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The map label for this gene is mii [H]

Identifier: 34497508

GI number: 34497508

Start: 2239686

End: 2240876

Strand: Reverse

Name: mii [H]

Synonym: CV_2053

Alternate gene names: 34497508

Gene position: 2240876-2239686 (Counterclockwise)

Preceding gene: 34497509

Following gene: 34497507

Centisome position: 47.17

GC content: 69.19

Gene sequence:

>1191_bases
ATGGCTCACCCCGCCCAGATCCGCATTCCCGCCGCTTATATGCGCGGCGGCACCAGCAAAGGCGTGTTCTTCCGGCTGCA
GGATCTGCCGGAAGCCGCCCGCCAGCCCGGCGCGGCGCGCGACGCGCTGCTGCTGCGCGTGATCGGCAGCCCGGACCCTT
ACGGCAAGCAGATAGACGGCATGGGCGGCGCGACGTCCAGCACCAGCAAGACCGTCATCGTGTCCCGATCCGCGCGCCCG
GACCACGACGTCGATTACCTGTTCGGCCAGGTCGCCATCGACAAGGCCTTCGTCGACTGGAGCGGCAATTGCGGCAACCT
GTCCGCCGCCGTCGGCCCTTTCGCGATCGCCAACGGCCTGATCGACCCCGCTCGCATCCCGGCAGACGGCTTCTGCACCG
TGCGCATCTGGCAGGCCAATATCGGCAAGACCATCGTCGCCCACGTGCCCGTCGCCAACGGCCAGGTGCAGGAAACCGGC
GACTTCGAGCTGGACGGCGTCACCTTCCCCGCCGCCGAAGTGCGGCTGGAATTTCTCGACCCGGCCGACGAGGGCGAAGG
CGACGGCGGCGCGATGTTCCCCACCGGCAAGCTGGTGGACACGCTGGAAGTGCCCGGCGTCGGCGCCTTTCCGGCCACGC
TGATCAATGCCGGCATCCCCACCATCTTCGTCAACGCCGCCGACATCGGCTATACCGGCACTGAGCTGCAGGACGCGATC
AACTCCGATCCGGCCGCGCTGGCGCGCTTCGAAACCATACGCGCCCACGGCGCGCTGAAGATGGGGCTGATCAAGCGCCT
GGACGAGATCGCCAGCCGCCAGCACACGCCCAAGATCGCCTTCGTCGCGCCGCCGGCCGACTACGTCTCGTCCAGCGGCA
AGCCGGTGAAAGCATCCGACATCGACGTGCTGGCGCGGGCGATGTCCATGGGCAAATTGCACCACGCGATGATGGGCACC
GCCGCGGTGGCCATAGGCACGGCGGCGGCCATTCCCGGCACGCTGGTCAACCTGGCCGCCGGCGGCGGAAAACGCCAGGC
GGTGCGCTTCGGCCACCCATCCGGCACGCTGCGCGTGGGCGCGCAAGCCCGCGAGGCACACGGCGAATGGGTGGTGACCA
AGGCGGAAATGAGCCGCAGCGCCCGGGTGCTGATGGAGGGCTGGGTGCGGGTGCCCGGGGACGCTTTCTAG

Upstream 100 bases:

>100_bases
TGCAGCGTTTCGCGCAGGACTTCCTGGCAGCGGCGCAAGCCTGATCAAGCAACACGGCCGGCGCCAGCCGGCCATTCAAC
AAAGAAACAGGATGGACAGC

Downstream 100 bases:

>100_bases
ACGCCGCTCCCTGCCGGCAAGGCAAACGATGGGGGTGTCGGGCATCCTCCCTGTTTTCACATCCCTCCGGCCAGGCCGCC
GGCGATTTTCGACGGGGTTT

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAHPAQIRIPAAYMRGGTSKGVFFRLQDLPEAARQPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSRSARP
DHDVDYLFGQVAIDKAFVDWSGNCGNLSAAVGPFAIANGLIDPARIPADGFCTVRIWQANIGKTIVAHVPVANGQVQETG
DFELDGVTFPAAEVRLEFLDPADEGEGDGGAMFPTGKLVDTLEVPGVGAFPATLINAGIPTIFVNAADIGYTGTELQDAI
NSDPAALARFETIRAHGALKMGLIKRLDEIASRQHTPKIAFVAPPADYVSSSGKPVKASDIDVLARAMSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGGKRQAVRFGHPSGTLRVGAQAREAHGEWVVTKAEMSRSARVLMEGWVRVPGDAF

