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The map label for this gene is lepB [H]

Identifier: 34497519

GI number: 34497519

Start: 2252402

End: 2253373

Strand: Direct

Name: lepB [H]

Synonym: CV_2064

Alternate gene names: 34497519

Gene position: 2252402-2253373 (Clockwise)

Preceding gene: 34497518

Following gene: 34497520

Centisome position: 47.41

GC content: 61.32

Gene sequence:

>972_bases
GTGGGTGCAAACTTGTTCTGGGTGTTCTCGGCCGCGGTCGTGGTCGGCCTGTTGCTGATTCTGCTGGGCGGCAAGAAGCA
GGAAGGCGCCAAGGATTGGCCGCAATCGGTGCAGTGGGGCTATCTGGCCCTGGTGATCGGCGGCTTCGGCCTGCTGTCCG
ACTACATGAGCTTCACCGCGGTGATGCTGGTGTTCGTCGTGATCACCGGCGCGGTGTGGGCGCTGGACAAGTGGATGCTG
GCCAAGAAGCGCGCCGCCAGCGGCGCGCAGCCCGGCCATTTCGTCGATTATTCGCGCGGCTTCTTCCCGGTGATCCTCGT
GGTGTTCGTGCTGCGCTCCTTCCTGGTTGAACCGTTCCAGATTCCGTCCAGCTCGATGCGGCCGGGCCTGGTGGTCGGCG
ACTTCATCCTGGTCAACAAGTTCACCTACGGCATCCGCGTGCCGGTGCTGAACAACGTGCTGGTCCCGGTCAACCAGGTC
AAGCACGGCGACGTGGTGGTGTTCAATTATCCGCCCAATCCCAAGGTCAACTACATCAAGCGCGTGATCGGTTTGCCGGG
GGATACCGTCGAGTACCGCAACAAGCGGCTGACCGTGAACGGCAAGCCGCTGACCGATGTCGAGGACGGCACCTACGACT
ACATCGAGCAAGGCTTGGCGCAGATCACCGCGCAGCGTTTCCGGGAAAGTCAGGGCGGCAAGACCTACCATGTATTGAAC
AACGCCGAAGCGCCGGTGGTGGCCTTGAGCCAGGTGCAGGACTTTCCCTATCGCGACAATTGCCGCTACGATGACGATGG
CTTCGTCTGCAAGGTGCCGCAGGGCAATTACTTCATGATGGGCGACAACCGCGACAACAGCTCGGACGGCCGCTACTGGG
GCTTCGTCGACGACAAGCTGATGGTGGGCAAGGCCTTCATGATCTGGATGAATTTCACCCAGCTGTCCCGCATCGGCACG
CAGATCCAGTAA

Upstream 100 bases:

>100_bases
GGAAAAACAAAAGGCCGGCAAGAAGCGGATGAAACAGGTCGGCAACGTGGAAATTCCGCAAGAGGCCTTCCTTGCCATTC
TTCAAGTAGGGGACAAATAA

Downstream 100 bases:

>100_bases
AAACAATCAGGCCACGAGCGGCCATGACCGGGAGAAACGCATGAAGAAGCAGCAAGGCTTGTCGGTAATTGCGATTTTGT
TGGTTCTGGCGCTCGTCGGC

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MGANLFWVFSAAVVVGLLLILLGGKKQEGAKDWPQSVQWGYLALVIGGFGLLSDYMSFTAVMLVFVVITGAVWALDKWML
AKKRAASGAQPGHFVDYSRGFFPVILVVFVLRSFLVEPFQIPSSSMRPGLVVGDFILVNKFTYGIRVPVLNNVLVPVNQV
KHGDVVVFNYPPNPKVNYIKRVIGLPGDTVEYRNKRLTVNGKPLTDVEDGTYDYIEQGLAQITAQRFRESQGGKTYHVLN
NAEAPVVALSQVQDFPYRDNCRYDDDGFVCKVPQGNYFMMGDNRDNSSDGRYWGFVDDKLMVGKAFMIWMNFTQLSRIGT
QIQ

