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The map label for this gene is ptsN [H]

Identifier: 34498789

GI number: 34498789

Start: 3632029

End: 3632490

Strand: Direct

Name: ptsN [H]

Synonym: CV_3334

Alternate gene names: 34498789

Gene position: 3632029-3632490 (Clockwise)

Preceding gene: 34498788

Following gene: 161594050

Centisome position: 76.45

GC content: 65.58

Gene sequence:

>462_bases
ATGAATCTCATCGGCAAGATCCTGACCCCGGACCATATCCTGCTGGACCTGGACGTCGCCAGCAAGAAACGGGTGTTCGA
GCAGGTGGGCCTGCTGGTGGAAAACACCCGCGGCATCGCCCGCAGCGAGATTTTCGACAGCCTGTTCAGCCGTGAAAAAC
TGGGCTCCACCGGACTCGGCCAGGGCGTGGCCATCCCCCATGGCCGCGCGCGCGGCCTGAAGGAAGCCACCGGCGTGTTC
ATCCGCCTGAAGGCGCCCATCCCCTTCGACGCCCCGGACGGCAAGCCGGTGCAATGCCTGTTCGTGCTGCTGGTGCCCGA
GCACGCCACCGACCTGCATCTGCAGGTGCTGTCGGAGCTGGCGCAGATGTTCTCCAGCCGCCAGATGCGCGAGCAGATGC
TGGGCGTGGACGCGCGCGACGACCTGTACCAGCTGCTTTGCGAATGGACCCCCCATGCCTAG

Upstream 100 bases:

>100_bases
GCCTGGCGGTTCCCGTTTTTCTTTGCGCTTGCCGGTTTTCAGTACTATCAAGGCAACGTAGAATCCACAGTTTCCCCAAA
AGCCGCATGCATAATCCAAC

Downstream 100 bases:

>100_bases
CATCACCGTGCGCCGGCTGTATCAGGAAAACCAGCAGAAACTGAATCTCACCTGGGTCGCCGGCACCGGCGGCTCCGACA
ATGTGATTGGCAACGACGAC

Product: PTS system fructose-specific transporter subunit IIABC

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MNLIGKILTPDHILLDLDVASKKRVFEQVGLLVENTRGIARSEIFDSLFSREKLGSTGLGQGVAIPHGRARGLKEATGVF
IRLKAPIPFDAPDGKPVQCLFVLLVPEHATDLHLQVLSELAQMFSSRQMREQMLGVDARDDLYQLLCEWTPHA

Sequences:

>Translated_153_residues
MNLIGKILTPDHILLDLDVASKKRVFEQVGLLVENTRGIARSEIFDSLFSREKLGSTGLGQGVAIPHGRARGLKEATGVF
IRLKAPIPFDAPDGKPVQCLFVLLVPEHATDLHLQVLSELAQMFSSRQMREQMLGVDARDDLYQLLCEWTPHA
>Mature_153_residues
MNLIGKILTPDHILLDLDVASKKRVFEQVGLLVENTRGIARSEIFDSLFSREKLGSTGLGQGVAIPHGRARGLKEATGVF
IRLKAPIPFDAPDGKPVQCLFVLLVPEHATDLHLQVLSELAQMFSSRQMREQMLGVDARDDLYQLLCEWTPHA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=134, Percent_Identity=38.8059701492537, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1786951, Length=111, Percent_Identity=32.4324324324324, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16985; Mature: 16985

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIGKILTPDHILLDLDVASKKRVFEQVGLLVENTRGIARSEIFDSLFSREKLGSTGLG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
QGVAIPHGRARGLKEATGVFIRLKAPIPFDAPDGKPVQCLFVLLVPEHATDLHLQVLSEL
CCCCCCCCHHCCCHHHCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
AQMFSSRQMREQMLGVDARDDLYQLLCEWTPHA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLIGKILTPDHILLDLDVASKKRVFEQVGLLVENTRGIARSEIFDSLFSREKLGSTGLG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
QGVAIPHGRARGLKEATGVFIRLKAPIPFDAPDGKPVQCLFVLLVPEHATDLHLQVLSEL
CCCCCCCCHHCCCHHHCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
AQMFSSRQMREQMLGVDARDDLYQLLCEWTPHA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]