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The map label for this gene is ilvE [H]

Identifier: 34497549

GI number: 34497549

Start: 2280223

End: 2281146

Strand: Reverse

Name: ilvE [H]

Synonym: CV_2094

Alternate gene names: 34497549

Gene position: 2281146-2280223 (Counterclockwise)

Preceding gene: 34497550

Following gene: 34497548

Centisome position: 48.01

GC content: 63.42

Gene sequence:

>924_bases
ATGTCGATGGCTGATCGCGACGGATATATCTGGTATGACGGCAAGCTGGTGGACTGGCGCGACGCCACCACTCATGTGCT
GACCCACACCCTGCACTACGGCATGGGCGTGTTCGAAGGCGTGCGCGCCTACGAAACGCCGAAGGGTCCGGCCATCTTCC
GCCTGCAGGACCATACCGAGCGCCTGTTCCGCTCCGCCAAGATTCTGGGCATCGCCCTGCCCTTCACTCCGGAAGACATC
AACCAGGCCCACCTGGACGTGGTCAAGGCCAACGGCCTGAAATCCTGCTACTTCCGTCCGATGGCCTTCTACGGCTCCGG
CAAGCTGGGCGTGGCGCCGATGAAGAACGACGTGCGCGTGATCGTGGCTGCCTGGCCCTGGGGCGCCTACCTGGGCGAAG
AAGGCCTGGAGAAAGGCATCCGCGTGAAGACCAGCTCCTTCACCCGCCACCACGTCAACATCACCATGTGCAAGGCCAAG
GCCAACGGCAACTACATGAACTCCATCCTGGCCAACAACGAGGCCACCGCCGACGGCTACGACGAGGCGCTGCTGCTGGA
CGTGGACGGCTTCGTCGCCGAAGGCTCGGGCGAAAACATCTTCATCGTGCGCAAGGGCAAGCTTTATACTCCGGACCTGA
CCAGCGCGCTGGAAGGCATCACCCGCGACACCGTGGTGCAGATCGCCAAGGAGATGGGCCTGGAGCTGATCGAGAAGCGC
ATCACCCGCGACGAGGTCTACAGCGCCGACGAGGCCTTCTTCACCGGCACCGCCGCCGAGGTGACCCCGATCCGCGAGCT
GGACCGCCGCCCGATCGGCGCCGGCAGCCGCGGCCCGATCACCGCGGAAATCCAGAAGCGCTACTTCAATATCGTCAAGG
GTCTGGACGCCGAACACGAACACTGGCTCACATACGTGAAATAA

Upstream 100 bases:

>100_bases
CAAGTCCGCCGGCCGCGGTATACACTGGTCCATCAACACGGCGGCGGGCGCCTTGCCGCGCACCGCCGCCACACCAAGCA
CAACACAGGAGAAGACCGAG

Downstream 100 bases:

>100_bases
AACGGGTAATATCCGCACTGCGCGCCAGCAGCGACCGGTTCCGCCGGCCTGCTGGCAATGCATTTCGGACACCCGCTTGC
CGCAACAAGGAATGACCATG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSMADRDGYIWYDGKLVDWRDATTHVLTHTLHYGMGVFEGVRAYETPKGPAIFRLQDHTERLFRSAKILGIALPFTPEDI
NQAHLDVVKANGLKSCYFRPMAFYGSGKLGVAPMKNDVRVIVAAWPWGAYLGEEGLEKGIRVKTSSFTRHHVNITMCKAK
ANGNYMNSILANNEATADGYDEALLLDVDGFVAEGSGENIFIVRKGKLYTPDLTSALEGITRDTVVQIAKEMGLELIEKR
ITRDEVYSADEAFFTGTAAEVTPIRELDRRPIGAGSRGPITAEIQKRYFNIVKGLDAEHEHWLTYVK

Sequences:

>Translated_307_residues
MSMADRDGYIWYDGKLVDWRDATTHVLTHTLHYGMGVFEGVRAYETPKGPAIFRLQDHTERLFRSAKILGIALPFTPEDI
NQAHLDVVKANGLKSCYFRPMAFYGSGKLGVAPMKNDVRVIVAAWPWGAYLGEEGLEKGIRVKTSSFTRHHVNITMCKAK
ANGNYMNSILANNEATADGYDEALLLDVDGFVAEGSGENIFIVRKGKLYTPDLTSALEGITRDTVVQIAKEMGLELIEKR
ITRDEVYSADEAFFTGTAAEVTPIRELDRRPIGAGSRGPITAEIQKRYFNIVKGLDAEHEHWLTYVK
>Mature_306_residues
SMADRDGYIWYDGKLVDWRDATTHVLTHTLHYGMGVFEGVRAYETPKGPAIFRLQDHTERLFRSAKILGIALPFTPEDIN
QAHLDVVKANGLKSCYFRPMAFYGSGKLGVAPMKNDVRVIVAAWPWGAYLGEEGLEKGIRVKTSSFTRHHVNITMCKAKA
NGNYMNSILANNEATADGYDEALLLDVDGFVAEGSGENIFIVRKGKLYTPDLTSALEGITRDTVVQIAKEMGLELIEKRI
TRDEVYSADEAFFTGTAAEVTPIRELDRRPIGAGSRGPITAEIQKRYFNIVKGLDAEHEHWLTYVK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=289, Percent_Identity=25.6055363321799, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI48994963, Length=301, Percent_Identity=45.514950166113, Blast_Score=286, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17565728, Length=289, Percent_Identity=28.719723183391, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6322608, Length=308, Percent_Identity=27.9220779220779, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322002, Length=265, Percent_Identity=28.6792452830189, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34265; Mature: 34134

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00061 ADH_SHORT ; PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMADRDGYIWYDGKLVDWRDATTHVLTHTLHYGMGVFEGVRAYETPKGPAIFRLQDHTE
CCCCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
RLFRSAKILGIALPFTPEDINQAHLDVVKANGLKSCYFRPMAFYGSGKLGVAPMKNDVRV
HHHHCCEEEEEECCCCCCCCCHHHHEEEECCCCCHHHCCEEEEECCCCCCCCCCCCCEEE
IVAAWPWGAYLGEEGLEKGIRVKTSSFTRHHVNITMCKAKANGNYMNSILANNEATADGY
EEEECCCHHHHCHHHHHCCCEEEECCCEEEEEEEEEEEEECCCCHHHHHHCCCCCCCCCC
DEALLLDVDGFVAEGSGENIFIVRKGKLYTPDLTSALEGITRDTVVQIAKEMGLELIEKR
CCEEEEEECCEEECCCCCEEEEEECCCEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ITRDEVYSADEAFFTGTAAEVTPIRELDRRPIGAGSRGPITAEIQKRYFNIVKGLDAEHE
HHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
HWLTYVK
CEEEECC
>Mature Secondary Structure 
SMADRDGYIWYDGKLVDWRDATTHVLTHTLHYGMGVFEGVRAYETPKGPAIFRLQDHTE
CCCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
RLFRSAKILGIALPFTPEDINQAHLDVVKANGLKSCYFRPMAFYGSGKLGVAPMKNDVRV
HHHHCCEEEEEECCCCCCCCCHHHHEEEECCCCCHHHCCEEEEECCCCCCCCCCCCCEEE
IVAAWPWGAYLGEEGLEKGIRVKTSSFTRHHVNITMCKAKANGNYMNSILANNEATADGY
EEEECCCHHHHCHHHHHCCCEEEECCCEEEEEEEEEEEEECCCCHHHHHHCCCCCCCCCC
DEALLLDVDGFVAEGSGENIFIVRKGKLYTPDLTSALEGITRDTVVQIAKEMGLELIEKR
CCEEEEEECCEEECCCCCEEEEEECCCEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ITRDEVYSADEAFFTGTAAEVTPIRELDRRPIGAGSRGPITAEIQKRYFNIVKGLDAEHE
HHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
HWLTYVK
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]