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The map label for this gene is minD [H]

Identifier: 34498831

GI number: 34498831

Start: 3671422

End: 3672234

Strand: Direct

Name: minD [H]

Synonym: CV_3376

Alternate gene names: 34498831

Gene position: 3671422-3672234 (Clockwise)

Preceding gene: 34498830

Following gene: 34498832

Centisome position: 77.28

GC content: 63.59

Gene sequence:

>813_bases
GTGGCAAAAATCATCGTTGTGACATCCGGCAAGGGTGGCGTAGGCAAAACCACCACCAGCGCGAGCTTCGCCTCCGGCCT
GGCACTGCGCGGCCACAAAACTGCCGTGATCGACTTCGACGTCGGCCTGCGCAACCTGGACCTGATCATGGGCTGCGAAC
GCCGCGTGGTGTACGACCTGATCAACGTGGTCAACGGCGAAGCCAGCCTGAACCAGGCGCTGATCAAGGACAAGCACTGC
GACAATCTCTACATCCTCCCGGCCTCCCAAACCCGCGACAAGGACGCGCTGACGATAGAAGGCGTGGAGAAGGTGCTGAA
GGACCTGGAAGAAGCCGGCTTCGACTACATCGTCTGCGACTCACCCGCCGGCATCGAGAAGGGCGCGCTGATGTCGCTCT
ACTTCGCCGACGAGGCGCTGATCGTCACCAATCCGGAAGTGTCGTCGGTGCGCGACTCCGACCGCATCCTGGGCATCCTG
TCGTCCAAGTCCAAGCGCGCCGAGGAGAACCGGGAGCCGGTGAAGGAGCACCTGCTGATCACCCGCTACGCGCCGGGCCG
CGTGGACAAGGGCGAAATGCTTTCGGTGGACGACGTGAAGGAAATCCTGCGCGTGCCGCTGATCGGCGTCATCCCCGAAT
CCCAGAGCGTGCTGCAGGCATCCAACTCCGGCACCCCCGCCATCCACCTGAAGGGCAGCGACGTGGCCGAAGCCTACGGC
GACGTGATCTCGCGCTTCCTGGGCGAAGAACGCCCGATGCGCTTCCTGGACGCCCCCAAGGTGGGCATCCTCAAGCGCCT
GTTCGGAGGTTAA

Upstream 100 bases:

>100_bases
TGCCGGACAGCATTCTGGGCAAGCCCACCCAAATCTATCTTGAGAACGAGCGGCTGGTGATGACCGCGCTCGGCGAGTAA
CACATCCAAAGGAAAGCCCC

Downstream 100 bases:

>100_bases
GCCATGTCACTGATTGATATGATTTTTGGCAAGCGCCAGAAATCCGCCGCCATCGCCCGCGAACGTCTGCAGATCATCCT
GGCGCACGAGCGCAACGGCC

Product: septum site-determining protein minD

Products: NA

Alternate protein names: Cell division inhibitor minD [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAKIIVVTSGKGGVGKTTTSASFASGLALRGHKTAVIDFDVGLRNLDLIMGCERRVVYDLINVVNGEASLNQALIKDKHC
DNLYILPASQTRDKDALTIEGVEKVLKDLEEAGFDYIVCDSPAGIEKGALMSLYFADEALIVTNPEVSSVRDSDRILGIL
SSKSKRAEENREPVKEHLLITRYAPGRVDKGEMLSVDDVKEILRVPLIGVIPESQSVLQASNSGTPAIHLKGSDVAEAYG
DVISRFLGEERPMRFLDAPKVGILKRLFGG

Sequences:

>Translated_270_residues
MAKIIVVTSGKGGVGKTTTSASFASGLALRGHKTAVIDFDVGLRNLDLIMGCERRVVYDLINVVNGEASLNQALIKDKHC
DNLYILPASQTRDKDALTIEGVEKVLKDLEEAGFDYIVCDSPAGIEKGALMSLYFADEALIVTNPEVSSVRDSDRILGIL
SSKSKRAEENREPVKEHLLITRYAPGRVDKGEMLSVDDVKEILRVPLIGVIPESQSVLQASNSGTPAIHLKGSDVAEAYG
DVISRFLGEERPMRFLDAPKVGILKRLFGG
>Mature_269_residues
AKIIVVTSGKGGVGKTTTSASFASGLALRGHKTAVIDFDVGLRNLDLIMGCERRVVYDLINVVNGEASLNQALIKDKHCD
NLYILPASQTRDKDALTIEGVEKVLKDLEEAGFDYIVCDSPAGIEKGALMSLYFADEALIVTNPEVSSVRDSDRILGILS
SKSKRAEENREPVKEHLLITRYAPGRVDKGEMLSVDDVKEILRVPLIGVIPESQSVLQASNSGTPAIHLKGSDVAEAYGD
VISRFLGEERPMRFLDAPKVGILKRLFGG

Specific function: ATPase required for the correct placement of the division site. Cell division inhibitors minC and minD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to desta

COG id: COG2894

COG function: function code D; Septum formation inhibitor-activating ATPase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the parA family. MinD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787423, Length=270, Percent_Identity=72.5925925925926, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR010223 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: NA

Molecular weight: Translated: 29224; Mature: 29093

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIIVVTSGKGGVGKTTTSASFASGLALRGHKTAVIDFDVGLRNLDLIMGCERRVVYDL
CEEEEEEECCCCCCCCCCCCHHHHCCEEECCCCEEEEEECCCCCHHHHHHCCCHHHHHHH
INVVNGEASLNQALIKDKHCDNLYILPASQTRDKDALTIEGVEKVLKDLEEAGFDYIVCD
HHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEC
SPAGIEKGALMSLYFADEALIVTNPEVSSVRDSDRILGILSSKSKRAEENREPVKEHLLI
CCCCCCCCCEEEEEECCCEEEEECCCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCEEE
TRYAPGRVDKGEMLSVDDVKEILRVPLIGVIPESQSVLQASNSGTPAIHLKGSDVAEAYG
EECCCCCCCCCCEECHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCEEEECCCHHHHHHH
DVISRFLGEERPMRFLDAPKVGILKRLFGG
HHHHHHHCCCCCCHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKIIVVTSGKGGVGKTTTSASFASGLALRGHKTAVIDFDVGLRNLDLIMGCERRVVYDL
EEEEEEECCCCCCCCCCCCHHHHCCEEECCCCEEEEEECCCCCHHHHHHCCCHHHHHHH
INVVNGEASLNQALIKDKHCDNLYILPASQTRDKDALTIEGVEKVLKDLEEAGFDYIVCD
HHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEC
SPAGIEKGALMSLYFADEALIVTNPEVSSVRDSDRILGILSSKSKRAEENREPVKEHLLI
CCCCCCCCCEEEEEECCCEEEEECCCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCEEE
TRYAPGRVDKGEMLSVDDVKEILRVPLIGVIPESQSVLQASNSGTPAIHLKGSDVAEAYG
EECCCCCCCCCCEECHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCEEEECCCHHHHHHH
DVISRFLGEERPMRFLDAPKVGILKRLFGG
HHHHHHHCCCCCCHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]