Click here to switch to the map view.

The map label for this gene is nolF [H]

Identifier: 34497430

GI number: 34497430

Start: 2148098

End: 2149228

Strand: Direct

Name: nolF [H]

Synonym: CV_1975

Alternate gene names: 34497430

Gene position: 2148098-2149228 (Clockwise)

Preceding gene: 34497429

Following gene: 34497431

Centisome position: 45.21

GC content: 67.11

Gene sequence:

>1131_bases
ATGGCCACATCGGAAAACACACATATGAACATACGTCTTTGCCTGTCCGCCCTGTTTCTTCTATCGCTAGCCGCCTGTGG
CAAGGGCGGCGCCGAGCATGCGTCCGCTTCCGGCAAGAACGCGTCGGCGCCGGCGGCGCGCCGCTTGTCCGGCGCCGATG
TGGTGCGGGCGGAAGTGAGGCCGCTCGCCGCCGCCATTCCGTTCACCGGCTCGCTGAACGCGCTGACCAGCAGCGCGGTG
GCGTCCGAGGTCGACGCCAACGTTCGCGAAGTGAAGGTGCGCGAAGGGGAGAGTGTGCGTCGCGGCCAGTTGCTGGCGGT
GCTGGACGCCGAGTCGTTGGGGCAGTCGGTGGCGGAGCAGAACGCCCAGCTCGACAACTCCAAGGCGCGGCTGAAGCTGG
CCAAGGTCAAGCTGGACAAGCAGCGCGAGCTGTTGGGCAAGGGTTTCATTTCCCAGGTGGCGTTCGACGAGCTGGAAAGC
GATTACCGAGTGAAAGAAGGCGAGCTTCGCGCGCAGTCGGCCCAGTTGGCGCGGGCTCAGCGCTCGCTGGCCGATGCCCA
GGTGAAATCGCCGATAGACGGCGTGGTCTACGAGCGCAAGATCAATCCGGGCGAAGTGGCCAGCCGCAATATGAAGCTGT
TTTCGGTGGCCGACCTGGCGGTGCTGGAGATCGCCGCCACCGTGCCCTCGAGACTGGTGGGGCAGGTCAGGGTGGGCATG
CCGGCTTCGTTCAGCGTCGACGGCCGCGACGTCCAAGAGCATGGCGAAGTGGTGCGCATCAATCCGGTGGCCATCCCCGG
CACCCGCAGCTTCACGCTGTTCGTGCGGGTGCGGAACACGGACGGGCACTTGAAGGTGGGCCAGTTCGCCAAGGGCGGGG
TGGTGGTGCGCGAGGTGAAGGACAAGGTGGTGGTGCCCATCGCCGCGGTGCGCGACATCGACGCCAAGCCCTGGGTGATG
GTGGTGGAGAAGGGCCGGTTGGCGAAGCGGCCGGTAACCGTGGAGTTGCGCGCGGAAAACGAGCGCCAGGCCGCGATTGG
TGGCGTGGCCGCCGGCGCCAGCGTGATCGTCGGCGAACTGCTGGGCAGCAAGGCGGGCGACCCGGTCAGCCTGCCGGCCC
GACTGGGTTGA

Upstream 100 bases:

>100_bases
CAATGACATTAAAATAAATTCACGGATGTGGCATGATGTCGGACAGGCGGCCAAACGGCCGCCTGTTCCGTAATCGACAA
CAAGACCGGATGACGCCGCG

Downstream 100 bases:

>100_bases
GGAGAACGTCATGTGGCTGACCCGCGTAAGCGTGCGCAACCCGTATTTCGCCGCAGTATTGATGATGACGCTGATGGTGC
TGGGCCTGTTCTCCTGGCAG

Product: NolF secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MATSENTHMNIRLCLSALFLLSLAACGKGGAEHASASGKNASAPAARRLSGADVVRAEVRPLAAAIPFTGSLNALTSSAV
ASEVDANVREVKVREGESVRRGQLLAVLDAESLGQSVAEQNAQLDNSKARLKLAKVKLDKQRELLGKGFISQVAFDELES
DYRVKEGELRAQSAQLARAQRSLADAQVKSPIDGVVYERKINPGEVASRNMKLFSVADLAVLEIAATVPSRLVGQVRVGM
PASFSVDGRDVQEHGEVVRINPVAIPGTRSFTLFVRVRNTDGHLKVGQFAKGGVVVREVKDKVVVPIAAVRDIDAKPWVM
VVEKGRLAKRPVTVELRAENERQAAIGGVAAGASVIVGELLGSKAGDPVSLPARLG

