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The map label for this gene is fliY [H]

Identifier: 34498424

GI number: 34498424

Start: 3248177

End: 3248968

Strand: Reverse

Name: fliY [H]

Synonym: CV_2969

Alternate gene names: 34498424

Gene position: 3248968-3248177 (Counterclockwise)

Preceding gene: 34498426

Following gene: 34498421

Centisome position: 68.38

GC content: 66.29

Gene sequence:

>792_bases
ATGAGCGTGGCGGGTCGGAAAGCATGGGCGCTTTGCGTGGTGATGCTGTTGTCGCTGGCTCCTGCGCAGGGGGGCGGCGA
CGAGTGGGCGCGCATCCGGAAGGATGGCGCGCTGTTGATCGGCCTGGAGGGCACCTTTCCGCCCTATAGCCAGTTGGCGG
GCGGGCAGCTGCAGGGTTTCGAGGTGGATTTCGGCCGGGCGCTGGCTGCCCAGATGGGTGTAACGGCGCGTTTTCAGACC
ATGGTGTGGGACCATATGCTGGACAGCCTGGCGGCGGGCAGGCTGGATGCGGTGATCAACCAGGTGGTGGCGACGCCCGC
GCGCGGCAAACGCTTCGATTTTTCCATTCCCTATACCTATTCCGGCCTGCAGCTGGTGGTGCGCAAGCCGGATCTGACGC
GCTTTCGCGGCGCAAGGGATTTGGCCGGCGCCCATGTCGGCGTCAGCGCGGGCACCAATTACGAGCAATGGCTGCGAGAC
AACGCGCCTCAGGCCCACATTCACCTTTACCAGGATGATCCGACCAAGTTCGACGGCCTGCGCCGCGGCGAAGTGGACGC
CATCGTGATCAACCGGCTGGCGGCTTTCAACCTGGCGCTGAAGAGCCAGGGCGAACTGGTGCCGACCGGCCGCGTGCTGG
CGCGCGAGGACGCCTGCGTGGTGTTGCGCAAGGGCAATCCGCGGCTGCTGGCGGAGGTGAACCGGGCGATAGGCCACCTG
CAGCGCGACGGCACGCTGCAGGCGCTGTCGCGCAAATGGTTCAAGGCCGACATCGTCAGCCGGCCGCAATAG

Upstream 100 bases:

>100_bases
AGGGGTTTTCAGCGTTTTTTGCTGGCTGCCGGGCCGGCATCGCCTATTTTGTAAAGAATGATCTTGCCCTTAGTGCCAGT
GGGGCGGGCGGGGAGGCGAT

Downstream 100 bases:

>100_bases
CCGGCTTGCGGCAGCGAGGGCCGCTTTGTCAGCCGTACACGTCCAGCTGGCTGATCATGCCGGCCTGCCCGTCCTCCTTC
ACGAACACGCCGGCGCGCCG

Product: amino acid ABC transporter substrate-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSVAGRKAWALCVVMLLSLAPAQGGGDEWARIRKDGALLIGLEGTFPPYSQLAGGQLQGFEVDFGRALAAQMGVTARFQT
MVWDHMLDSLAAGRLDAVINQVVATPARGKRFDFSIPYTYSGLQLVVRKPDLTRFRGARDLAGAHVGVSAGTNYEQWLRD
NAPQAHIHLYQDDPTKFDGLRRGEVDAIVINRLAAFNLALKSQGELVPTGRVLAREDACVVLRKGNPRLLAEVNRAIGHL
QRDGTLQALSRKWFKADIVSRPQ

Sequences:

>Translated_263_residues
MSVAGRKAWALCVVMLLSLAPAQGGGDEWARIRKDGALLIGLEGTFPPYSQLAGGQLQGFEVDFGRALAAQMGVTARFQT
MVWDHMLDSLAAGRLDAVINQVVATPARGKRFDFSIPYTYSGLQLVVRKPDLTRFRGARDLAGAHVGVSAGTNYEQWLRD
NAPQAHIHLYQDDPTKFDGLRRGEVDAIVINRLAAFNLALKSQGELVPTGRVLAREDACVVLRKGNPRLLAEVNRAIGHL
QRDGTLQALSRKWFKADIVSRPQ
>Mature_262_residues
SVAGRKAWALCVVMLLSLAPAQGGGDEWARIRKDGALLIGLEGTFPPYSQLAGGQLQGFEVDFGRALAAQMGVTARFQTM
VWDHMLDSLAAGRLDAVINQVVATPARGKRFDFSIPYTYSGLQLVVRKPDLTRFRGARDLAGAHVGVSAGTNYEQWLRDN
APQAHIHLYQDDPTKFDGLRRGEVDAIVINRLAAFNLALKSQGELVPTGRVLAREDACVVLRKGNPRLLAEVNRAIGHLQ
RDGTLQALSRKWFKADIVSRPQ

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=49.5652173913044, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1787085, Length=221, Percent_Identity=29.8642533936652, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787088, Length=219, Percent_Identity=27.8538812785388, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788649, Length=239, Percent_Identity=26.3598326359833, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787031, Length=221, Percent_Identity=23.9819004524887, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=26.4957264957265, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28784; Mature: 28653

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS01039 SBP_BACTERIAL_3 ; PS00287 CYSTATIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAGRKAWALCVVMLLSLAPAQGGGDEWARIRKDGALLIGLEGTFPPYSQLAGGQLQGF
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCCCE
EVDFGRALAAQMGVTARFQTMVWDHMLDSLAAGRLDAVINQVVATPARGKRFDFSIPYTY
EECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
SGLQLVVRKPDLTRFRGARDLAGAHVGVSAGTNYEQWLRDNAPQAHIHLYQDDPTKFDGL
CCEEEEEECCCHHHHCCCHHHCCCEEECCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCC
RRGEVDAIVINRLAAFNLALKSQGELVPTGRVLAREDACVVLRKGNPRLLAEVNRAIGHL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHH
QRDGTLQALSRKWFKADIVSRPQ
CCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVAGRKAWALCVVMLLSLAPAQGGGDEWARIRKDGALLIGLEGTFPPYSQLAGGQLQGF
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCCCE
EVDFGRALAAQMGVTARFQTMVWDHMLDSLAAGRLDAVINQVVATPARGKRFDFSIPYTY
EECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
SGLQLVVRKPDLTRFRGARDLAGAHVGVSAGTNYEQWLRDNAPQAHIHLYQDDPTKFDGL
CCEEEEEECCCHHHHCCCHHHCCCEEECCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCC
RRGEVDAIVINRLAAFNLALKSQGELVPTGRVLAREDACVVLRKGNPRLLAEVNRAIGHL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHH
QRDGTLQALSRKWFKADIVSRPQ
CCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]