Sequences:

>Translated_323_residues
MGANLFWVFSAAVVVGLLLILLGGKKQEGAKDWPQSVQWGYLALVIGGFGLLSDYMSFTAVMLVFVVITGAVWALDKWML
AKKRAASGAQPGHFVDYSRGFFPVILVVFVLRSFLVEPFQIPSSSMRPGLVVGDFILVNKFTYGIRVPVLNNVLVPVNQV
KHGDVVVFNYPPNPKVNYIKRVIGLPGDTVEYRNKRLTVNGKPLTDVEDGTYDYIEQGLAQITAQRFRESQGGKTYHVLN
NAEAPVVALSQVQDFPYRDNCRYDDDGFVCKVPQGNYFMMGDNRDNSSDGRYWGFVDDKLMVGKAFMIWMNFTQLSRIGT
QIQ
>Mature_322_residues
GANLFWVFSAAVVVGLLLILLGGKKQEGAKDWPQSVQWGYLALVIGGFGLLSDYMSFTAVMLVFVVITGAVWALDKWMLA
KKRAASGAQPGHFVDYSRGFFPVILVVFVLRSFLVEPFQIPSSSMRPGLVVGDFILVNKFTYGIRVPVLNNVLVPVNQVK
HGDVVVFNYPPNPKVNYIKRVIGLPGDTVEYRNKRLTVNGKPLTDVEDGTYDYIEQGLAQITAQRFRESQGGKTYHVLNN
AEAPVVALSQVQDFPYRDNCRYDDDGFVCKVPQGNYFMMGDNRDNSSDGRYWGFVDDKLMVGKAFMIWMNFTQLSRIGTQ
IQ

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=321, Percent_Identity=34.2679127725857, Blast_Score=177, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 36152; Mature: 36021

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGANLFWVFSAAVVVGLLLILLGGKKQEGAKDWPQSVQWGYLALVIGGFGLLSDYMSFTA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLVFVVITGAVWALDKWMLAKKRAASGAQPGHFVDYSRGFFPVILVVFVLRSFLVEPFQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCC
IPSSSMRPGLVVGDFILVNKFTYGIRVPVLNNVLVPVNQVKHGDVVVFNYPPNPKVNYIK
CCCCCCCCCEEEEHHHEEEHEECEEECHHHCCEEECCCCCCCCCEEEEECCCCCHHHHHH
RVIGLPGDTVEYRNKRLTVNGKPLTDVEDGTYDYIEQGLAQITAQRFRESQGGKTYHVLN
HHHCCCCCCHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NAEAPVVALSQVQDFPYRDNCRYDDDGFVCKVPQGNYFMMGDNRDNSSDGRYWGFVDDKL
CCCCCEEEHHHHHCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCEEEEECCHH
MVGKAFMIWMNFTQLSRIGTQIQ
HHHHEEEEEHHHHHHHHHCCCCC
>Mature Secondary Structure 
GANLFWVFSAAVVVGLLLILLGGKKQEGAKDWPQSVQWGYLALVIGGFGLLSDYMSFTA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLVFVVITGAVWALDKWMLAKKRAASGAQPGHFVDYSRGFFPVILVVFVLRSFLVEPFQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCC
IPSSSMRPGLVVGDFILVNKFTYGIRVPVLNNVLVPVNQVKHGDVVVFNYPPNPKVNYIK
CCCCCCCCCEEEEHHHEEEHEECEEECHHHCCEEECCCCCCCCCEEEEECCCCCHHHHHH
RVIGLPGDTVEYRNKRLTVNGKPLTDVEDGTYDYIEQGLAQITAQRFRESQGGKTYHVLN
HHHCCCCCCHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NAEAPVVALSQVQDFPYRDNCRYDDDGFVCKVPQGNYFMMGDNRDNSSDGRYWGFVDDKL
CCCCCEEEHHHHHCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCEEEEECCHH
MVGKAFMIWMNFTQLSRIGTQIQ
HHHHEEEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]