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The map label for this gene is fixL [H]

Identifier: 34498544

GI number: 34498544

Start: 3368966

End: 3370210

Strand: Reverse

Name: fixL [H]

Synonym: CV_3089

Alternate gene names: 34498544

Gene position: 3370210-3368966 (Counterclockwise)

Preceding gene: 34498545

Following gene: 34498539

Centisome position: 70.94

GC content: 64.26

Gene sequence:

>1245_bases
CTGTTGCTGACGCTGCTGCTGGCCGCGGTGGGGTTGATCTATTCTCAGCGCATGGCCCTGCGTAGGCAGCGCAGCCTGGC
GGACGAGGAGCTGTTTTTGCAGAGTGAACGGGCGTCGGTGACGCTGAATGCGATTCACGACGGCGTGCTGATCCTGAGGC
AGGACCGCAGCATCGAGATGGCGAATCCCATCGCGCTGAGGCTGGTGGAGATGCCGGCCGAGGAGGTGCTGGGTCAGCCT
TGCCATGAGGTGTTCCGCCTGCTGGGCGAGCTGGCTTCGGAGTTCGCCGAAGATCCGATAGCCGAATGCTTCCGCACCGG
CGCGCCGGTGGAGTTGCAGGAGAGGATGCAACTGACGACGGCGCGGGGCAAGGTGCATTTCGTCGAAGGCGGCATCGCGC
CGCTGCTGTCGCGCGATGGCGTGCTGATCGGCGTGGTGTGCGCGCTGCGGGACATGGGGCCGCTGCGTCAACGGACGGCG
GAAGCGCTGGAGGCCAGCGAGACGCGGGTCAAGGAGCACCTGGAGAAGCTGTCCCATGTGGCGCGCTTGCATACCATGGG
TGAAATGGCCTCGGGCATCGCGCATGAGCTCAATCAGCCGTTGACAGCCATTTCCAATTATTGCCAAGCCGCGATGCAGA
TGCTGGAAGGCGTGGACGACGCGCCGCCCCAGGTGCATTCGGCGCTGAAGCTGGCGGTGGCGCAGGCGGGCCGGGCCGGG
GAGATCATCAAGCGCTTGCGCGCGCTGGTCAGCAAGCGGGCGATAGAAACCCGGCTGATCGACATCAACCAGGTAGTCGG
CAATTGCATGTTCCTGGCCGAGTACGACTTGCGCGACCGGGGCATCGAAGTGGTGCAGCTGTTGTCCGGGTATTCGATCC
CGGTGATGGCGGACAGCATACAGCTGGAGCAGGTGGTGCTGAACCTGTTATCCAATGCGATGGATGCGCTGAAGGACGAG
CCGCCGATGAAGCGCCATGTGATCATCCATACGCGGCGCGAGGGGCGGAAAGGGATACTGGAGGTGCGCGACACCGGCCG
AGGCATTACGCCCGATCTGCTGGAGGTGTTGTTCCACCCGTTCTTTTCCACCAAGCCGCATGGCATGGGGCTTGGCTTGT
CGATCTGCCAGACCATCGTCGAGCAGTACGGCGGGCTGATCTCGGCGGAAAACATCCCGGCCAGCGGCGCCTGCTTCCGC
ATCGAGCTGCCGCTGGCTTTGCAGGCGGAGCGGCAGGTGAGCTGA

Upstream 100 bases:

>100_bases
GCGATTGTCCTTGAACAGCGAAGCCCAGCCATTTGTGCTTGGGGTGGCGCAGGACATCCGGATCAGCGATCTGGCCCTGC
TGCTGTTGCTGCTGCGCGAA

Downstream 100 bases:

>100_bases
CGGTCAGGGCGCCCGGGACAGGGCGCCTGGCTGGGCGCGTTGAGCGCGTTCCTCGCGCGGGGTGATGTTGAAATGGTTGC
GGTAGGTGCTGGAGAAATGC

Product: two-component system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MLLTLLLAAVGLIYSQRMALRRQRSLADEELFLQSERASVTLNAIHDGVLILRQDRSIEMANPIALRLVEMPAEEVLGQP
CHEVFRLLGELASEFAEDPIAECFRTGAPVELQERMQLTTARGKVHFVEGGIAPLLSRDGVLIGVVCALRDMGPLRQRTA
EALEASETRVKEHLEKLSHVARLHTMGEMASGIAHELNQPLTAISNYCQAAMQMLEGVDDAPPQVHSALKLAVAQAGRAG
EIIKRLRALVSKRAIETRLIDINQVVGNCMFLAEYDLRDRGIEVVQLLSGYSIPVMADSIQLEQVVLNLLSNAMDALKDE
PPMKRHVIIHTRREGRKGILEVRDTGRGITPDLLEVLFHPFFSTKPHGMGLGLSICQTIVEQYGGLISAENIPASGACFR
IELPLALQAERQVS

