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The map label for this gene is ald [H]

Identifier: 34497477

GI number: 34497477

Start: 2203165

End: 2204289

Strand: Direct

Name: ald [H]

Synonym: CV_2022

Alternate gene names: 34497477

Gene position: 2203165-2204289 (Clockwise)

Preceding gene: 34497471

Following gene: 34497478

Centisome position: 46.37

GC content: 66.76

Gene sequence:

>1125_bases
ATGTTGATCGGCGTTCCGAAGGAAATCAAAAACCACGAGTACCGCGTGGGTCTGACCCCGTCCGGTGTCCGCGAGCTGGT
GGCGAACGGGCACAAGGTGCTGGTGCAGACCCACGCGGGTCTCGCGATAGGCTTTACCGACGAGCAATACATCCAGGCCG
GAGCCTCCATCGCCTCCAACGCCGAAGAGGTGTTCGAGCGCGCCGACATGATCGTCAAGGTCAAGGAGCCGCAGCCGGTG
GAGTGCAGGATGCTGCGTCCGGGCCAGATTCTTTTCACTTACCTGCATCTGGCGCCGGACCCGGAACAGACCAAGCTGCT
GATCGAGTCCGACTCGGTGGCCATCGCCTATGAAACGGTCACCGACGAGCGCGGCGGCCTGCCGCTGTTGGCGCCGATGT
CCGAAGTGGCCGGCCGCATGGCGATCCAGGCCGGCGCCCACGCGCTGGAAAAGGCCCAGGGCGGCCGCGGCGTGTTGCTG
GGCGGCGTGCCCGGCGTGGCCCCGGCCAAAGTGGTGGTGATAGGCGGCGGCGTGGTGGGGCTCAACGCCGCGCGGATGGC
GATGGGGGCGGGCGCAGACGTCACCATCCTGGACAAGTCCCTGAGCCGGCTCAAAGAGATAGACATGGTGTTCGGCGGCC
GCATCAAGACGCTGATGTCCAACGGCGCCAATATAGACGACAGCATCCGCGACGCCGACCTGGTGATCGGCGCGGTGCTG
GTGCCCGGCGCGGCGGCGCCCAAGCTGGTGACGCGCGCGATGCTGAAGACGATGAAGCCGGGCGCGGTGCTGGTGGATGT
GGCGATAGACCAGGGCGGCTGTTTCGAAACCAGCCGGCCGACCACCCACCAGGACCCGATCTACACCGTGGACGGCATCG
TCCACTACTGTGTGGCCAATATGCCCGGCGGCGTCGCCCGCACCTCCACCCAGGCGCTGACCAACGCCACGCTGCCGTAT
ACGCTGGAGCTGGCCAACAAGGGCTGGCGCCAGGCGCTGCTGGACAACGCCCACCTGCGCAACGGCCTCAACGTCTGCCG
CGGGCGGGTTACATACCAGGCGGTGGCCCGCGATTTGGGCCACGCCTACGTCGATCCGATAGAGGCCATCAAGGCCGCAA
ACTGA

Upstream 100 bases:

>100_bases
TTATAGTCAGAATCAAGTTGAAAGGGCAAGCGCGATGTGAATTTTCTTAAACGGCGTTTGTCTCCCTGATCAGGGCAAAA
AAACCAGTTTGGAGGCGACT

Downstream 100 bases:

>100_bases
CAAGGAGCGCTGCAAGCCATGTCGCAAGCAATGATAGGCATCCCGTGCGATCTGAAAATGATCGGTCTGTTGCCGTTCCA
CGCGGTGGGCGACAAATACA

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MLIGVPKEIKNHEYRVGLTPSGVRELVANGHKVLVQTHAGLAIGFTDEQYIQAGASIASNAEEVFERADMIVKVKEPQPV
ECRMLRPGQILFTYLHLAPDPEQTKLLIESDSVAIAYETVTDERGGLPLLAPMSEVAGRMAIQAGAHALEKAQGGRGVLL
GGVPGVAPAKVVVIGGGVVGLNAARMAMGAGADVTILDKSLSRLKEIDMVFGGRIKTLMSNGANIDDSIRDADLVIGAVL
VPGAAAPKLVTRAMLKTMKPGAVLVDVAIDQGGCFETSRPTTHQDPIYTVDGIVHYCVANMPGGVARTSTQALTNATLPY
TLELANKGWRQALLDNAHLRNGLNVCRGRVTYQAVARDLGHAYVDPIEAIKAAN

Sequences:

>Translated_374_residues
MLIGVPKEIKNHEYRVGLTPSGVRELVANGHKVLVQTHAGLAIGFTDEQYIQAGASIASNAEEVFERADMIVKVKEPQPV
ECRMLRPGQILFTYLHLAPDPEQTKLLIESDSVAIAYETVTDERGGLPLLAPMSEVAGRMAIQAGAHALEKAQGGRGVLL
GGVPGVAPAKVVVIGGGVVGLNAARMAMGAGADVTILDKSLSRLKEIDMVFGGRIKTLMSNGANIDDSIRDADLVIGAVL
VPGAAAPKLVTRAMLKTMKPGAVLVDVAIDQGGCFETSRPTTHQDPIYTVDGIVHYCVANMPGGVARTSTQALTNATLPY
TLELANKGWRQALLDNAHLRNGLNVCRGRVTYQAVARDLGHAYVDPIEAIKAAN
>Mature_374_residues
MLIGVPKEIKNHEYRVGLTPSGVRELVANGHKVLVQTHAGLAIGFTDEQYIQAGASIASNAEEVFERADMIVKVKEPQPV
ECRMLRPGQILFTYLHLAPDPEQTKLLIESDSVAIAYETVTDERGGLPLLAPMSEVAGRMAIQAGAHALEKAQGGRGVLL
GGVPGVAPAKVVVIGGGVVGLNAARMAMGAGADVTILDKSLSRLKEIDMVFGGRIKTLMSNGANIDDSIRDADLVIGAVL
VPGAAAPKLVTRAMLKTMKPGAVLVDVAIDQGGCFETSRPTTHQDPIYTVDGIVHYCVANMPGGVARTSTQALTNATLPY
TLELANKGWRQALLDNAHLRNGLNVCRGRVTYQAVARDLGHAYVDPIEAIKAAN

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=342, Percent_Identity=33.0409356725146, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI122939153, Length=342, Percent_Identity=33.0409356725146, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1787887, Length=381, Percent_Identity=31.758530183727, Blast_Score=131, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17550456, Length=344, Percent_Identity=31.6860465116279, Blast_Score=151, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39503; Mature: 39503

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGVPKEIKNHEYRVGLTPSGVRELVANGHKVLVQTHAGLAIGFTDEQYIQAGASIASN
CCCCCCHHHCCCCEEEECCHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHHHCCHHHCC
AEEVFERADMIVKVKEPQPVECRMLRPGQILFTYLHLAPDPEQTKLLIESDSVAIAYETV
HHHHHHHCCEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCEEEEECCCEEEEEEEE
TDERGGLPLLAPMSEVAGRMAIQAGAHALEKAQGGRGVLLGGVPGVAPAKVVVIGGGVVG
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCEEC
LNAARMAMGAGADVTILDKSLSRLKEIDMVFGGRIKTLMSNGANIDDSIRDADLVIGAVL
CCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHEEEHHC
VPGAAAPKLVTRAMLKTMKPGAVLVDVAIDQGGCFETSRPTTHQDPIYTVDGIVHYCVAN
CCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHC
MPGGVARTSTQALTNATLPYTLELANKGWRQALLDNAHLRNGLNVCRGRVTYQAVARDLG
CCCCCHHCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHC
HAYVDPIEAIKAAN
HHHHCHHHHHHCCC
>Mature Secondary Structure
MLIGVPKEIKNHEYRVGLTPSGVRELVANGHKVLVQTHAGLAIGFTDEQYIQAGASIASN
CCCCCCHHHCCCCEEEECCHHHHHHHHHCCCEEEEEECCCEEEECCCHHHHHHCCHHHCC
AEEVFERADMIVKVKEPQPVECRMLRPGQILFTYLHLAPDPEQTKLLIESDSVAIAYETV
HHHHHHHCCEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCEEEEECCCEEEEEEEE
TDERGGLPLLAPMSEVAGRMAIQAGAHALEKAQGGRGVLLGGVPGVAPAKVVVIGGGVVG
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCEEC
LNAARMAMGAGADVTILDKSLSRLKEIDMVFGGRIKTLMSNGANIDDSIRDADLVIGAVL
CCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHEEEHHC
VPGAAAPKLVTRAMLKTMKPGAVLVDVAIDQGGCFETSRPTTHQDPIYTVDGIVHYCVAN
CCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHC
MPGGVARTSTQALTNATLPYTLELANKGWRQALLDNAHLRNGLNVCRGRVTYQAVARDLG
CCCCCHHCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHC
HAYVDPIEAIKAAN
HHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]