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The map label for this gene is alkA [H]

Identifier: 34498650

GI number: 34498650

Start: 3479397

End: 3480290

Strand: Reverse

Name: alkA [H]

Synonym: CV_3195

Alternate gene names: 34498650

Gene position: 3480290-3479397 (Counterclockwise)

Preceding gene: 34498651

Following gene: 34498649

Centisome position: 73.25

GC content: 73.27

Gene sequence:

>894_bases
ATGTCCGTCATCGATTGCGATATCGCGCTGCCGGCCGGCTTCCGGCCGCAGGACATCCTGGCCTTCCACCGTCGCGACGG
CGAGCGCCTCGCCGAGCAGGTGGACGACGACGGCCTGAGAAAGGGCCTGTTGTGGCAAGGCCTGCCCGCCTGCCTAGACT
TGCGCTTCCGGCCCGGCTGGGCCGTCCTCAGGCTGGATGTCGACGGCGGGGCGGCAGAGGCCCGGGCGCTGGAGGACATC
GGCCGGCGCATGCTGGGCCTGAACCAGCCGGTGGAGGCGTTCGAGCGCCGATACCGCGACCATCCCCAACTGGGCGGCCT
GATCGCCGCGCGCGCCGGCCTCAGGGTGCCGCAGACGGCCACGCCGTTCGAGGCGCTGGCCTGGGCGATCACCGGCCAGC
AGATCAGCGTGGCGGCGGCGGTGACGATACGGCGGCGGCTGCTGCAATTGTGCGATTGCCGCCATTCTTCCGGCCTGCTG
TGCCACCCGGACGCGCCGCGGCTGGCGGCGCTGACGGCGGATCAACTGGGCGAGGCCGGCTTTTCCCGCGCCAAGAGCCG
GGCCTTGCTGGCGCTGAGCCAGGGCGCGGCGAGCGGCGCGCTGCCGCTGGATGGCTGGCTGGACGGCGCGCCGGCGGAGG
AGATCGCCGAGCGGCTGCTGGCGGTGCCGGGCATCGGGCCATGGACGGTCAGCTACGCGCTGTTGCGCGGCTACGGCTGG
CTGGACGGCTCGCTGCACGGCGACGTGGCGGTGCGCAAGGCGCTGGGCATGGCGCTGGGCATGGCCGACAAGCCCGACCA
GAAGCAGGCGCAGCAGTGGCTGGACGCGTTCTCGCCGTGGCGGGCGCTGGTGGCCGCCCACCTGTGGGCATCGCTGCAGG
CGGGGGGATTCTGA

Upstream 100 bases:

>100_bases
CCGGTTGCGCTTTCATGGCGTGCTGCCGGTCCAGCCGGGCATTCACCCGCTGCTCGGCGGCCGGCGTCTCAACCTCACTT
TCCGCAAGGCCTTTTGATCC

Downstream 100 bases:

>100_bases
CCGCTCGCGCGATCCGCCGCGCTTTGTCGAGGCGATATCGCAAACGCCATCCGAATGGCTATCTTCGCGGGCAAGTTCCG
CTTTTCTCGCCGCGCTGTCT

Product: DNA-3-methyladenine glycosidase II

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase II, inducible; TAG II; DNA-3-methyladenine glycosidase II; DNA-3-methyladenine glycosylase II [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSVIDCDIALPAGFRPQDILAFHRRDGERLAEQVDDDGLRKGLLWQGLPACLDLRFRPGWAVLRLDVDGGAAEARALEDI
GRRMLGLNQPVEAFERRYRDHPQLGGLIAARAGLRVPQTATPFEALAWAITGQQISVAAAVTIRRRLLQLCDCRHSSGLL
CHPDAPRLAALTADQLGEAGFSRAKSRALLALSQGAASGALPLDGWLDGAPAEEIAERLLAVPGIGPWTVSYALLRGYGW
LDGSLHGDVAVRKALGMALGMADKPDQKQAQQWLDAFSPWRALVAAHLWASLQAGGF

Sequences:

>Translated_297_residues
MSVIDCDIALPAGFRPQDILAFHRRDGERLAEQVDDDGLRKGLLWQGLPACLDLRFRPGWAVLRLDVDGGAAEARALEDI
GRRMLGLNQPVEAFERRYRDHPQLGGLIAARAGLRVPQTATPFEALAWAITGQQISVAAAVTIRRRLLQLCDCRHSSGLL
CHPDAPRLAALTADQLGEAGFSRAKSRALLALSQGAASGALPLDGWLDGAPAEEIAERLLAVPGIGPWTVSYALLRGYGW
LDGSLHGDVAVRKALGMALGMADKPDQKQAQQWLDAFSPWRALVAAHLWASLQAGGF
>Mature_296_residues
SVIDCDIALPAGFRPQDILAFHRRDGERLAEQVDDDGLRKGLLWQGLPACLDLRFRPGWAVLRLDVDGGAAEARALEDIG
RRMLGLNQPVEAFERRYRDHPQLGGLIAARAGLRVPQTATPFEALAWAITGQQISVAAAVTIRRRLLQLCDCRHSSGLLC
HPDAPRLAALTADQLGEAGFSRAKSRALLALSQGAASGALPLDGWLDGAPAEEIAERLLAVPGIGPWTVSYALLRGYGWL
DGSLHGDVAVRKALGMALGMADKPDQKQAQQWLDAFSPWRALVAAHLWASLQAGGF

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=235, Percent_Identity=32.3404255319149, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 31984; Mature: 31853

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIDCDIALPAGFRPQDILAFHRRDGERLAEQVDDDGLRKGLLWQGLPACLDLRFRPGW
CCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCHHHHHHCHHHCCCHHHHEEEECCCE
AVLRLDVDGGAAEARALEDIGRRMLGLNQPVEAFERRYRDHPQLGGLIAARAGLRVPQTA
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
TPFEALAWAITGQQISVAAAVTIRRRLLQLCDCRHSSGLLCHPDAPRLAALTADQLGEAG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHH
FSRAKSRALLALSQGAASGALPLDGWLDGAPAEEIAERLLAVPGIGPWTVSYALLRGYGW
HHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
LDGSLHGDVAVRKALGMALGMADKPDQKQAQQWLDAFSPWRALVAAHLWASLQAGGF
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVIDCDIALPAGFRPQDILAFHRRDGERLAEQVDDDGLRKGLLWQGLPACLDLRFRPGW
CEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCHHHHHHCHHHCCCHHHHEEEECCCE
AVLRLDVDGGAAEARALEDIGRRMLGLNQPVEAFERRYRDHPQLGGLIAARAGLRVPQTA
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
TPFEALAWAITGQQISVAAAVTIRRRLLQLCDCRHSSGLLCHPDAPRLAALTADQLGEAG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHH
FSRAKSRALLALSQGAASGALPLDGWLDGAPAEEIAERLLAVPGIGPWTVSYALLRGYGW
HHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
LDGSLHGDVAVRKALGMALGMADKPDQKQAQQWLDAFSPWRALVAAHLWASLQAGGF
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6094528; 9097040; 9278503; 6389535; 3529081; 8706135; 8706136 [H]