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The map label for this gene is leuD1

Identifier: 34497623

GI number: 34497623

Start: 2340985

End: 2341623

Strand: Reverse

Name: leuD1

Synonym: CV_2168

Alternate gene names: 34497623

Gene position: 2341623-2340985 (Counterclockwise)

Preceding gene: 34497624

Following gene: 34497622

Centisome position: 49.29

GC content: 60.25

Gene sequence:

>639_bases
ATGACCATTCATACCCGAATCGAAGGCATGGCCGCGCCGCTGCCGGTCAGCAATCTGGACACCGACCAGATCATGCCGAA
GCAATTCCTGCGCCGCATCGACAAGGCGGGCTTGGCCGAAGGCCTGCTATACGACATGCGCTTCGGCCCGGATGGAAAGC
CCCGCCCGGAATTCGTTCTCAATCGGCCGGAATACGCCGCGGCCAGGATACTGGTCGCCGGTCCCAACTTCGGTTGCGGC
TCCAGCCGCGAGCATGCGGTCTGGGGCCTGATGCAATACGGCATCCAAGCGGTGATCGCGCCAAGCTTCGGCGAGATTTT
CTATTCGAACGCGATGAACAACAGCCTGATGCTGGTGGCGCTGGCCGAGGCCGATGTCGGGACCATTCTCGCCGACGTCT
CCGCGCCGGAAAACAGCTGGATCACGATCGATGTCGCATCCATGACGGTGCGAAGCAAAAGTCTGACGGCGTCTTTCTCG
CTGTCCGAACGCCATCGCCGGATGTTCCTCGAAGGCCTGGACATGATAGGCGCGACCTTGGCCATGCAAGACCAAATCCA
TGCTTTCGCCGCCAGACATTGGCAGCAGCGGCCCTGGCTGAAAGACATCGCATCCATGACAAAAAACCGTTTGGCGTAA

Upstream 100 bases:

>100_bases
AAGGCCGGGATGCGATCACCCACCTGATGAGCCCCGCCATGGCCGCCGCTGCCGCCGTCGCCGGCTGCATCACCGACGTT
CGCAAACTGGGAGTCCGCGC

Downstream 100 bases:

>100_bases
GCGGGCGCCCTGCCGGGCCACCAGCCCGGCGGCGCCGTCACGCCCTATCAGAGGAGACAAAAATGCATATAGAGGAAACC
GTTAGCAAGCCGCCGGCAGG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTIHTRIEGMAAPLPVSNLDTDQIMPKQFLRRIDKAGLAEGLLYDMRFGPDGKPRPEFVLNRPEYAAARILVAGPNFGCG
SSREHAVWGLMQYGIQAVIAPSFGEIFYSNAMNNSLMLVALAEADVGTILADVSAPENSWITIDVASMTVRSKSLTASFS
LSERHRRMFLEGLDMIGATLAMQDQIHAFAARHWQQRPWLKDIASMTKNRLA

Sequences:

>Translated_212_residues
MTIHTRIEGMAAPLPVSNLDTDQIMPKQFLRRIDKAGLAEGLLYDMRFGPDGKPRPEFVLNRPEYAAARILVAGPNFGCG
SSREHAVWGLMQYGIQAVIAPSFGEIFYSNAMNNSLMLVALAEADVGTILADVSAPENSWITIDVASMTVRSKSLTASFS
LSERHRRMFLEGLDMIGATLAMQDQIHAFAARHWQQRPWLKDIASMTKNRLA
>Mature_211_residues
TIHTRIEGMAAPLPVSNLDTDQIMPKQFLRRIDKAGLAEGLLYDMRFGPDGKPRPEFVLNRPEYAAARILVAGPNFGCGS
SREHAVWGLMQYGIQAVIAPSFGEIFYSNAMNNSLMLVALAEADVGTILADVSAPENSWITIDVASMTVRSKSLTASFSL
SERHRRMFLEGLDMIGATLAMQDQIHAFAARHWQQRPWLKDIASMTKNRLA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=187, Percent_Identity=43.3155080213904, Blast_Score=164, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6321429, Length=209, Percent_Identity=36.3636363636364, Blast_Score=138, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD1_CHRVO (Q7NW22)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901838.1
- ProteinModelPortal:   Q7NW22
- SMR:   Q7NW22
- GeneID:   2549463
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2168
- NMPDR:   fig|243365.1.peg.2168
- HOGENOM:   HBG304838
- OMA:   KGIDRSG
- PhylomeDB:   Q7NW22
- ProtClustDB:   CLSK922578
- BioCyc:   CVIO243365:CV_2168-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 23480; Mature: 23349

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHTRIEGMAAPLPVSNLDTDQIMPKQFLRRIDKAGLAEGLLYDMRFGPDGKPRPEFVL
CEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCEEE
NRPEYAAARILVAGPNFGCGSSREHAVWGLMQYGIQAVIAPSFGEIFYSNAMNNSLMLVA
CCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHCHHEEECCCHHHHHHHHCCCCCEEEEE
LAEADVGTILADVSAPENSWITIDVASMTVRSKSLTASFSLSERHRRMFLEGLDMIGATL
EECCCCCCEEEECCCCCCCEEEEEEHHEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHH
AMQDQIHAFAARHWQQRPWLKDIASMTKNRLA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIHTRIEGMAAPLPVSNLDTDQIMPKQFLRRIDKAGLAEGLLYDMRFGPDGKPRPEFVL
EEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCEEE
NRPEYAAARILVAGPNFGCGSSREHAVWGLMQYGIQAVIAPSFGEIFYSNAMNNSLMLVA
CCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHCHHEEECCCHHHHHHHHCCCCCEEEEE
LAEADVGTILADVSAPENSWITIDVASMTVRSKSLTASFSLSERHRRMFLEGLDMIGATL
EECCCCCCEEEECCCCCCCEEEEEEHHEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHH
AMQDQIHAFAARHWQQRPWLKDIASMTKNRLA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782