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The map label for this gene is leuC2 [H]

Identifier: 34497624

GI number: 34497624

Start: 2341620

End: 2343050

Strand: Reverse

Name: leuC2 [H]

Synonym: CV_2169

Alternate gene names: 34497624

Gene position: 2343050-2341620 (Counterclockwise)

Preceding gene: 34497626

Following gene: 34497623

Centisome position: 49.32

GC content: 65.83

Gene sequence:

>1431_bases
GTGACCGCATTGCAAACCCCACCCCAAACGCTGTACCAGAAACTGGTCGAGACCCACACGGTGGCGCGTCTGGATTCGCA
GAACGTGCTGCTGTACGCCGATCTGCACATCATGAACGAGTACACCAGCCCGCAAGCCTTCGCCGGGCTGGCGGAAGCCG
GCCGCACAGTCTTGCAGCCCGGCCAGAACGTATCCGTGGTCGACCACATCATCCCGACCCACCCCGTGGCGATCCGCATC
ATCGAGGAGCCGAATTCGGCATTGCAGGCCTCGAACCTGAAGAAGAATTGCGAGCGCCACGGCATCCCGCTTTTCGACGC
CAACAACCGTTTTCAAGGCATCGAACATGTCATCGCGCCGGAACTGGGCATGATTCGTCCCGGCATGGTCGTCCTCTGCG
GCGACAGCCACACCACGACCTATGGCGCGCTGGGGGCCTTGGGCTTCGGCATCGGCACCTCCGAAGTCGAACACGTATTG
GCGACCCAGACCCTGGTGTATCGCCTGGCGCCAACCATGCGCATCCATGTAGCCGGCCGCTTGCCGCTCGGCACCACCGC
CAAGGACCTGATCCTGCTGATCATCAACCGGATCGGCGCGCACGGCGCGCGCGGCTACGCGGTTGAGTTCTGCGGCGAAG
CCATTGAAGCGTTGACGATAGAAGCGCGGATGACCTTGTGCAATATGGCGGTCGAGGCCGGCGCCCGCGGCGCGCTCATC
GCGCCGGATGCGCTGACGCTGGAATACGTCGCCCGTCATTGTCCGGACTTGGCCGGCGCGACGCTGGACGCCGCGAAGGC
GGACTGGCGCGCCCTGCGCAGCGACGACGGCGCCCCATTCGACCTGAAGCTTGAATTCCAGGCCGGCGACGTCGAGCCCT
ATGTGACATGGGGCACCAGCCCCGACCAATCCATTCCGATCAGCGGCCGCATCCCCGTCGCCGCGTCAGCGCCGGAGGGG
CCTGAGCGGCTCACCATGCAGCGGGCGCTGCGCTACACCGGCCTGGCCGAAGGCGCCCAATTGGAAGGCTTGCCCATCCA
GCGCGTCTTCATCGGCTCTTGCACCAATGCCCGCATCGAGGACTTGCGCGCCGTCGCGGACATCGTGCGCGGACGAAGCG
TGGCGCCTTCGGTGCGCGCCATGATCGTGCCGGGCTCAGGCGAGGTGCGCCGCCAGGCCGAACAGGAAGGCCTGGCTCAG
CTCTTCATCGAAGCCGGCTTTGAATGGCGTCAACCTGGCTGTTCCCTATGCCTGGCCATGAATGACGACGTGCTCAACCC
CGGCGAGCGATGCGCCTCCACCACCAACCGCAATTTCGAAGGCCGCCAAGGCCGGGATGCGATCACCCACCTGATGAGCC
CCGCCATGGCCGCCGCTGCCGCCGTCGCCGGCTGCATCACCGACGTTCGCAAACTGGGAGTCCGCGCATGA

Upstream 100 bases:

>100_bases
AAAAACAGTTTGATATTTCCATTATTGTACAAAAATCTGCAATCCAATAATTTGCCTCCCAAGGAAGCCTCCGCGCTTTC
AGGCTCATCGGGAGACAAGA

Downstream 100 bases:

>100_bases
CCATTCATACCCGAATCGAAGGCATGGCCGCGCCGCTGCCGGTCAGCAATCTGGACACCGACCAGATCATGCCGAAGCAA
TTCCTGCGCCGCATCGACAA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTALQTPPQTLYQKLVETHTVARLDSQNVLLYADLHIMNEYTSPQAFAGLAEAGRTVLQPGQNVSVVDHIIPTHPVAIRI
IEEPNSALQASNLKKNCERHGIPLFDANNRFQGIEHVIAPELGMIRPGMVVLCGDSHTTTYGALGALGFGIGTSEVEHVL
ATQTLVYRLAPTMRIHVAGRLPLGTTAKDLILLIINRIGAHGARGYAVEFCGEAIEALTIEARMTLCNMAVEAGARGALI
APDALTLEYVARHCPDLAGATLDAAKADWRALRSDDGAPFDLKLEFQAGDVEPYVTWGTSPDQSIPISGRIPVAASAPEG
PERLTMQRALRYTGLAEGAQLEGLPIQRVFIGSCTNARIEDLRAVADIVRGRSVAPSVRAMIVPGSGEVRRQAEQEGLAQ
LFIEAGFEWRQPGCSLCLAMNDDVLNPGERCASTTNRNFEGRQGRDAITHLMSPAMAAAAAVAGCITDVRKLGVRA

Sequences:

