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The map label for this gene is nasA [H]

Identifier: 34497683

GI number: 34497683

Start: 2402723

End: 2403925

Strand: Direct

Name: nasA [H]

Synonym: CV_2228

Alternate gene names: 34497683

Gene position: 2402723-2403925 (Clockwise)

Preceding gene: 34497682

Following gene: 34497684

Centisome position: 50.57

GC content: 72.07

Gene sequence:

>1203_bases
ATGGACCGTACCTTCTGGCAGGCCGGGCACCGGCCGACGCTGGTTTCCGCCTTTCTGTACTTCGATCTGAGCTTCATGGC
CTGGTATCTGCTGGGACCGCTGCAGGTGCCCATCGCCGCCGCGCTGCAGCTTTCCACCCAGCAGCGCGGCCTGATGGTGG
CCACGCCCATCCTGGCCGGCGCGCTGCTGCGCCCGCTGATGGGCATGCTGGTGGACCGCATCGGCGCCAAGCGCGCCGGG
CTGCTCGGCCAGATCGTCGTCATCGCCGCGTTGCTCGGCGCCTGGCTCGCTAATCTGTCCAGCTTGGGCGGCATGCTGGC
CTTGGGCGCGCTGCTGGGCGTGGCCGGGGCCTCGTTCGCCGCCGCCTTGCCGCTGGCTTCGCGCTGGTATCCGCCGCGGC
ACCAGGGCACGGCGCTGGGCATCGCCGGGGCGGGCAATTCCGGCACCGTGCTGGCGGCGCTGTTCGCGCCGGCGCTGGCC
GCGGCCTTCGGCTGGCGCAATGTGTTCGGCCTGGCCTGCCTGCCCTTGCTGCTGGTGCTGGCGATCTACGCGGTCTGGGC
CAGGGAGGCGCCGGCCGCGGCGGGGCCCAAGCGCCTGTCCGACTACCTCTCCGTGCTGAAGGAGAAGGACGCCTGGTGGT
TCATGTTCTTCTACTCGATGACCTTCGGCGGTTTTGCCGGCTTCGCCGGCGCCTTGCCCGGCTATTTCCATGACCAGTTC
GGCTTCGACGCCAAAGCCGCCGGCCTCTACACCGCCGCCTGCGTGTTCGCCGGCTCGGTGATGCGGCCCTTGGGCGGCAT
GCTGGCCGACCGCATGGGTGGAACCCGCTCGCTGCTGGCGGTCTATCTGGCATGCGCGCTGTTGATGGCCGCCGCCGCGC
TGAACGCCGGCGGCGCTGGCGCGGCGCTGGCCCTGTTCGTGCTGGCCATGCTGTGCCTGGGCGCCGGCAACGGCGCGGTG
TTCCAGCTGGTGCCGCAGCGCTTCGGCCGGGAGCTGGGCGTGATGACCGGCCTGGTGGGCATGGCCGGCGGCATCGGCGG
CTTCCTGCTGGCGGCCGGGCTGGGCGCGGTCAAGCAGGCCAGCGGCGATTACGCGCCGGGCTTGTGGCTGTTCGCGGGCC
TCTGCCTGCTGGCCTGGCTCAGCCTGGCGGGCGTGCAGCGGCATTGGCGCGACAACTGGGCGGTGGCGGCCGCCCGCGTG
TGA

Upstream 100 bases:

>100_bases
CACTAGGGACAACGGCGTCCTCTCCTGTTTTGATGACGGGAGGGGGCGCCGTTTTGTTTTGACGATCTGCATGCAAACCA
TATGCATGCCGGGAGCGATC

Downstream 100 bases:

>100_bases
GGGGACAGGCATGGACATGGCCGCGCGGTGGCTTGCCCGCTACACGGCTGGAACATAGACCTGGCCAGCGGCGAGGCGCA
GGCGCCGGACAGCGGCTGCG

Product: nitrate/nitrite transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDRTFWQAGHRPTLVSAFLYFDLSFMAWYLLGPLQVPIAAALQLSTQQRGLMVATPILAGALLRPLMGMLVDRIGAKRAG
LLGQIVVIAALLGAWLANLSSLGGMLALGALLGVAGASFAAALPLASRWYPPRHQGTALGIAGAGNSGTVLAALFAPALA
AAFGWRNVFGLACLPLLLVLAIYAVWAREAPAAAGPKRLSDYLSVLKEKDAWWFMFFYSMTFGGFAGFAGALPGYFHDQF
GFDAKAAGLYTAACVFAGSVMRPLGGMLADRMGGTRSLLAVYLACALLMAAAALNAGGAGAALALFVLAMLCLGAGNGAV
FQLVPQRFGRELGVMTGLVGMAGGIGGFLLAAGLGAVKQASGDYAPGLWLFAGLCLLAWLSLAGVQRHWRDNWAVAAARV

Sequences:

>Translated_400_residues
MDRTFWQAGHRPTLVSAFLYFDLSFMAWYLLGPLQVPIAAALQLSTQQRGLMVATPILAGALLRPLMGMLVDRIGAKRAG
LLGQIVVIAALLGAWLANLSSLGGMLALGALLGVAGASFAAALPLASRWYPPRHQGTALGIAGAGNSGTVLAALFAPALA
AAFGWRNVFGLACLPLLLVLAIYAVWAREAPAAAGPKRLSDYLSVLKEKDAWWFMFFYSMTFGGFAGFAGALPGYFHDQF
GFDAKAAGLYTAACVFAGSVMRPLGGMLADRMGGTRSLLAVYLACALLMAAAALNAGGAGAALALFVLAMLCLGAGNGAV
FQLVPQRFGRELGVMTGLVGMAGGIGGFLLAAGLGAVKQASGDYAPGLWLFAGLCLLAWLSLAGVQRHWRDNWAVAAARV
>Mature_400_residues
MDRTFWQAGHRPTLVSAFLYFDLSFMAWYLLGPLQVPIAAALQLSTQQRGLMVATPILAGALLRPLMGMLVDRIGAKRAG
LLGQIVVIAALLGAWLANLSSLGGMLALGALLGVAGASFAAALPLASRWYPPRHQGTALGIAGAGNSGTVLAALFAPALA
AAFGWRNVFGLACLPLLLVLAIYAVWAREAPAAAGPKRLSDYLSVLKEKDAWWFMFFYSMTFGGFAGFAGALPGYFHDQF
GFDAKAAGLYTAACVFAGSVMRPLGGMLADRMGGTRSLLAVYLACALLMAAAALNAGGAGAALALFVLAMLCLGAGNGAV
FQLVPQRFGRELGVMTGLVGMAGGIGGFLLAAGLGAVKQASGDYAPGLWLFAGLCLLAWLSLAGVQRHWRDNWAVAAARV

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=410, Percent_Identity=27.8048780487805, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787743, Length=348, Percent_Identity=29.0229885057471, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41417; Mature: 41417

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure
MDRTFWQAGHRPTLVSAFLYFDLSFMAWYLLGPLQVPIAAALQLSTQQRGLMVATPILAG
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEHHHHHHH
ALLRPLMGMLVDRIGAKRAGLLGQIVVIAALLGAWLANLSSLGGMLALGALLGVAGASFA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALPLASRWYPPRHQGTALGIAGAGNSGTVLAALFAPALAAAFGWRNVFGLACLPLLLVL
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIYAVWAREAPAAAGPKRLSDYLSVLKEKDAWWFMFFYSMTFGGFAGFAGALPGYFHDQF
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
GFDAKAAGLYTAACVFAGSVMRPLGGMLADRMGGTRSLLAVYLACALLMAAAALNAGGAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
AALALFVLAMLCLGAGNGAVFQLVPQRFGRELGVMTGLVGMAGGIGGFLLAAGLGAVKQA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
SGDYAPGLWLFAGLCLLAWLSLAGVQRHWRDNWAVAAARV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]