Sequences:

>Translated_414_residues
MLLTLLLAAVGLIYSQRMALRRQRSLADEELFLQSERASVTLNAIHDGVLILRQDRSIEMANPIALRLVEMPAEEVLGQP
CHEVFRLLGELASEFAEDPIAECFRTGAPVELQERMQLTTARGKVHFVEGGIAPLLSRDGVLIGVVCALRDMGPLRQRTA
EALEASETRVKEHLEKLSHVARLHTMGEMASGIAHELNQPLTAISNYCQAAMQMLEGVDDAPPQVHSALKLAVAQAGRAG
EIIKRLRALVSKRAIETRLIDINQVVGNCMFLAEYDLRDRGIEVVQLLSGYSIPVMADSIQLEQVVLNLLSNAMDALKDE
PPMKRHVIIHTRREGRKGILEVRDTGRGITPDLLEVLFHPFFSTKPHGMGLGLSICQTIVEQYGGLISAENIPASGACFR
IELPLALQAERQVS
>Mature_414_residues
MLLTLLLAAVGLIYSQRMALRRQRSLADEELFLQSERASVTLNAIHDGVLILRQDRSIEMANPIALRLVEMPAEEVLGQP
CHEVFRLLGELASEFAEDPIAECFRTGAPVELQERMQLTTARGKVHFVEGGIAPLLSRDGVLIGVVCALRDMGPLRQRTA
EALEASETRVKEHLEKLSHVARLHTMGEMASGIAHELNQPLTAISNYCQAAMQMLEGVDDAPPQVHSALKLAVAQAGRAG
EIIKRLRALVSKRAIETRLIDINQVVGNCMFLAEYDLRDRGIEVVQLLSGYSIPVMADSIQLEQVVLNLLSNAMDALKDE
PPMKRHVIIHTRREGRKGILEVRDTGRGITPDLLEVLFHPFFSTKPHGMGLGLSICQTIVEQYGGLISAENIPASGACFR
IELPLALQAERQVS

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=403, Percent_Identity=25.0620347394541, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=30, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790300, Length=385, Percent_Identity=26.4935064935065, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081816, Length=113, Percent_Identity=39.8230088495575, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=227, Percent_Identity=27.7533039647577, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI145693157, Length=269, Percent_Identity=24.907063197026, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788393, Length=237, Percent_Identity=27.0042194092827, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45585; Mature: 45585

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTLLLAAVGLIYSQRMALRRQRSLADEELFLQSERASVTLNAIHDGVLILRQDRSIEM
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEHHCCEEEEECCCCCCC
ANPIALRLVEMPAEEVLGQPCHEVFRLLGELASEFAEDPIAECFRTGAPVELQERMQLTT
CCHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHH
ARGKVHFVEGGIAPLLSRDGVLIGVVCALRDMGPLRQRTAEALEASETRVKEHLEKLSHV
HCCEEEEECCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLHTMGEMASGIAHELNQPLTAISNYCQAAMQMLEGVDDAPPQVHSALKLAVAQAGRAG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
EIIKRLRALVSKRAIETRLIDINQVVGNCMFLAEYDLRDRGIEVVQLLSGYSIPVMADSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCEEECHH
QLEQVVLNLLSNAMDALKDEPPMKRHVIIHTRREGRKGILEVRDTGRGITPDLLEVLFHP
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHH
FFSTKPHGMGLGLSICQTIVEQYGGLISAENIPASGACFRIELPLALQAERQVS
HHCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCHHCCCCCCCC
>Mature Secondary Structure
MLLTLLLAAVGLIYSQRMALRRQRSLADEELFLQSERASVTLNAIHDGVLILRQDRSIEM
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEHHCCEEEEECCCCCCC
ANPIALRLVEMPAEEVLGQPCHEVFRLLGELASEFAEDPIAECFRTGAPVELQERMQLTT
CCHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHH
ARGKVHFVEGGIAPLLSRDGVLIGVVCALRDMGPLRQRTAEALEASETRVKEHLEKLSHV
HCCEEEEECCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLHTMGEMASGIAHELNQPLTAISNYCQAAMQMLEGVDDAPPQVHSALKLAVAQAGRAG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
EIIKRLRALVSKRAIETRLIDINQVVGNCMFLAEYDLRDRGIEVVQLLSGYSIPVMADSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCEEECHH
QLEQVVLNLLSNAMDALKDEPPMKRHVIIHTRREGRKGILEVRDTGRGITPDLLEVLFHP
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHH
FFSTKPHGMGLGLSICQTIVEQYGGLISAENIPASGACFRIELPLALQAERQVS
HHCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]