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The map label for this gene is mesJ

Identifier: 34498644

GI number: 34498644

Start: 3470335

End: 3471642

Strand: Reverse

Name: mesJ

Synonym: CV_3189

Alternate gene names: 34498644

Gene position: 3471642-3470335 (Counterclockwise)

Preceding gene: 34498645

Following gene: 34498642

Centisome position: 73.07

GC content: 70.11

Gene sequence:

>1308_bases
CTGGCGGATAGCCTGATCGAGCATTGGCCGGACGAGTTGTCCGGCCTTTTCGCTTTTGAGGTCGGGTTTTCCGGCGGACT
CGACTCGGTCGCCTTGCTGTCGCTGCTGTGCGAGGCGCGCGCGCGCCGGCCGGAGATCGGCTTGTCCGCGGTGCACGTTC
ATCACGGCTTGAGTCCAAACGCCGATGCCTGGGCCGCGCATTGCCAGGCGCTGTGCGACTCCCTGCGGGTGCCTTTGCGC
ATCGAACGGGTGCACGTCCGCGCCGGCGGCGGCGAAAGCGTCGAGGCGGCGGCGCGCGAGGCGCGCTACCAGGTTTACCG
GCGCTCCGCATCCGAGGTGATCGCGCTGGCCCACCATCAGGACGATCAAGCCGAAACCATCCTGCTGCAACTGTTGCGCG
GCGGCGGCGCGCGGGCGCTGGCGGCGATGCCGGCATTGCGCGAACTGGCGCCGGGCAAGCTGCTGTGGCGGCCGTTGCTG
GAGATCCCCCGCGAGCGGCTGGAGGCTTACGTCAAGGCGCGCGGGTTTGCCTGGGTGGACGACGAAAGCAATCTGGACAC
CCGCTACCGTCGCAACCTGTTGCGGCACGACATTCTGCCTCGGCTGGAGCGGGCGCTTCCGCATTACCGCAGCCACCTGG
CGCGCGCGGCGATGCTGCAGGCGGATGCCGCCGCCATCCTGGACGAAGTCGCGCGGGAAGACCTGCAAGCCTGCCGAACG
GAAAACGGATTGGACTGCGGGAGCGTGCTGGCGCTGTCCGCGCCGCGCCGGCGCCAGACCCTGCTTGCCTGGCTGGATGC
GCTGGGTTGGCCCGCGCCGGAGCCGGGCGCGCTGTTGGAGTTTTTGCGGCAGGCGGAATACGCGGAGCGGGGCGCCAGCC
CGGTTCTGAGACTGCGGCAGGGGTGTCTGTTGCGTTTTGCCGATACTTTGCAGGCCTGGCCTAAAGCACAGTCAGAGCCG
CCGGCCTCCACTGCTCTGCTGTGGCGCGGAGGCGACGCTCAGTCGCTGTCCGAGTGGGGCGGCGAACTGGCCTGGGAGCG
GCGAGACGCCGGCGTGCCAGCGGCCTTGCTGGAAAGCGGCGCCTATCTGGCGCCGCGGCGAGGCGGAGAAAAACTGTCGG
CTCGGGTTGGGCGGCGCGAAGTCAAGGACCTGCTGCGGGAGGCTGGGGTGCCGCCGTTGCTGCGTCGCCGCTGGCCGCTG
CTGTATGGCGCGGATGGCGAATTGCTGGCGGTGCCGGGCATCGCGGTCTCCTACCGCGCGGCGGCGGATGGCGGCTGGTG
GCCGTTGTGGCGGCCTGCCCGTCCTTAG

Upstream 100 bases:

>100_bases
CAGCTGAAAGAAGTGCAGTCCAAGCCCATGGATGCGCTGCTGAAAGAACGTTTTGATCGCCTGATGAGCTATGGCCGCTT
CAAGGAAGACGCCGCCTGAG

Downstream 100 bases:

>100_bases
AAACGCCGCCGTTGGCGGACCGTCGGCGGCGGATGAGGGTCTTCCCCAGAACTCAGGCCGCTTGACGCGATGGGCGTTCC
CCAAGTCCCCGGATCGCCGT

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MADSLIEHWPDELSGLFAFEVGFSGGLDSVALLSLLCEARARRPEIGLSAVHVHHGLSPNADAWAAHCQALCDSLRVPLR
IERVHVRAGGGESVEAAAREARYQVYRRSASEVIALAHHQDDQAETILLQLLRGGGARALAAMPALRELAPGKLLWRPLL
EIPRERLEAYVKARGFAWVDDESNLDTRYRRNLLRHDILPRLERALPHYRSHLARAAMLQADAAAILDEVAREDLQACRT
ENGLDCGSVLALSAPRRRQTLLAWLDALGWPAPEPGALLEFLRQAEYAERGASPVLRLRQGCLLRFADTLQAWPKAQSEP
PASTALLWRGGDAQSLSEWGGELAWERRDAGVPAALLESGAYLAPRRGGEKLSARVGRREVKDLLREAGVPPLLRRRWPL
LYGADGELLAVPGIAVSYRAAADGGWWPLWRPARP

Sequences:

>Translated_435_residues
MADSLIEHWPDELSGLFAFEVGFSGGLDSVALLSLLCEARARRPEIGLSAVHVHHGLSPNADAWAAHCQALCDSLRVPLR
IERVHVRAGGGESVEAAAREARYQVYRRSASEVIALAHHQDDQAETILLQLLRGGGARALAAMPALRELAPGKLLWRPLL
EIPRERLEAYVKARGFAWVDDESNLDTRYRRNLLRHDILPRLERALPHYRSHLARAAMLQADAAAILDEVAREDLQACRT
ENGLDCGSVLALSAPRRRQTLLAWLDALGWPAPEPGALLEFLRQAEYAERGASPVLRLRQGCLLRFADTLQAWPKAQSEP
PASTALLWRGGDAQSLSEWGGELAWERRDAGVPAALLESGAYLAPRRGGEKLSARVGRREVKDLLREAGVPPLLRRRWPL
LYGADGELLAVPGIAVSYRAAADGGWWPLWRPARP
>Mature_434_residues
ADSLIEHWPDELSGLFAFEVGFSGGLDSVALLSLLCEARARRPEIGLSAVHVHHGLSPNADAWAAHCQALCDSLRVPLRI
ERVHVRAGGGESVEAAAREARYQVYRRSASEVIALAHHQDDQAETILLQLLRGGGARALAAMPALRELAPGKLLWRPLLE
IPRERLEAYVKARGFAWVDDESNLDTRYRRNLLRHDILPRLERALPHYRSHLARAAMLQADAAAILDEVAREDLQACRTE
NGLDCGSVLALSAPRRRQTLLAWLDALGWPAPEPGALLEFLRQAEYAERGASPVLRLRQGCLLRFADTLQAWPKAQSEPP
ASTALLWRGGDAQSLSEWGGELAWERRDAGVPAALLESGAYLAPRRGGEKLSARVGRREVKDLLREAGVPPLLRRRWPLL
YGADGELLAVPGIAVSYRAAADGGWWPLWRPARP

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=419, Percent_Identity=37.4701670644391, Blast_Score=218, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_CHRVO (Q7NT72)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902859.1
- ProteinModelPortal:   Q7NT72
- SMR:   Q7NT72
- GeneID:   2548104
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3189
- NMPDR:   fig|243365.1.peg.3189
- HOGENOM:   HBG668702
- OMA:   INLTPWL
- PhylomeDB:   Q7NT72
- BioCyc:   CVIO243365:CV_3189-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 47947; Mature: 47816

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSLIEHWPDELSGLFAFEVGFSGGLDSVALLSLLCEARARRPEIGLSAVHVHHGLSPN
CCCCHHHHCCHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
ADAWAAHCQALCDSLRVPLRIERVHVRAGGGESVEAAAREARYQVYRRSASEVIALAHHQ
HHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DDQAETILLQLLRGGGARALAAMPALRELAPGKLLWRPLLEIPRERLEAYVKARGFAWVD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEC
DESNLDTRYRRNLLRHDILPRLERALPHYRSHLARAAMLQADAAAILDEVAREDLQACRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ENGLDCGSVLALSAPRRRQTLLAWLDALGWPAPEPGALLEFLRQAEYAERGASPVLRLRQ
CCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
GCLLRFADTLQAWPKAQSEPPASTALLWRGGDAQSLSEWGGELAWERRDAGVPAALLESG
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHEEHHHCCCCCHHHHHCC
AYLAPRRGGEKLSARVGRREVKDLLREAGVPPLLRRRWPLLYGADGELLAVPGIAVSYRA
CEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCCCCEEECCCCEEEEEE
AADGGWWPLWRPARP
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADSLIEHWPDELSGLFAFEVGFSGGLDSVALLSLLCEARARRPEIGLSAVHVHHGLSPN
CCCHHHHCCHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
ADAWAAHCQALCDSLRVPLRIERVHVRAGGGESVEAAAREARYQVYRRSASEVIALAHHQ
HHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DDQAETILLQLLRGGGARALAAMPALRELAPGKLLWRPLLEIPRERLEAYVKARGFAWVD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEC
DESNLDTRYRRNLLRHDILPRLERALPHYRSHLARAAMLQADAAAILDEVAREDLQACRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ENGLDCGSVLALSAPRRRQTLLAWLDALGWPAPEPGALLEFLRQAEYAERGASPVLRLRQ
CCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
GCLLRFADTLQAWPKAQSEPPASTALLWRGGDAQSLSEWGGELAWERRDAGVPAALLESG
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHEEHHHCCCCCHHHHHCC
AYLAPRRGGEKLSARVGRREVKDLLREAGVPPLLRRRWPLLYGADGELLAVPGIAVSYRA
CEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCCCCEEECCCCEEEEEE
AADGGWWPLWRPARP
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782