCCGCGCTCGAGCTGGGCGCGGT
GGATTTCATCCACAAGCCGGCCGACGACATCGCGCGGCACATGCAGGAGTACGCGGAGGAGATACGGGAAAAGCTCAGGG
TCACGGCCGAAGCCAGGCTCAGGCAGCCGAACCGTTTCCGCCAGCCGCCGCCGGCGCGCAAGACGGGCCCAGGCGCGCCG
TTGAGGAAGAACGCGCTGGTCTTCGTCGGCGCGTCCACCGGCGGCACCGAGGCGATCAAGGACTTCCTGATGGGCATGCC
GGCCGACGGGCCGCCCATCCTGATCGTGCAGCACATGCCGGAGCATTTCACTTTCACTTTCGCCGAGCGCCTGAACAAGC
TGTGCCCGATGCGGGTGAAGGAGGCGGAGGACGGTGAGGCCGCGCTGGCCGGCGTTGCCTACATCGCGCCGGGGCACTCG
CACATGCGGGTGGCGCCCGCGGGGGGCGGCGGCTTCCGGATCGCCCTGGATCAAGAGGAGCCGGTCAACCGCCATCGGCC
GGCGGTGGATCCGTTGTTCGATTCCGCGGCCAGGCATCTGGGCAAGAACGCGATCGGCGTGATCCTGACCGGCATGGGCA
AGGACGGGGCGGAAGGGCTGTTGCGGATGCGCCATGCGGGCGCCGTCACCTTCGGCCAGGATGAGGCCAGCTGCGTGGTG
TACGGCATGCCGCGGGAAGCGTTCAGGATAGGCGCGGTGGCGCATGTCCTGCCATTGAACAGACTGGCGGAGAAAGTGGT
CGAGTGCCTGCAGAGCCAAGCCGAAAGGGCGATAACATGA

Upstream 100 bases:

>100_bases
TCGGACATTCGGAAAACATCGTCCACCTGACCGACGCCTTCATCGGCTGCGGCAGGACCACCTACCGGCTCGCGCATCCG
GACCGCGGAGAGGGGGGCGG

Downstream 100 bases:

>100_bases
ATCATGCCGAAGGGATGAACAGCCACCAGTACTACGACAAGCACTTCCAGATCACGGCGGTCAAGGTATTTCCGGGCGAG
TTTCACGCCACCAACCAGTC

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSAGTGRRIRVVVVDDSALVRSLLTAIVNEAPDMEVVATASDPIIAREKIRETSPDVVTLDVEMPRMDGLEFLRRLMRLR
PTPVLMISSLTDAGSETTLAALELGAVDFIHKPADDIARHMQEYAEEIREKLRVTAEARLRQPNRFRQPPPARKTGPGAP
LRKNALVFVGASTGGTEAIKDFLMGMPADGPPILIVQHMPEHFTFTFAERLNKLCPMRVKEAEDGEAALAGVAYIAPGHS
HMRVAPAGGGGFRIALDQEEPVNRHRPAVDPLFDSAARHLGKNAIGVILTGMGKDGAEGLLRMRHAGAVTFGQDEASCVV
YGMPREAFRIGAVAHVLPLNRLAEKVVECLQSQAERAIT

Sequences:

>Translated_359_residues
MSAGTGRRIRVVVVDDSALVRSLLTAIVNEAPDMEVVATASDPIIAREKIRETSPDVVTLDVEMPRMDGLEFLRRLMRLR
PTPVLMISSLTDAGSETTLAALELGAVDFIHKPADDIARHMQEYAEEIREKLRVTAEARLRQPNRFRQPPPARKTGPGAP
LRKNALVFVGASTGGTEAIKDFLMGMPADGPPILIVQHMPEHFTFTFAERLNKLCPMRVKEAEDGEAALAGVAYIAPGHS
HMRVAPAGGGGFRIALDQEEPVNRHRPAVDPLFDSAARHLGKNAIGVILTGMGKDGAEGLLRMRHAGAVTFGQDEASCVV
YGMPREAFRIGAVAHVLPLNRLAEKVVECLQSQAERAIT
>Mature_358_residues
SAGTGRRIRVVVVDDSALVRSLLTAIVNEAPDMEVVATASDPIIAREKIRETSPDVVTLDVEMPRMDGLEFLRRLMRLRP
TPVLMISSLTDAGSETTLAALELGAVDFIHKPADDIARHMQEYAEEIREKLRVTAEARLRQPNRFRQPPPARKTGPGAPL
RKNALVFVGASTGGTEAIKDFLMGMPADGPPILIVQHMPEHFTFTFAERLNKLCPMRVKEAEDGEAALAGVAYIAPGHSH
MRVAPAGGGGFRIALDQEEPVNRHRPAVDPLFDSAARHLGKNAIGVILTGMGKDGAEGLLRMRHAGAVTFGQDEASCVVY
GMPREAFRIGAVAHVLPLNRLAEKVVECLQSQAERAIT

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=340, Percent_Identity=55, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB3_CHRVO (Q7NSI8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903106.1
- ProteinModelPortal:   Q7NSI8
- SMR:   Q7NSI8
- GeneID:   2548249
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3436
- NMPDR:   fig|243365.1.peg.3436
- HOGENOM:   HBG705324
- OMA:   FVTKPKL
- PhylomeDB:   Q7NSI8
- BioCyc:   CVIO243365:CV_3436-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38920; Mature: 38788

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 172-172 ACT_SITE 198-198 ACT_SITE 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGTGRRIRVVVVDDSALVRSLLTAIVNEAPDMEVVATASDPIIAREKIRETSPDVVTL
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEE
DVEMPRMDGLEFLRRLMRLRPTPVLMISSLTDAGSETTLAALELGAVDFIHKPADDIARH
EECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
MQEYAEEIREKLRVTAEARLRQPNRFRQPPPARKTGPGAPLRKNALVFVGASTGGTEAIK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHH
DFLMGMPADGPPILIVQHMPEHFTFTFAERLNKLCPMRVKEAEDGEAALAGVAYIAPGHS
HHHHCCCCCCCCEEEEECCCCHHEEHHHHHHHHHCCCCCCCCCCCCHHHHEEEEECCCCC
HMRVAPAGGGGFRIALDQEEPVNRHRPAVDPLFDSAARHLGKNAIGVILTGMGKDGAEGL
CEEEEECCCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
LRMRHAGAVTFGQDEASCVVYGMPREAFRIGAVAHVLPLNRLAEKVVECLQSQAERAIT
HHHHHCCCEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAGTGRRIRVVVVDDSALVRSLLTAIVNEAPDMEVVATASDPIIAREKIRETSPDVVTL
CCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEE
DVEMPRMDGLEFLRRLMRLRPTPVLMISSLTDAGSETTLAALELGAVDFIHKPADDIARH
EECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
MQEYAEEIREKLRVTAEARLRQPNRFRQPPPARKTGPGAPLRKNALVFVGASTGGTEAIK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHH
DFLMGMPADGPPILIVQHMPEHFTFTFAERLNKLCPMRVKEAEDGEAALAGVAYIAPGHS
HHHHCCCCCCCCEEEEECCCCHHEEHHHHHHHHHCCCCCCCCCCCCHHHHEEEEECCCCC
HMRVAPAGGGGFRIALDQEEPVNRHRPAVDPLFDSAARHLGKNAIGVILTGMGKDGAEGL
CEEEEECCCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
LRMRHAGAVTFGQDEASCVVYGMPREAFRIGAVAHVLPLNRLAEKVVECLQSQAERAIT
HHHHHCCCEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782