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The map label for this gene is dapF

Identifier: 34497983

GI number: 34497983

Start: 2720444

End: 2721274

Strand: Direct

Name: dapF

Synonym: CV_2528

Alternate gene names: 34497983

Gene position: 2720444-2721274 (Clockwise)

Preceding gene: 34497982

Following gene: 34497984

Centisome position: 57.26

GC content: 68.23

Gene sequence:

>831_bases
ATGAAACTGAAATTCAGCAAGATGCACGGCCTGGGCAACGACTTCATGGTCATAGACGGCGTGCGCCAGACCGTTTCCCT
CACGCCGGAGCAGATACGCCTGCTCGGCAACCGCCAGCTCGGCATCGGCTTCGACCAGCTGCTGCTGGTGGAGCCGGCGC
GCGAGGCCGGCCACGACTTCCGTTACCGCATCTTCAACAACGACGGCGGCGAGGTCGAACAGTGCGGCAACGGCGCGCGC
TGCTTCGCCAAGTTCGTCCGCGACCAGGGCCTGACCGGCAAGAGCCGGATCGAAGTGGAAACCGCGCGCGGCGTGATCGC
GCCGGAATACCTGGGAGACGGCCTGGCCCGCGTCGACATGGGCGTGCCGCGCTTCCGGCCCGCCGACCTGCCCTTCCTGG
CCGACGCCGAAGCGCTGACCTACTCCCTGCCGTTGCCCGAAGGCCGCTGCGAAATCGGCATCGCGTCGATGGGCAATCCG
CACGCGGTGCAGCGGGTCGACGACGTCGATGCCGCGCCGGTGGCCGAGGTCGGGCCGGCCATCGAGAGCCACCCCGCCTT
TCCGCAACGGGTGAATGCCGGCTTCATGCAGATCGTCAACCGCGGCGAAATCCGATTGCGCGTCTACGAGCGTGGCGCCG
GCGAAACGCTGGCCTGCGGCACCGGCGCCTGCGCGGCGGTGGTCTGCGGCATACGCCAGGGCCTGCTGGACGCGCGCGTC
ACCGTGCATGCCCGCGGCGGCGACATCGTGATCGAATGGGCCGGCGAAGGCAGCCCGGTGATCATGACCGGCCCGGCGGT
CACCGTTTTCCAAGGCGAAATCGAACTTTAA

Upstream 100 bases:

>100_bases
CGCCCAATCTCCTCGTCCGCCCAGGCCCGCTCCCATAGATGTATCCCTCGATAGGCTGTGCTAAGCTGCCCCATCCACAA
TGCATCGCCACGCGACACCG

Downstream 100 bases:

>100_bases
GCCCCCCGGCGACACCAACCCATTTCCGCATCCCGATTAAGGAAAGGACAAGGCAGATGCACAGCGAACAAGTCCTGGCG
TTTCTCGACGAGCATCCCGA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKLKFSKMHGLGNDFMVIDGVRQTVSLTPEQIRLLGNRQLGIGFDQLLLVEPAREAGHDFRYRIFNNDGGEVEQCGNGAR
CFAKFVRDQGLTGKSRIEVETARGVIAPEYLGDGLARVDMGVPRFRPADLPFLADAEALTYSLPLPEGRCEIGIASMGNP
HAVQRVDDVDAAPVAEVGPAIESHPAFPQRVNAGFMQIVNRGEIRLRVYERGAGETLACGTGACAAVVCGIRQGLLDARV
TVHARGGDIVIEWAGEGSPVIMTGPAVTVFQGEIEL

Sequences:

>Translated_276_residues
MKLKFSKMHGLGNDFMVIDGVRQTVSLTPEQIRLLGNRQLGIGFDQLLLVEPAREAGHDFRYRIFNNDGGEVEQCGNGAR
CFAKFVRDQGLTGKSRIEVETARGVIAPEYLGDGLARVDMGVPRFRPADLPFLADAEALTYSLPLPEGRCEIGIASMGNP
HAVQRVDDVDAAPVAEVGPAIESHPAFPQRVNAGFMQIVNRGEIRLRVYERGAGETLACGTGACAAVVCGIRQGLLDARV
TVHARGGDIVIEWAGEGSPVIMTGPAVTVFQGEIEL
>Mature_276_residues
MKLKFSKMHGLGNDFMVIDGVRQTVSLTPEQIRLLGNRQLGIGFDQLLLVEPAREAGHDFRYRIFNNDGGEVEQCGNGAR
CFAKFVRDQGLTGKSRIEVETARGVIAPEYLGDGLARVDMGVPRFRPADLPFLADAEALTYSLPLPEGRCEIGIASMGNP
HAVQRVDDVDAAPVAEVGPAIESHPAFPQRVNAGFMQIVNRGEIRLRVYERGAGETLACGTGACAAVVCGIRQGLLDARV
TVHARGGDIVIEWAGEGSPVIMTGPAVTVFQGEIEL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=58.029197080292, Blast_Score=315, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_CHRVO (Q7NV18)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902198.1
- HSSP:   P44859
- ProteinModelPortal:   Q7NV18
- SMR:   Q7NV18
- GeneID:   2547608
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2528
- NMPDR:   fig|243365.1.peg.2528
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q7NV18
- BioCyc:   CVIO243365:CV_2528-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 29652; Mature: 29652

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKFSKMHGLGNDFMVIDGVRQTVSLTPEQIRLLGNRQLGIGFDQLLLVEPAREAGHDF
CCEECHHHCCCCCCEEEECCCHHHHHCCHHHHHEECCCEECCCCCEEEEECCCHHCCCCE
RYRIFNNDGGEVEQCGNGARCFAKFVRDQGLTGKSRIEVETARGVIAPEYLGDGLARVDM
EEEEEECCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCEECHHHHCCCCEEHHC
GVPRFRPADLPFLADAEALTYSLPLPEGRCEIGIASMGNPHAVQRVDDVDAAPVAEVGPA
CCCCCCCCCCCEEECCCCEEEECCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHCCC
IESHPAFPQRVNAGFMQIVNRGEIRLRVYERGAGETLACGTGACAAVVCGIRQGLLDARV
CCCCCCCHHHHCHHHHHHHCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCEE
TVHARGGDIVIEWAGEGSPVIMTGPAVTVFQGEIEL
EEEECCCEEEEEECCCCCCEEEECCEEEEEECEECC
>Mature Secondary Structure
MKLKFSKMHGLGNDFMVIDGVRQTVSLTPEQIRLLGNRQLGIGFDQLLLVEPAREAGHDF
CCEECHHHCCCCCCEEEECCCHHHHHCCHHHHHEECCCEECCCCCEEEEECCCHHCCCCE
RYRIFNNDGGEVEQCGNGARCFAKFVRDQGLTGKSRIEVETARGVIAPEYLGDGLARVDM
EEEEEECCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCEECHHHHCCCCEEHHC
GVPRFRPADLPFLADAEALTYSLPLPEGRCEIGIASMGNPHAVQRVDDVDAAPVAEVGPA
CCCCCCCCCCCEEECCCCEEEECCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHCCC
IESHPAFPQRVNAGFMQIVNRGEIRLRVYERGAGETLACGTGACAAVVCGIRQGLLDARV
CCCCCCCHHHHCHHHHHHHCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCEE
TVHARGGDIVIEWAGEGSPVIMTGPAVTVFQGEIEL
EEEECCCEEEEEECCCCCCEEEECCEEEEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782