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The map label for this gene is lasA [H]

Identifier: 34498026

GI number: 34498026

Start: 2773127

End: 2774365

Strand: Direct

Name: lasA [H]

Synonym: CV_2571

Alternate gene names: 34498026

Gene position: 2773127-2774365 (Clockwise)

Preceding gene: 34498025

Following gene: 34498028

Centisome position: 58.37

GC content: 71.19

Gene sequence:

>1239_bases
ATGATGAAATCCGCCATTTCATATCTGTCCGCGCTGCTGATCGGCCTGGGCGCGGCGTCCGCCCACGCCGCCGAGGAGGC
CCAAGACCCCGCGCTGCGCCAGCAAGCCGCCGACGCCTTGGCGCGGCTCGCACCGCGACCGCTGGCCAAGGGAGATCTGC
CGCAGCCGCTGGTGGAAGTGAACCGCGAAAGCGCCGACCGCCGCTGGGTGATCGGATCGGCCACCCAGCCGCTGCCGCTC
ATGGCCGGCGCGGATGCCGACCAGGTGCCGTTGTCCCGCTTGTTCCTGGCGCGCCAGACCGACCAGGGCTGGCAGCTGGC
CATGGAAGGAGAAGACGGCTTCGCCGCGCTGCTGGAAGCCGCGCCGGACGGCTGGCTGAGCGCCGACGAGCGGCGCGCCT
GGCGCAGCCAGGCCATCCGCGCCAAGCGGCTGGCGCCCGACGCCACCGGCCTGGGCCTGCCCTGGCAGGAAGGCTCAAGC
TGGAGCATGACCGGCGGCCCGCACGGCTACAGCGGCGAGAGCCAGCCTTACGATTCCATAGACTTCGCCGGCGGCGACGG
CCGGGTGCTGGCACCGCAGGCCGGCGTGATCTACAAAAGCTGCCTGCGCAACGGCAGCGGACTGGTCAAGCTGGTGCACG
ACAACGGCTATTCCTCCACCTACTACCACATGATCAACCTCAACACGGTGGCCGACGGCCAGCGGATGGCCAAGGGCGCC
TATCTGGGCAAGATAGGCAACGGCCTGCCTTGCGGCGGAAGCACCACCGGCCCCCATGTGCACTTCTCGCTGATCCACCA
GGGCAAGGCCGAGCCGGTGAACCGCAAGTGGCTGGGCGGATGGCAATTCTTCTCCGGTGGCCGCGCCTACCAGGGCTACG
CCTCGCGCAACGGCAGCCGGGTGAACGTGGGCGGACGCCTGACCCACTACAGCGGCGGCAGCGACCCCGCGCCGACGCCG
AGCGGTTCCAGCGGCGTCGCCACGCCGCGCGGCGGCGACAGCCGCGTCAACCTGCGCGGCGGCCCCAGCCTGAGCGCGGA
AATCGTCGGCAGCGCGGCGCGCGGCGAACAGGTGGCGCTGCAATGCCACATCCGCGGCGAGGCGGTGGACGGCGTCTGGG
GCCGCACCGACATCTGGAACCGCACCGCCTCCGGCCACTGGATCAGCGACGGCTTCATCGACACCGGCAGCAACGCGCCG
GTGGTGCCGCCCTGCCCGTCATCGGCGCGGCCATGGTAA

Upstream 100 bases:

>100_bases
GTTCCCCTGCGCCCGCCTCTCTTAGGGACAGCTGAAAATAAAAATCACTCGCTATATGGTCGATCACGCTGTTCCTGTTC
AGAACGATAAGGAGTCCGTG

Downstream 100 bases:

>100_bases
ACGCCTAGCCGGCGCTTCGCCCTCCGCCGGGGACGGCCGCCCACGCGGCCGCGCCCCGGCGCATCATCAGCCAGTAAAGG
CCGGCCGCGCCCAGCCAGCC

Product: LasA protease

Products: NA

Alternate protein names: Staphylolytic protease [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MMKSAISYLSALLIGLGAASAHAAEEAQDPALRQQAADALARLAPRPLAKGDLPQPLVEVNRESADRRWVIGSATQPLPL
MAGADADQVPLSRLFLARQTDQGWQLAMEGEDGFAALLEAAPDGWLSADERRAWRSQAIRAKRLAPDATGLGLPWQEGSS
WSMTGGPHGYSGESQPYDSIDFAGGDGRVLAPQAGVIYKSCLRNGSGLVKLVHDNGYSSTYYHMINLNTVADGQRMAKGA
YLGKIGNGLPCGGSTTGPHVHFSLIHQGKAEPVNRKWLGGWQFFSGGRAYQGYASRNGSRVNVGGRLTHYSGGSDPAPTP
SGSSGVATPRGGDSRVNLRGGPSLSAEIVGSAARGEQVALQCHIRGEAVDGVWGRTDIWNRTASGHWISDGFIDTGSNAP
VVPPCPSSARPW

Sequences:

>Translated_412_residues
MMKSAISYLSALLIGLGAASAHAAEEAQDPALRQQAADALARLAPRPLAKGDLPQPLVEVNRESADRRWVIGSATQPLPL
MAGADADQVPLSRLFLARQTDQGWQLAMEGEDGFAALLEAAPDGWLSADERRAWRSQAIRAKRLAPDATGLGLPWQEGSS
WSMTGGPHGYSGESQPYDSIDFAGGDGRVLAPQAGVIYKSCLRNGSGLVKLVHDNGYSSTYYHMINLNTVADGQRMAKGA
YLGKIGNGLPCGGSTTGPHVHFSLIHQGKAEPVNRKWLGGWQFFSGGRAYQGYASRNGSRVNVGGRLTHYSGGSDPAPTP
SGSSGVATPRGGDSRVNLRGGPSLSAEIVGSAARGEQVALQCHIRGEAVDGVWGRTDIWNRTASGHWISDGFIDTGSNAP
VVPPCPSSARPW
>Mature_412_residues
MMKSAISYLSALLIGLGAASAHAAEEAQDPALRQQAADALARLAPRPLAKGDLPQPLVEVNRESADRRWVIGSATQPLPL
MAGADADQVPLSRLFLARQTDQGWQLAMEGEDGFAALLEAAPDGWLSADERRAWRSQAIRAKRLAPDATGLGLPWQEGSS
WSMTGGPHGYSGESQPYDSIDFAGGDGRVLAPQAGVIYKSCLRNGSGLVKLVHDNGYSSTYYHMINLNTVADGQRMAKGA
YLGKIGNGLPCGGSTTGPHVHFSLIHQGKAEPVNRKWLGGWQFFSGGRAYQGYASRNGSRVNVGGRLTHYSGGSDPAPTP
SGSSGVATPRGGDSRVNLRGGPSLSAEIVGSAARGEQVALQCHIRGEAVDGVWGRTDIWNRTASGHWISDGFIDTGSNAP
VVPPCPSSARPW

Specific function: Involved in proteolysis and elastolysis. Degradation of elastin is likely to contribute to the pathogenicity of P.aeruginosa [H]

COG id: COG0739

COG function: function code M; Membrane proteins related to metalloendopeptidases

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M23A family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR000841
- InterPro:   IPR016047
- InterPro:   IPR002886 [H]

Pfam domain/function: PF01551 Peptidase_M23 [H]

EC number: NA

Molecular weight: Translated: 43400; Mature: 43400

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKSAISYLSALLIGLGAASAHAAEEAQDPALRQQAADALARLAPRPLAKGDLPQPLVEV
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
NRESADRRWVIGSATQPLPLMAGADADQVPLSRLFLARQTDQGWQLAMEGEDGFAALLEA
CCCCCCCEEEECCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHH
APDGWLSADERRAWRSQAIRAKRLAPDATGLGLPWQEGSSWSMTGGPHGYSGESQPYDSI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
DFAGGDGRVLAPQAGVIYKSCLRNGSGLVKLVHDNGYSSTYYHMINLNTVADGQRMAKGA
EECCCCCEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHCH
YLGKIGNGLPCGGSTTGPHVHFSLIHQGKAEPVNRKWLGGWQFFSGGRAYQGYASRNGSR
HHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCEEECHHCCCCCE
VNVGGRLTHYSGGSDPAPTPSGSSGVATPRGGDSRVNLRGGPSLSAEIVGSAARGEQVAL
EECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCEEEE
QCHIRGEAVDGVWGRTDIWNRTASGHWISDGFIDTGSNAPVVPPCPSSARPW
EEEECCCEECCCCCCCHHCCCCCCCCEECCCCEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMKSAISYLSALLIGLGAASAHAAEEAQDPALRQQAADALARLAPRPLAKGDLPQPLVEV
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
NRESADRRWVIGSATQPLPLMAGADADQVPLSRLFLARQTDQGWQLAMEGEDGFAALLEA
CCCCCCCEEEECCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHH
APDGWLSADERRAWRSQAIRAKRLAPDATGLGLPWQEGSSWSMTGGPHGYSGESQPYDSI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
DFAGGDGRVLAPQAGVIYKSCLRNGSGLVKLVHDNGYSSTYYHMINLNTVADGQRMAKGA
EECCCCCEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHCH
YLGKIGNGLPCGGSTTGPHVHFSLIHQGKAEPVNRKWLGGWQFFSGGRAYQGYASRNGSR
HHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCEEECHHCCCCCE
VNVGGRLTHYSGGSDPAPTPSGSSGVATPRGGDSRVNLRGGPSLSAEIVGSAARGEQVAL
EECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCEEEE
QCHIRGEAVDGVWGRTDIWNRTASGHWISDGFIDTGSNAPVVPPCPSSARPW
EEEECCCEECCCCCCCHHCCCCCCCCEECCCCEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2836371; 2123032; 8932318; 10984043; 1597429 [H]