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The map label for this gene is crp [H]

Identifier: 34498442

GI number: 34498442

Start: 3264094

End: 3264780

Strand: Reverse

Name: crp [H]

Synonym: CV_2987

Alternate gene names: 34498442

Gene position: 3264780-3264094 (Counterclockwise)

Preceding gene: 34498443

Following gene: 34498441

Centisome position: 68.72

GC content: 59.24

Gene sequence:

>687_bases
ATGAATCAAGAACAAATTAGGCAGATGTTGCGGCAGACGCCGCTGTTTGGTCATTTGACGGACGAGATGCTGGAAAGGGC
TCTGGAGTGTTCGGAAACGGTCCGTCTGGGCAAGGGCGAGTCTCTCTATGTCGAGGGGGATGAATCCGATTCCGTGGGCT
TGCTGGTGGCGGGGTCCATGCAGCTGTTCGTCGCCGACGAGGGCGGCAAGAAGTACATATTGCAGATGGCCATGCCTGGC
GGAATGGTTGGAATGGAGTCCTTCGTCGATGGCGGTTGCCGCAGCAGCAACGCGGTGGCAGACGAGGATTGCGTGTTGCT
GATGTTTTCCCGGGAGGTGCTGAAAGGCCTGTTCGACAGCGAGGACGGCCTGCCCGGTTGCGAGCGGGTGCGCGGCAACA
TCATGTGCTACCTGGTTTCCCTGGTGCGCCATATGACCACGCTGGCGCGCAACCTGGCCCTGCTGGACGTTTACGGCCGG
GTGCGCATCCTGATCAACCAGATGCTGATAGAGCAGGATGGCGTGCTGATGCTGCGCAAGCAGTTGACCCAGCAGGAGAT
TGCCGACCAGATCGGCTCATCGCGCGAGATGGTGGCGCGGATTCTGAAGGAGCTGGTCTACGGCCATTACATCCGCCTGG
AAAATCGCCGCATCGTGGTGTTGAAGATGCTGCCCGAGAATTTCTGA

Upstream 100 bases:

>100_bases
GCATATGAGCCACCTCTCATATCGCAAACAATAAATAATGACTTGGGCATGACTGTGCCTTCGAGGTTGCGGTGCCGGTA
GTCGGGGACGTGTTACCGAG

Downstream 100 bases:

>100_bases
GCCGAAGGTGGCGCCGCGCGGCATCGGGCCGCGCGGCTTTTTGCGCGCGTGGTTTAAGTTTGGCGGCCTGGGGTCGCCTT
GTATCGGTATCACACCCATA

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MNQEQIRQMLRQTPLFGHLTDEMLERALECSETVRLGKGESLYVEGDESDSVGLLVAGSMQLFVADEGGKKYILQMAMPG
GMVGMESFVDGGCRSSNAVADEDCVLLMFSREVLKGLFDSEDGLPGCERVRGNIMCYLVSLVRHMTTLARNLALLDVYGR
VRILINQMLIEQDGVLMLRKQLTQQEIADQIGSSREMVARILKELVYGHYIRLENRRIVVLKMLPENF

Sequences:

>Translated_228_residues
MNQEQIRQMLRQTPLFGHLTDEMLERALECSETVRLGKGESLYVEGDESDSVGLLVAGSMQLFVADEGGKKYILQMAMPG
GMVGMESFVDGGCRSSNAVADEDCVLLMFSREVLKGLFDSEDGLPGCERVRGNIMCYLVSLVRHMTTLARNLALLDVYGR
VRILINQMLIEQDGVLMLRKQLTQQEIADQIGSSREMVARILKELVYGHYIRLENRRIVVLKMLPENF
>Mature_228_residues
MNQEQIRQMLRQTPLFGHLTDEMLERALECSETVRLGKGESLYVEGDESDSVGLLVAGSMQLFVADEGGKKYILQMAMPG
GMVGMESFVDGGCRSSNAVADEDCVLLMFSREVLKGLFDSEDGLPGCERVRGNIMCYLVSLVRHMTTLARNLALLDVYGR
VRILINQMLIEQDGVLMLRKQLTQQEIADQIGSSREMVARILKELVYGHYIRLENRRIVVLKMLPENF

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=212, Percent_Identity=28.3018867924528, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25668; Mature: 25668

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQEQIRQMLRQTPLFGHLTDEMLERALECSETVRLGKGESLYVEGDESDSVGLLVAGSM
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCE
QLFVADEGGKKYILQMAMPGGMVGMESFVDGGCRSSNAVADEDCVLLMFSREVLKGLFDS
EEEEECCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCC
EDGLPGCERVRGNIMCYLVSLVRHMTTLARNLALLDVYGRVRILINQMLIEQDGVLMLRK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QLTQQEIADQIGSSREMVARILKELVYGHYIRLENRRIVVLKMLPENF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCEEEEEEECCCCC
>Mature Secondary Structure
MNQEQIRQMLRQTPLFGHLTDEMLERALECSETVRLGKGESLYVEGDESDSVGLLVAGSM
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCE
QLFVADEGGKKYILQMAMPGGMVGMESFVDGGCRSSNAVADEDCVLLMFSREVLKGLFDS
EEEEECCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCC
EDGLPGCERVRGNIMCYLVSLVRHMTTLARNLALLDVYGRVRILINQMLIEQDGVLMLRK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QLTQQEIADQIGSSREMVARILKELVYGHYIRLENRRIVVLKMLPENF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]