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The map label for this gene is narL [H]

Identifier: 34497990

GI number: 34497990

Start: 2727346

End: 2727993

Strand: Direct

Name: narL [H]

Synonym: CV_2535

Alternate gene names: 34497990

Gene position: 2727346-2727993 (Clockwise)

Preceding gene: 34497989

Following gene: 34497992

Centisome position: 57.4

GC content: 66.67

Gene sequence:

>648_bases
ATGACACAAGCCATACGCATTCTGTTGATTGACGATCACACGCTGTTCCGCAGCGGCCTGAAGGCGCTGCTGCAGCGGCA
GGACGATTTCCTGGTGGTCGGCGAAGCCGCCGACGGGCTGGACGGCGTCAAACAGGCCGAACTGCTGAAGCCCGACGTGG
TGCTGCTGGACCTGGACATGCCGGTGATGAACGGCCGCGAAGCGCTGGCGCAGATACTGAGCGGCTGTCCCAACGCCGCG
GTGCTGATGCTGACCGTGTCCGAGGACGGCGACGATCTGGCCGAATGCATGCGCGGCGGCGCCCGCGGCTATCTGCTGAA
GAACATCAACGCCGACTTCCTGCTGGACAGCATTCGCCGCGCGGTGGACGGCGACAGCGTGCTGTCGCCGGAAATGACCG
CCAAGTTGCTGGCCCGCCTGCGCCAACCGGCGGCCAAGGCCAGCGAAGCCGACGCGCTGACCCCGCGCGAACGGGAAACG
CTGGGCTGGCTGGCCCGCGGCGCCAGCAACAAGGAAATCGCCCGCGCGCTGGACCTGGCCGAGAGCACGGTCAAGGTCCA
CGTGCAGAACATCCTGCGCAAGCTGTCGCTGAGCAGCCGGGTGCAGGCGGCGGTTTATGCCGTGGAGCATGGGCTGGATA
AGGCCTGA

Upstream 100 bases:

>100_bases
AACGGGCCAAACGCATACACAGCCATATCCAGATCCGCTCGGCGCCCACGCAGGGCACCACGGTGGAGCTGACCCTGCCC
AAAGAGGAGCGCATCAGCGC

Downstream 100 bases:

>100_bases
GCCTTGCCCGGTTTGATACCCCCACAGCTATGCCATCATGGCGCGGCGCGGTCAAGGCCAACCCGCGCTCCCCTCCCATG
TGTTTATGAATTCAGCGTGG

Product: nitrate/nitrite response regulator protein narL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTQAIRILLIDDHTLFRSGLKALLQRQDDFLVVGEAADGLDGVKQAELLKPDVVLLDLDMPVMNGREALAQILSGCPNAA
VLMLTVSEDGDDLAECMRGGARGYLLKNINADFLLDSIRRAVDGDSVLSPEMTAKLLARLRQPAAKASEADALTPRERET
LGWLARGASNKEIARALDLAESTVKVHVQNILRKLSLSSRVQAAVYAVEHGLDKA

Sequences:

>Translated_215_residues
MTQAIRILLIDDHTLFRSGLKALLQRQDDFLVVGEAADGLDGVKQAELLKPDVVLLDLDMPVMNGREALAQILSGCPNAA
VLMLTVSEDGDDLAECMRGGARGYLLKNINADFLLDSIRRAVDGDSVLSPEMTAKLLARLRQPAAKASEADALTPRERET
LGWLARGASNKEIARALDLAESTVKVHVQNILRKLSLSSRVQAAVYAVEHGLDKA
>Mature_214_residues
TQAIRILLIDDHTLFRSGLKALLQRQDDFLVVGEAADGLDGVKQAELLKPDVVLLDLDMPVMNGREALAQILSGCPNAAV
LMLTVSEDGDDLAECMRGGARGYLLKNINADFLLDSIRRAVDGDSVLSPEMTAKLLARLRQPAAKASEADALTPRERETL
GWLARGASNKEIARALDLAESTVKVHVQNILRKLSLSSRVQAAVYAVEHGLDKA

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1787473, Length=201, Percent_Identity=45.273631840796, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=37.8640776699029, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=30.8056872037915, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=35, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786747, Length=201, Percent_Identity=25.3731343283582, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788712, Length=200, Percent_Identity=24, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787277, Length=84, Percent_Identity=35.7142857142857, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87082052, Length=161, Percent_Identity=29.8136645962733, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23251; Mature: 23120

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAIRILLIDDHTLFRSGLKALLQRQDDFLVVGEAADGLDGVKQAELLKPDVVLLDLDM
CCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCC
PVMNGREALAQILSGCPNAAVLMLTVSEDGDDLAECMRGGARGYLLKNINADFLLDSIRR
CCCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
AVDGDSVLSPEMTAKLLARLRQPAAKASEADALTPRERETLGWLARGASNKEIARALDLA
HHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
ESTVKVHVQNILRKLSLSSRVQAAVYAVEHGLDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQAIRILLIDDHTLFRSGLKALLQRQDDFLVVGEAADGLDGVKQAELLKPDVVLLDLDM
CCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCC
PVMNGREALAQILSGCPNAAVLMLTVSEDGDDLAECMRGGARGYLLKNINADFLLDSIRR
CCCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
AVDGDSVLSPEMTAKLLARLRQPAAKASEADALTPRERETLGWLARGASNKEIARALDLA
HHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
ESTVKVHVQNILRKLSLSSRVQAAVYAVEHGLDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]