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The map label for this gene is gcvA [H]

Identifier: 34497926

GI number: 34497926

Start: 2658252

End: 2659160

Strand: Direct

Name: gcvA [H]

Synonym: CV_2471

Alternate gene names: 34497926

Gene position: 2658252-2659160 (Clockwise)

Preceding gene: 34497923

Following gene: 34497932

Centisome position: 55.95

GC content: 66.67

Gene sequence:

>909_bases
ATGAGCGCTTACCCCCCTCTCAATTCTCTTCGCATTTTCGAGGCCGCGGCAAGGCTGGAAAGCTTTTCCGCCGCCGCCGG
CGAACTCTTCGTCACCCATGGCGCTGTGAGCAAACAGATTAAGCAGCTGGAAGACTGGCTGGGCGTGCAGCTGTTCGAGC
GCAGCGGCGGGCGCGTCAGGCTGACCGATGCCGGCTGGCGCTATCTGGTTCAGGTCCAGGACGGGCTGGATTTGATCGCC
AACGCCACGTCACAGCTGCTGCAACCCAATCGCCAGCGCAGGCTGACCATCAACTCGACGCCCACCTTCGCGGCATTCTG
GCTCTTGCCGCGGCTGGCAGGCTTCCGCTCCCAGCTGCCGGAGCTGGAGCTGCACCTGGTGACCTCCGACCGCGACCTTT
CGCGGCTGGACGCGCCATTCGACGTAGCGATCCGCCGCGGCCCCGGCGACTGGCCCGGCCATATCGCCAAGCCTTTCCTG
AAGGAGTGGGAGCTGCCGCTGTGCAGCCCGGCGCTGCTCAGCCGCCAGCCGCTGGCCCAGGCCGCAGATTTGACCCAGCA
CACCTTGCTGCATGCGGACACCCGCCCCACGGCCTGGCCGCGCTGGCTGACGCTGGCAGGCGCGCCCGAGCTCAAGCCCG
CCTGCAGCCTGCATTTCGACAAGTTCTCCCTGGCGCTGCAGGCCGCGGTGGATGGATTAGGCGTGGTGTTGGGCCCGCTG
CCCATGGCGCACGAACTGATAGCTGCCGGGAAACTGGTCTCCCCTTTGCCGGGTCCGGTGGTGACCGTGCGGGATTATTG
CTGGGTGGTGCCGCGCATGTCGGCCGAAGACGCGACAGTGGCGACTTTTTGCCGCTGGCTGGAGGGCGAGGCGGAAAAGG
CGGGCCTGTCAGGCGTTGAGAGTGGCTAA

Upstream 100 bases:

>100_bases
CGCTGCGGGATATGCACTTTGTAGTGACTGAAGATTACGCTTTTCGGCGCTTGCATACAAACGAGTAATCGCAACACGGA
TTGTGAGTTAAAATCACAGC

Downstream 100 bases:

>100_bases
CGAAATTCAGTTTGACGGCTGAGGCAAGGCGCGCCGACGCAGACCGCACCAGCGCGCAAAGGGCGCGGCTAGTACTGGCA
ACTGCGGCTCAACGCTTCAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSAYPPLNSLRIFEAAARLESFSAAAGELFVTHGAVSKQIKQLEDWLGVQLFERSGGRVRLTDAGWRYLVQVQDGLDLIA
NATSQLLQPNRQRRLTINSTPTFAAFWLLPRLAGFRSQLPELELHLVTSDRDLSRLDAPFDVAIRRGPGDWPGHIAKPFL
KEWELPLCSPALLSRQPLAQAADLTQHTLLHADTRPTAWPRWLTLAGAPELKPACSLHFDKFSLALQAAVDGLGVVLGPL
PMAHELIAAGKLVSPLPGPVVTVRDYCWVVPRMSAEDATVATFCRWLEGEAEKAGLSGVESG

Sequences:

>Translated_302_residues
MSAYPPLNSLRIFEAAARLESFSAAAGELFVTHGAVSKQIKQLEDWLGVQLFERSGGRVRLTDAGWRYLVQVQDGLDLIA
NATSQLLQPNRQRRLTINSTPTFAAFWLLPRLAGFRSQLPELELHLVTSDRDLSRLDAPFDVAIRRGPGDWPGHIAKPFL
KEWELPLCSPALLSRQPLAQAADLTQHTLLHADTRPTAWPRWLTLAGAPELKPACSLHFDKFSLALQAAVDGLGVVLGPL
PMAHELIAAGKLVSPLPGPVVTVRDYCWVVPRMSAEDATVATFCRWLEGEAEKAGLSGVESG
>Mature_301_residues
SAYPPLNSLRIFEAAARLESFSAAAGELFVTHGAVSKQIKQLEDWLGVQLFERSGGRVRLTDAGWRYLVQVQDGLDLIAN
ATSQLLQPNRQRRLTINSTPTFAAFWLLPRLAGFRSQLPELELHLVTSDRDLSRLDAPFDVAIRRGPGDWPGHIAKPFLK
EWELPLCSPALLSRQPLAQAADLTQHTLLHADTRPTAWPRWLTLAGAPELKPACSLHFDKFSLALQAAVDGLGVVLGPLP
MAHELIAAGKLVSPLPGPVVTVRDYCWVVPRMSAEDATVATFCRWLEGEAEKAGLSGVESG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=38.5416666666667, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=34.9315068493151, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=30.5084745762712, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.3793103448276, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786401, Length=264, Percent_Identity=30.3030303030303, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081978, Length=262, Percent_Identity=29.0076335877863, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=161, Percent_Identity=36.6459627329193, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789440, Length=267, Percent_Identity=26.5917602996255, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787128, Length=143, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33049; Mature: 32918

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAYPPLNSLRIFEAAARLESFSAAAGELFVTHGAVSKQIKQLEDWLGVQLFERSGGRVR
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHEECCCCEEE
LTDAGWRYLVQVQDGLDLIANATSQLLQPNRQRRLTINSTPTFAAFWLLPRLAGFRSQLP
EECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
ELELHLVTSDRDLSRLDAPFDVAIRRGPGDWPGHIAKPFLKEWELPLCSPALLSRQPLAQ
CEEEEEEECCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCHHH
AADLTQHTLLHADTRPTAWPRWLTLAGAPELKPACSLHFDKFSLALQAAVDGLGVVLGPL
HHHHHHHHHEECCCCCCCCCCEEEECCCCCCCCHHHEEHHHHHHHHHHHHCCHHHHHCCC
PMAHELIAAGKLVSPLPGPVVTVRDYCWVVPRMSAEDATVATFCRWLEGEAEKAGLSGVE
HHHHHHHHHCCCCCCCCCCEEEECHHEEECCCCCCCCHHHHHHHHHHCCCHHHCCCCCCC
SG
CC
>Mature Secondary Structure 
SAYPPLNSLRIFEAAARLESFSAAAGELFVTHGAVSKQIKQLEDWLGVQLFERSGGRVR
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHEECCCCEEE
LTDAGWRYLVQVQDGLDLIANATSQLLQPNRQRRLTINSTPTFAAFWLLPRLAGFRSQLP
EECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
ELELHLVTSDRDLSRLDAPFDVAIRRGPGDWPGHIAKPFLKEWELPLCSPALLSRQPLAQ
CEEEEEEECCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCHHH
AADLTQHTLLHADTRPTAWPRWLTLAGAPELKPACSLHFDKFSLALQAAVDGLGVVLGPL
HHHHHHHHHEECCCCCCCCCCEEEECCCCCCCCHHHEEHHHHHHHHHHHHCCHHHHHCCC
PMAHELIAAGKLVSPLPGPVVTVRDYCWVVPRMSAEDATVATFCRWLEGEAEKAGLSGVE
HHHHHHHHHCCCCCCCCCCEEEECHHEEECCCCCCCCHHHHHHHHHHCCCHHHCCCCCCC
SG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]