Sequences:

>Translated_396_residues
MAHPAQIRIPAAYMRGGTSKGVFFRLQDLPEAARQPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSRSARP
DHDVDYLFGQVAIDKAFVDWSGNCGNLSAAVGPFAIANGLIDPARIPADGFCTVRIWQANIGKTIVAHVPVANGQVQETG
DFELDGVTFPAAEVRLEFLDPADEGEGDGGAMFPTGKLVDTLEVPGVGAFPATLINAGIPTIFVNAADIGYTGTELQDAI
NSDPAALARFETIRAHGALKMGLIKRLDEIASRQHTPKIAFVAPPADYVSSSGKPVKASDIDVLARAMSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGGKRQAVRFGHPSGTLRVGAQAREAHGEWVVTKAEMSRSARVLMEGWVRVPGDAF
>Mature_395_residues
AHPAQIRIPAAYMRGGTSKGVFFRLQDLPEAARQPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSRSARPD
HDVDYLFGQVAIDKAFVDWSGNCGNLSAAVGPFAIANGLIDPARIPADGFCTVRIWQANIGKTIVAHVPVANGQVQETGD
FELDGVTFPAAEVRLEFLDPADEGEGDGGAMFPTGKLVDTLEVPGVGAFPATLINAGIPTIFVNAADIGYTGTELQDAIN
SDPAALARFETIRAHGALKMGLIKRLDEIASRQHTPKIAFVAPPADYVSSSGKPVKASDIDVLARAMSMGKLHHAMMGTA
AVAIGTAAAIPGTLVNLAAGGGKRQAVRFGHPSGTLRVGAQAREAHGEWVVTKAEMSRSARVLMEGWVRVPGDAF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=385, Percent_Identity=34.8051948051948, Blast_Score=178, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41338; Mature: 41206

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHPAQIRIPAAYMRGGTSKGVFFRLQDLPEAARQPGAARDALLLRVIGSPDPYGKQIDG
CCCCCEEEECHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCC
MGGATSSTSKTVIVSRSARPDHDVDYLFGQVAIDKAFVDWSGNCGNLSAAVGPFAIANGL
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHEEEEEEEECCCCCCCCCHHHCHHHHHHCC
IDPARIPADGFCTVRIWQANIGKTIVAHVPVANGQVQETGDFELDGVTFPAAEVRLEFLD
CCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHHEEEEEEC
PADEGEGDGGAMFPTGKLVDTLEVPGVGAFPATLINAGIPTIFVNAADIGYTGTELQDAI
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHH
NSDPAALARFETIRAHGALKMGLIKRLDEIASRQHTPKIAFVAPPADYVSSSGKPVKASD
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCCCCCH
IDVLARAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGKRQAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEECCCCCCEEEC
AQAREAHGEWVVTKAEMSRSARVLMEGWVRVPGDAF
CHHHHCCCCEEEEHHHHCCHHHHHHHCCEECCCCCC
>Mature Secondary Structure 
AHPAQIRIPAAYMRGGTSKGVFFRLQDLPEAARQPGAARDALLLRVIGSPDPYGKQIDG
CCCCEEEECHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCC
MGGATSSTSKTVIVSRSARPDHDVDYLFGQVAIDKAFVDWSGNCGNLSAAVGPFAIANGL
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHEEEEEEEECCCCCCCCCHHHCHHHHHHCC
IDPARIPADGFCTVRIWQANIGKTIVAHVPVANGQVQETGDFELDGVTFPAAEVRLEFLD
CCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHHEEEEEEC
PADEGEGDGGAMFPTGKLVDTLEVPGVGAFPATLINAGIPTIFVNAADIGYTGTELQDAI
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHH
NSDPAALARFETIRAHGALKMGLIKRLDEIASRQHTPKIAFVAPPADYVSSSGKPVKASD
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCCCCCH
IDVLARAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGKRQAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEECCCCCCEEEC
AQAREAHGEWVVTKAEMSRSARVLMEGWVRVPGDAF
CHHHHCCCCEEEEHHHHCCHHHHHHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA