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The map label for this gene is degA [H]

Identifier: 34498506

GI number: 34498506

Start: 3328088

End: 3329074

Strand: Reverse

Name: degA [H]

Synonym: CV_3051

Alternate gene names: 34498506

Gene position: 3329074-3328088 (Counterclockwise)

Preceding gene: 34498512

Following gene: 34498502

Centisome position: 70.07

GC content: 70.72

Gene sequence:

>987_bases
ATGACTACCAGCATCAAGGATGTCGCGTTGTTGGCCGGCGTATCGGTGGCCACCGTGTCGCGCGTGCTGGGCAACGGCCC
GGTCAGCGCCGCGCTGCGCGCCAAGGTGGAAGCCGCCGTCGCCCAGAGCGGCTACCGGCCCAACCTGTCGGCGCGGCGGC
TGCGCTCTCAGCACACGCATACCATAGGCCTGATCGTGTCAGACATCCGCAATCCGTTTTTCACTTCGGTGAGCCGGGCG
GTGGAGGACGTCGCCTACCGGTCGGGCATGCGCGTGATCCTGTGCAATACCGACGAGAACCCGGACAAGGAGGCGATGTA
CCTTCGGCTGATGCAGGAGGAGCGGGTGAGCGGGGTGATCTTCGCCGCCACGCGCGCCACCGCCGATTCGCTGGACGTCG
CCGGTCTGGGCTTCCCTGTGGTGATGATAGACCGCGCCGGACCTTCCGCCCGCTGCGACGCGGTGCTGCTGGACAACCGC
ACAGCGGCGGCCGAACTGGTCGATCACCTGATCGGGCGCGGCTACCGGCGCATCGGCGGCCTGTTCGGCAATACCAGCAG
CACCGGGGCCGAGCGGCACGCGGGCTTCCAGGAGGCGATGGAAAGGGCGGGCCTGGCGGCTGCGGCGCGCTTCGTCGCGC
CCAGCGCCGAGGCGGCGCAGGCGGCGGTGGCGGGCTGGTTGCGGGAGGCGGGCGCGCCTGAGGCGCTGATCGCCAGCAAC
GGCCTATTGCTGCTGGGCGCGGTGAAGGCGGTGCGGGCGGCCGGCATCGCCGTGCCGGGCGAACTGGCGCTGGCGGGCTT
CGACAACGATAGCTGGACCGAACTGGCGGATCCGGGCATGACGGTGATCGAGCAGCCGGTCGACGAGATCGGCCGCGCGG
CGATGGAAATGCTGCTGGAGCGGCTGCAGCAGCCGGAGCGGCCGCTGCGCAAGGTGGTGTTGGCCGGCCGGCTGGTGGCG
CGCGGCTCCAGCCGGACCAGGAACTGA

Upstream 100 bases:

>100_bases
CTTGTCGCGCAATTCGGAAAACTGTGGCGATTTTGCGCTTGCCAGCGCGGCGCGCGCTGGTGCAGCATCGCTTTCTTCAA
TTGAAAACGATTTCTCAGTC

Downstream 100 bases:

>100_bases
ATCAGCGCATCGGGCCGGTCAGCCGGGTCAGCGTGTCATCCTTGAGGAAACGGTGATGCCATACCGCGGCGGCGGCGTGC
AGTATGCTCAGCCACAGCAG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTTSIKDVALLAGVSVATVSRVLGNGPVSAALRAKVEAAVAQSGYRPNLSARRLRSQHTHTIGLIVSDIRNPFFTSVSRA
VEDVAYRSGMRVILCNTDENPDKEAMYLRLMQEERVSGVIFAATRATADSLDVAGLGFPVVMIDRAGPSARCDAVLLDNR
TAAAELVDHLIGRGYRRIGGLFGNTSSTGAERHAGFQEAMERAGLAAAARFVAPSAEAAQAAVAGWLREAGAPEALIASN
GLLLLGAVKAVRAAGIAVPGELALAGFDNDSWTELADPGMTVIEQPVDEIGRAAMEMLLERLQQPERPLRKVVLAGRLVA
RGSSRTRN

Sequences:

>Translated_328_residues
MTTSIKDVALLAGVSVATVSRVLGNGPVSAALRAKVEAAVAQSGYRPNLSARRLRSQHTHTIGLIVSDIRNPFFTSVSRA
VEDVAYRSGMRVILCNTDENPDKEAMYLRLMQEERVSGVIFAATRATADSLDVAGLGFPVVMIDRAGPSARCDAVLLDNR
TAAAELVDHLIGRGYRRIGGLFGNTSSTGAERHAGFQEAMERAGLAAAARFVAPSAEAAQAAVAGWLREAGAPEALIASN
GLLLLGAVKAVRAAGIAVPGELALAGFDNDSWTELADPGMTVIEQPVDEIGRAAMEMLLERLQQPERPLRKVVLAGRLVA
RGSSRTRN
>Mature_327_residues
TTSIKDVALLAGVSVATVSRVLGNGPVSAALRAKVEAAVAQSGYRPNLSARRLRSQHTHTIGLIVSDIRNPFFTSVSRAV
EDVAYRSGMRVILCNTDENPDKEAMYLRLMQEERVSGVIFAATRATADSLDVAGLGFPVVMIDRAGPSARCDAVLLDNRT
AAAELVDHLIGRGYRRIGGLFGNTSSTGAERHAGFQEAMERAGLAAAARFVAPSAEAAQAAVAGWLREAGAPEALIASNG
LLLLGAVKAVRAAGIAVPGELALAGFDNDSWTELADPGMTVIEQPVDEIGRAAMEMLLERLQQPERPLRKVVLAGRLVAR
GSSRTRN

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=328, Percent_Identity=32.6219512195122, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=31.9148936170213, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=35.1351351351351, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=30.4878048780488, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=31.8318318318318, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789068, Length=332, Percent_Identity=29.8192771084337, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1787906, Length=333, Percent_Identity=30.9309309309309, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI48994940, Length=304, Percent_Identity=28.9473684210526, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787580, Length=312, Percent_Identity=28.525641025641, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786540, Length=313, Percent_Identity=27.7955271565495, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786268, Length=317, Percent_Identity=26.1829652996845, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790715, Length=330, Percent_Identity=23.6363636363636, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790689, Length=318, Percent_Identity=26.1006289308176, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789456, Length=345, Percent_Identity=22.6086956521739, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34621; Mature: 34490

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2 ; PS00046 HISTONE_H2A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSIKDVALLAGVSVATVSRVLGNGPVSAALRAKVEAAVAQSGYRPNLSARRLRSQHTH
CCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCH
TIGLIVSDIRNPFFTSVSRAVEDVAYRSGMRVILCNTDENPDKEAMYLRLMQEERVSGVI
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCEEE
FAATRATADSLDVAGLGFPVVMIDRAGPSARCDAVLLDNRTAAAELVDHLIGRGYRRIGG
EEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHCC
LFGNTSSTGAERHAGFQEAMERAGLAAAARFVAPSAEAAQAAVAGWLREAGAPEALIASN
CCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEHHCC
GLLLLGAVKAVRAAGIAVPGELALAGFDNDSWTELADPGMTVIEQPVDEIGRAAMEMLLE
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLQQPERPLRKVVLAGRLVARGSSRTRN
HHHCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTSIKDVALLAGVSVATVSRVLGNGPVSAALRAKVEAAVAQSGYRPNLSARRLRSQHTH
CCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCH
TIGLIVSDIRNPFFTSVSRAVEDVAYRSGMRVILCNTDENPDKEAMYLRLMQEERVSGVI
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCEEE
FAATRATADSLDVAGLGFPVVMIDRAGPSARCDAVLLDNRTAAAELVDHLIGRGYRRIGG
EEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHCC
LFGNTSSTGAERHAGFQEAMERAGLAAAARFVAPSAEAAQAAVAGWLREAGAPEALIASN
CCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEHHCC
GLLLLGAVKAVRAAGIAVPGELALAGFDNDSWTELADPGMTVIEQPVDEIGRAAMEMLLE
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLQQPERPLRKVVLAGRLVARGSSRTRN
HHHCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]