Sequences:

>Translated_376_residues
MATSENTHMNIRLCLSALFLLSLAACGKGGAEHASASGKNASAPAARRLSGADVVRAEVRPLAAAIPFTGSLNALTSSAV
ASEVDANVREVKVREGESVRRGQLLAVLDAESLGQSVAEQNAQLDNSKARLKLAKVKLDKQRELLGKGFISQVAFDELES
DYRVKEGELRAQSAQLARAQRSLADAQVKSPIDGVVYERKINPGEVASRNMKLFSVADLAVLEIAATVPSRLVGQVRVGM
PASFSVDGRDVQEHGEVVRINPVAIPGTRSFTLFVRVRNTDGHLKVGQFAKGGVVVREVKDKVVVPIAAVRDIDAKPWVM
VVEKGRLAKRPVTVELRAENERQAAIGGVAAGASVIVGELLGSKAGDPVSLPARLG
>Mature_375_residues
ATSENTHMNIRLCLSALFLLSLAACGKGGAEHASASGKNASAPAARRLSGADVVRAEVRPLAAAIPFTGSLNALTSSAVA
SEVDANVREVKVREGESVRRGQLLAVLDAESLGQSVAEQNAQLDNSKARLKLAKVKLDKQRELLGKGFISQVAFDELESD
YRVKEGELRAQSAQLARAQRSLADAQVKSPIDGVVYERKINPGEVASRNMKLFSVADLAVLEIAATVPSRLVGQVRVGMP
ASFSVDGRDVQEHGEVVRINPVAIPGTRSFTLFVRVRNTDGHLKVGQFAKGGVVVREVKDKVVVPIAAVRDIDAKPWVMV
VEKGRLAKRPVTVELRAENERQAAIGGVAAGASVIVGELLGSKAGDPVSLPARLG

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=330, Percent_Identity=26.6666666666667, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082036, Length=299, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081951, Length=179, Percent_Identity=25.1396648044693, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39767; Mature: 39636

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSENTHMNIRLCLSALFLLSLAACGKGGAEHASASGKNASAPAARRLSGADVVRAEVR
CCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
PLAAAIPFTGSLNALTSSAVASEVDANVREVKVREGESVRRGQLLAVLDAESLGQSVAEQ
HHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHHHHHHHH
NAQLDNSKARLKLAKVKLDKQRELLGKGFISQVAFDELESDYRVKEGELRAQSAQLARAQ
HCCCCCCHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
RSLADAQVKSPIDGVVYERKINPGEVASRNMKLFSVADLAVLEIAATVPSRLVGQVRVGM
HHHHHHHHCCCCCCEEEEECCCCHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHEEECC
PASFSVDGRDVQEHGEVVRINPVAIPGTRSFTLFVRVRNTDGHLKVGQFAKGGVVVREVK
CCEECCCCCCHHHCCCEEEEEEEECCCCCEEEEEEEEECCCCCEEECCCCCCCEEEEECC
DKVVVPIAAVRDIDAKPWVMVVEKGRLAKRPVTVELRAENERQAAIGGVAAGASVIVGEL
CCEEEEEHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHH
LGSKAGDPVSLPARLG
HCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATSENTHMNIRLCLSALFLLSLAACGKGGAEHASASGKNASAPAARRLSGADVVRAEVR
CCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
PLAAAIPFTGSLNALTSSAVASEVDANVREVKVREGESVRRGQLLAVLDAESLGQSVAEQ
HHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHHHHHHHH
NAQLDNSKARLKLAKVKLDKQRELLGKGFISQVAFDELESDYRVKEGELRAQSAQLARAQ
HCCCCCCHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
RSLADAQVKSPIDGVVYERKINPGEVASRNMKLFSVADLAVLEIAATVPSRLVGQVRVGM
HHHHHHHHCCCCCCEEEEECCCCHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHEEECC
PASFSVDGRDVQEHGEVVRINPVAIPGTRSFTLFVRVRNTDGHLKVGQFAKGGVVVREVK
CCEECCCCCCHHHCCCEEEEEEEECCCCCEEEEEEEEECCCCCEEECCCCCCCEEEEECC
DKVVVPIAAVRDIDAKPWVMVVEKGRLAKRPVTVELRAENERQAAIGGVAAGASVIVGEL
CCEEEEEHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHH
LGSKAGDPVSLPARLG
HCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]