>Translated_476_residues
MTALQTPPQTLYQKLVETHTVARLDSQNVLLYADLHIMNEYTSPQAFAGLAEAGRTVLQPGQNVSVVDHIIPTHPVAIRI
IEEPNSALQASNLKKNCERHGIPLFDANNRFQGIEHVIAPELGMIRPGMVVLCGDSHTTTYGALGALGFGIGTSEVEHVL
ATQTLVYRLAPTMRIHVAGRLPLGTTAKDLILLIINRIGAHGARGYAVEFCGEAIEALTIEARMTLCNMAVEAGARGALI
APDALTLEYVARHCPDLAGATLDAAKADWRALRSDDGAPFDLKLEFQAGDVEPYVTWGTSPDQSIPISGRIPVAASAPEG
PERLTMQRALRYTGLAEGAQLEGLPIQRVFIGSCTNARIEDLRAVADIVRGRSVAPSVRAMIVPGSGEVRRQAEQEGLAQ
LFIEAGFEWRQPGCSLCLAMNDDVLNPGERCASTTNRNFEGRQGRDAITHLMSPAMAAAAAVAGCITDVRKLGVRA
>Mature_475_residues
TALQTPPQTLYQKLVETHTVARLDSQNVLLYADLHIMNEYTSPQAFAGLAEAGRTVLQPGQNVSVVDHIIPTHPVAIRII
EEPNSALQASNLKKNCERHGIPLFDANNRFQGIEHVIAPELGMIRPGMVVLCGDSHTTTYGALGALGFGIGTSEVEHVLA
TQTLVYRLAPTMRIHVAGRLPLGTTAKDLILLIINRIGAHGARGYAVEFCGEAIEALTIEARMTLCNMAVEAGARGALIA
PDALTLEYVARHCPDLAGATLDAAKADWRALRSDDGAPFDLKLEFQAGDVEPYVTWGTSPDQSIPISGRIPVAASAPEGP
ERLTMQRALRYTGLAEGAQLEGLPIQRVFIGSCTNARIEDLRAVADIVRGRSVAPSVRAMIVPGSGEVRRQAEQEGLAQL
FIEAGFEWRQPGCSLCLAMNDDVLNPGERCASTTNRNFEGRQGRDAITHLMSPAMAAAAAVAGCITDVRKLGVRA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=364, Percent_Identity=25.8241758241758, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI8659555, Length=381, Percent_Identity=26.7716535433071, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=54.5258620689655, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1787531, Length=357, Percent_Identity=28.2913165266106, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=364, Percent_Identity=28.021978021978, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=27.5482093663912, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI25149342, Length=302, Percent_Identity=27.4834437086093, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17568399, Length=386, Percent_Identity=26.1658031088083, Blast_Score=98, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=474, Percent_Identity=50.210970464135, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6320440, Length=482, Percent_Identity=26.3485477178423, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322261, Length=385, Percent_Identity=27.5324675324675, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=27.1468144044321, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281365315, Length=382, Percent_Identity=26.1780104712042, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI17864292, Length=382, Percent_Identity=26.1780104712042, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=26.1780104712042, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI28571643, Length=362, Percent_Identity=27.6243093922652, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645686, Length=503, Percent_Identity=25.6461232604374, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17137564, Length=393, Percent_Identity=25.9541984732824, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50985; Mature: 50854

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALQTPPQTLYQKLVETHTVARLDSQNVLLYADLHIMNEYTSPQAFAGLAEAGRTVLQP
CCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEEEEHHCCCCHHHHHHHHHCCHHHHCC
GQNVSVVDHIIPTHPVAIRIIEEPNSALQASNLKKNCERHGIPLFDANNRFQGIEHVIAP
CCCCCHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCC
ELGMIRPGMVVLCGDSHTTTYGALGALGFGIGTSEVEHVLATQTLVYRLAPTMRIHVAGR
CCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
LPLGTTAKDLILLIINRIGAHGARGYAVEFCGEAIEALTIEARMTLCNMAVEAGARGALI
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
APDALTLEYVARHCPDLAGATLDAAKADWRALRSDDGAPFDLKLEFQAGDVEPYVTWGTS
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCC
PDQSIPISGRIPVAASAPEGPERLTMQRALRYTGLAEGAQLEGLPIQRVFIGSCTNARIE
CCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCCCCHHHH
DLRAVADIVRGRSVAPSVRAMIVPGSGEVRRQAEQEGLAQLFIEAGFEWRQPGCSLCLAM
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
NDDVLNPGERCASTTNRNFEGRQGRDAITHLMSPAMAAAAAVAGCITDVRKLGVRA
CCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TALQTPPQTLYQKLVETHTVARLDSQNVLLYADLHIMNEYTSPQAFAGLAEAGRTVLQP
CCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEEEEHHCCCCHHHHHHHHHCCHHHHCC
GQNVSVVDHIIPTHPVAIRIIEEPNSALQASNLKKNCERHGIPLFDANNRFQGIEHVIAP
CCCCCHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCC
ELGMIRPGMVVLCGDSHTTTYGALGALGFGIGTSEVEHVLATQTLVYRLAPTMRIHVAGR
CCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
LPLGTTAKDLILLIINRIGAHGARGYAVEFCGEAIEALTIEARMTLCNMAVEAGARGALI
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
APDALTLEYVARHCPDLAGATLDAAKADWRALRSDDGAPFDLKLEFQAGDVEPYVTWGTS
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCC
PDQSIPISGRIPVAASAPEGPERLTMQRALRYTGLAEGAQLEGLPIQRVFIGSCTNARIE
CCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCCCCHHHH
DLRAVADIVRGRSVAPSVRAMIVPGSGEVRRQAEQEGLAQLFIEAGFEWRQPGCSLCLAM
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
NDDVLNPGERCASTTNRNFEGRQGRDAITHLMSPAMAAAAAVAGCITDVRKLGVRA
CCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA