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The map label for this gene is rluF [H]

Identifier: 34497861

GI number: 34497861

Start: 2590708

End: 2591436

Strand: Direct

Name: rluF [H]

Synonym: CV_2406

Alternate gene names: 34497861

Gene position: 2590708-2591436 (Clockwise)

Preceding gene: 34497860

Following gene: 34497862

Centisome position: 54.53

GC content: 64.06

Gene sequence:

>729_bases
ATGGAACCTGTACGCCTGTCTAAACGCATGGCCGAGCTGGGGCTGTGCTCGCGCCGCGAGGCTGACAGCTATATCGAGCA
GGGCTGGGTCAAGGTGGATGGAGAAGTCGCCGTCCTGGGCCAGAAAGTGCTGCCGCACAATCAGATAGAGATCGACAAGA
AGGCGCAGGATGCCCAATCGGCCCGCGTGACCATCCTGCTCAACAAGCCGGTGGGCTACGTCTCCGGCCAGGCAGAGGAC
GGCTATCTGCCCGCCGCCGCGCTGATCAAGCCGGAAAATCGCTGGGCCGAGGATGGCAGCCGCAAGCGCTTCCAGCCATC
GCACTTGCGCGGCCTGGCGCCCGCCGGTCGCCTTGACATCGACTCCGTCGGCCTGCTGGTGCTGACGCAGGACGGGCGCA
TCGCCAAGCAGCTCATCGGCGAACATTCCGAAGTGGAGAAAGAATACCTGGTGCGCGTGGAGGGCGAGCTGACGCCCAGT
GGCCTGGCGCTGCTCAACCATGGCCTGGAGCTTGACGGCAAGAAGCTGCTGCCCGCCAAGGTGGGTTGGCAGAACGAAGA
CCAGCTGCGTTTCGTGCTGCGCGAAGGCAAGAAGCGGCAGATTCGCCGGATGTGCGAACTGGTCGGTTTGCGCGTGGTCG
GCCTGAAACGCATCTCGATCGGGAAAGTCAAGCTGGGCAACCTCCCGACGGGGCAGTGGCGCTATCTGCGCGACGACGAG
CAGTTCTGA

Upstream 100 bases:

>100_bases
TCAGGCGGGCGGCAATCTCATACAATAATGGTCGATTCAATAGCGGTCTCCACGCGCGAACGCGCGTCCGGAGCCCGCTT
TTTCATTACGGAAAGACCAA

Downstream 100 bases:

>100_bases
CCTGAGCGGCCGGAGGGGTGGGCATGTTCGAATTCTTGCTTCGCAAAATGGGATGGGACGCGGCCAGCCGTCTGCGAGTC
CGCGAGCAGGCGATGCAGAT

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MEPVRLSKRMAELGLCSRREADSYIEQGWVKVDGEVAVLGQKVLPHNQIEIDKKAQDAQSARVTILLNKPVGYVSGQAED
GYLPAAALIKPENRWAEDGSRKRFQPSHLRGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGEHSEVEKEYLVRVEGELTPS
GLALLNHGLELDGKKLLPAKVGWQNEDQLRFVLREGKKRQIRRMCELVGLRVVGLKRISIGKVKLGNLPTGQWRYLRDDE
QF

Sequences:

>Translated_242_residues
MEPVRLSKRMAELGLCSRREADSYIEQGWVKVDGEVAVLGQKVLPHNQIEIDKKAQDAQSARVTILLNKPVGYVSGQAED
GYLPAAALIKPENRWAEDGSRKRFQPSHLRGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGEHSEVEKEYLVRVEGELTPS
GLALLNHGLELDGKKLLPAKVGWQNEDQLRFVLREGKKRQIRRMCELVGLRVVGLKRISIGKVKLGNLPTGQWRYLRDDE
QF
>Mature_242_residues
MEPVRLSKRMAELGLCSRREADSYIEQGWVKVDGEVAVLGQKVLPHNQIEIDKKAQDAQSARVTILLNKPVGYVSGQAED
GYLPAAALIKPENRWAEDGSRKRFQPSHLRGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGEHSEVEKEYLVRVEGELTPS
GLALLNHGLELDGKKLLPAKVGWQNEDQLRFVLREGKKRQIRRMCELVGLRVVGLKRISIGKVKLGNLPTGQWRYLRDDE
QF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=37.344398340249, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1788510, Length=241, Percent_Identity=29.8755186721992, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787524, Length=243, Percent_Identity=33.7448559670782, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081838, Length=156, Percent_Identity=35.2564102564103, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27115; Mature: 27115

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPVRLSKRMAELGLCSRREADSYIEQGWVKVDGEVAVLGQKVLPHNQIEIDKKAQDAQS
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCEEEEEECCCCCCCCEEECCHHCCCCC
ARVTILLNKPVGYVSGQAEDGYLPAAALIKPENRWAEDGSRKRFQPSHLRGLAPAGRLDI
CEEEEEEECCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCC
DSVGLLVLTQDGRIAKQLIGEHSEVEKEYLVRVEGELTPSGLALLNHGLELDGKKLLPAK
CCCEEEEEECCCHHHHHHHCCHHHCCHHHEEEECCCCCCHHHHHHHCCCCCCCCEECCCC
VGWQNEDQLRFVLREGKKRQIRRMCELVGLRVVGLKRISIGKVKLGNLPTGQWRYLRDDE
CCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEECEEEECCCCCCCEEECCCCC
QF
CC
>Mature Secondary Structure
MEPVRLSKRMAELGLCSRREADSYIEQGWVKVDGEVAVLGQKVLPHNQIEIDKKAQDAQS
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCEEEEEECCCCCCCCEEECCHHCCCCC
ARVTILLNKPVGYVSGQAEDGYLPAAALIKPENRWAEDGSRKRFQPSHLRGLAPAGRLDI
CEEEEEEECCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCC
DSVGLLVLTQDGRIAKQLIGEHSEVEKEYLVRVEGELTPSGLALLNHGLELDGKKLLPAK
CCCEEEEEECCCHHHHHHHCCHHHCCHHHEEEECCCCCCHHHHHHHCCCCCCCCEECCCC
VGWQNEDQLRFVLREGKKRQIRRMCELVGLRVVGLKRISIGKVKLGNLPTGQWRYLRDDE
CCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEECEEEECCCCCCCEEECCCCC
QF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]