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The map label for this gene is rfaD

Identifier: 34498493

GI number: 34498493

Start: 3316286

End: 3317287

Strand: Reverse

Name: rfaD

Synonym: CV_3038

Alternate gene names: 34498493

Gene position: 3317287-3316286 (Counterclockwise)

Preceding gene: 34498494

Following gene: 34498489

Centisome position: 69.82

GC content: 61.48

Gene sequence:

>1002_bases
ATGACTATCGTCGTCACCGGCGCCGCCGGCTTCATCGGCTCCAATCTGGTCAAGGGACTGAACCAGCGCGGCATCACCGA
CATCATCGCCGTCGACAATCTCAGCAACGGCGACAAGTTCCATAACCTGGTCGATTGCGAAATCTCGCACTACCTCGACA
AGCACGAATTCCTGCACCTGCTGCTGGATGGCGAGTACGAGGGCGAGCTGTCCGCCATCCTGCACCAGGGCGCGTGCTCG
GACACGATGAACCACGACGGCAAGTACATGATGGACAACAACTACCAGTACACGCTGGCGTTGTTCGACTATTGCCAGCA
CGAGGAAATCCAATTCCTGTACGCGTCCAGCGCCGCCACCTACGGCAAGGGCACGGTGTTCAAGGAAGAGCGCCAGCACG
AAGGCCCGCTCAACGTCTACGGCTACTCCAAGTTCCTGTTCGACCAAGTGCTGCGCCAGCGTATCAAGGAAGGCCTGTCC
GCCCAGGCTGTCGGTTTCCGCTACTTCAACGTCTACGGCCCGCGCGAGCAGCACAAGGGCCGCATGGCTTCGGTGGCCTT
TCACCACTTCAACCAGTACCGCGAACACGGCAAGGTCAAGCTGTTCGGCGGCTGGGATGGCTGGGAAAACGGCATGCAGA
GCCGTGATTTCGTGTCGGTGGAGGATGTGGTCAAGGTCAACCTGTTCTTCCTGGACAATCCCGGCAAGAGCGGCATCTAC
AATCTGGGCAGCGGCCGTTCGCAGCCGTTCAACGACGTGGCCGAGGCCACGGTGAATGCCTGCCGCCGCCACGAGGGCAA
GCCGGCGCTGACGCTGGCTGAAATGATCCAGCAGGGCATCGTCGAGTACATCGACTTCCCGGACGCGCTGAAGGGCAAGT
ACCAGAGCTTCACCCAGGCCGACATCGCCAAGCTGCGCGAAGCCGGCTACGCCGAGGCCATGCTGTCGGTTGCCGAGGGC
GTCGACCGCTATGTCGACTGGCTGATCGGCCGCCAGGGCTGA

Upstream 100 bases:

>100_bases
ACCAGCACGCCGGCCGAGCTGTCCTATCTGGCCCAACCCGGCGCGGGTTTCTGAATTTCCATCCGGGCCCGAGGGCCCGA
AGCAGAACAAGGATCTATAC

Downstream 100 bases:

>100_bases
TCGCCGGAACGATTCATCCGTCGGTGCAAAAACCGCGTCATCGTCGAAGATGGCGCGGTTTTTTTTGTTTCAGCGCCCGG
CGCTTTCCTGTTCCAGCCGC

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTIVVTGAAGFIGSNLVKGLNQRGITDIIAVDNLSNGDKFHNLVDCEISHYLDKHEFLHLLLDGEYEGELSAILHQGACS
DTMNHDGKYMMDNNYQYTLALFDYCQHEEIQFLYASSAATYGKGTVFKEERQHEGPLNVYGYSKFLFDQVLRQRIKEGLS
AQAVGFRYFNVYGPREQHKGRMASVAFHHFNQYREHGKVKLFGGWDGWENGMQSRDFVSVEDVVKVNLFFLDNPGKSGIY
NLGSGRSQPFNDVAEATVNACRRHEGKPALTLAEMIQQGIVEYIDFPDALKGKYQSFTQADIAKLREAGYAEAMLSVAEG
VDRYVDWLIGRQG

Sequences:

>Translated_333_residues
MTIVVTGAAGFIGSNLVKGLNQRGITDIIAVDNLSNGDKFHNLVDCEISHYLDKHEFLHLLLDGEYEGELSAILHQGACS
DTMNHDGKYMMDNNYQYTLALFDYCQHEEIQFLYASSAATYGKGTVFKEERQHEGPLNVYGYSKFLFDQVLRQRIKEGLS
AQAVGFRYFNVYGPREQHKGRMASVAFHHFNQYREHGKVKLFGGWDGWENGMQSRDFVSVEDVVKVNLFFLDNPGKSGIY
NLGSGRSQPFNDVAEATVNACRRHEGKPALTLAEMIQQGIVEYIDFPDALKGKYQSFTQADIAKLREAGYAEAMLSVAEG
VDRYVDWLIGRQG
>Mature_332_residues
TIVVTGAAGFIGSNLVKGLNQRGITDIIAVDNLSNGDKFHNLVDCEISHYLDKHEFLHLLLDGEYEGELSAILHQGACSD
TMNHDGKYMMDNNYQYTLALFDYCQHEEIQFLYASSAATYGKGTVFKEERQHEGPLNVYGYSKFLFDQVLRQRIKEGLSA
QAVGFRYFNVYGPREQHKGRMASVAFHHFNQYREHGKVKLFGGWDGWENGMQSRDFVSVEDVVKVNLFFLDNPGKSGIYN
LGSGRSQPFNDVAEATVNACRRHEGKPALTLAEMIQQGIVEYIDFPDALKGKYQSFTQADIAKLREAGYAEAMLSVAEGV
DRYVDWLIGRQG

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=327, Percent_Identity=55.9633027522936, Blast_Score=342, Evalue=2e-95,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_CHRVO (Q7NTL6)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902708.1
- ProteinModelPortal:   Q7NTL6
- SMR:   Q7NTL6
- GeneID:   2547524
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3038
- NMPDR:   fig|243365.1.peg.3038
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- PhylomeDB:   Q7NTL6
- ProtClustDB:   CLSK877767
- BioCyc:   CVIO243365:CV_3038-MONOMER
- BRENDA:   5.1.3.20
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 37518; Mature: 37386

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: ACT_SITE 140-140 ACT_SITE 179-179 BINDING 39-39 BINDING 54-54 BINDING 93-93 BINDING 144-144 BINDING 170-170 BINDING 171-171 BINDING 179-179 BINDING 181-181 BINDING 188-188 BINDING 215-215 BINDING 294-294

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVVTGAAGFIGSNLVKGLNQRGITDIIAVDNLSNGDKFHNLVDCEISHYLDKHEFLHL
CEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHEEE
LLDGEYEGELSAILHQGACSDTMNHDGKYMMDNNYQYTLALFDYCQHEEIQFLYASSAAT
EECCCCCCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEEHHHHCCCCEEEEEECCCCC
YGKGTVFKEERQHEGPLNVYGYSKFLFDQVLRQRIKEGLSAQAVGFRYFNVYGPREQHKG
CCCCCEEHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHCC
RMASVAFHHFNQYREHGKVKLFGGWDGWENGMQSRDFVSVEDVVKVNLFFLDNPGKSGIY
HHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCCCCHHHEEEEEEEEEECCCCCCCE
NLGSGRSQPFNDVAEATVNACRRHEGKPALTLAEMIQQGIVEYIDFPDALKGKYQSFTQA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DIAKLREAGYAEAMLSVAEGVDRYVDWLIGRQG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIVVTGAAGFIGSNLVKGLNQRGITDIIAVDNLSNGDKFHNLVDCEISHYLDKHEFLHL
EEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHEEE
LLDGEYEGELSAILHQGACSDTMNHDGKYMMDNNYQYTLALFDYCQHEEIQFLYASSAAT
EECCCCCCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEEHHHHCCCCEEEEEECCCCC
YGKGTVFKEERQHEGPLNVYGYSKFLFDQVLRQRIKEGLSAQAVGFRYFNVYGPREQHKG
CCCCCEEHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHCC
RMASVAFHHFNQYREHGKVKLFGGWDGWENGMQSRDFVSVEDVVKVNLFFLDNPGKSGIY
HHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCCCCHHHEEEEEEEEEECCCCCCCE
NLGSGRSQPFNDVAEATVNACRRHEGKPALTLAEMIQQGIVEYIDFPDALKGKYQSFTQA
ECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DIAKLREAGYAEAMLSVAEGVDRYVDWLIGRQG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782