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The map label for this gene is tag [H]

Identifier: 34497400

GI number: 34497400

Start: 2118007

End: 2118576

Strand: Reverse

Name: tag [H]

Synonym: CV_1945

Alternate gene names: 34497400

Gene position: 2118576-2118007 (Counterclockwise)

Preceding gene: 34497401

Following gene: 34497398

Centisome position: 44.59

GC content: 66.84

Gene sequence:

>570_bases
ATGAACGAGATCCAACGCTGCGCCTGGTGCGGCGACGATCCGCTGTACGTCGCCTACCACGATCGGGAATGGGGCCGTCC
CGAGCGCGACGACCAGAAGCTGTTCGAGATGCTGGTGCTCGAGGGCGCGCAGGCCGGCCTGTCCTGGATCACCATTTTGA
GAAAGCGCGAGGGCTACCGCGCCGCCTTCCACGGCTTCGATCCGGCCAAGGTGGCGGCGATGACCGACGACGATGTCGAG
CGGCTGATGCAGGATCCCGGCATCGTCCGCAACCGGCTCAAAATCCAGAGCGCCATCCGCAACGCCAAGGTATTCCTGCG
GATGCAGCGGGAGCACGGCAGCTTCGCCGACTGGCTGTGGGCGCACGTCGACGGCCAGCCCATCCTGCGCCGCCGCGACG
ACGCGCGGGTGCCGGCCAGCACCGAACTGTCCGACCGCATCAGCAAGGCGCTGAAAAAGGCTGGCATGAATTTCGTCGGC
AGCACGGTGGTCTACGCCTACCTGCAGGCCTGCGGCGTGGTCAACGACCACCTGTCCGGCTGCTTCCTGCATCCGGACCC
CGGCGGCTGA

Upstream 100 bases:

>100_bases
CGCTGCAGGCCAATCTGTGCAATCCGCTGGTGATCAGCCTGTCCTCGCGCCGCGGCATCCAGAAGACCGGCCTCAAGATC
GACATCCTGCTGCACACGCC

Downstream 100 bases:

>100_bases
AGCCGGCAGCCCTCCCATGAAAAACGGCCGCTTGCATCAGCGGCCGTTTTTGCGGGCAAGAGGCGGATCAGGCGAAAGCC
AGTTGTCCGTTCCGGGCTTC

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MNEIQRCAWCGDDPLYVAYHDREWGRPERDDQKLFEMLVLEGAQAGLSWITILRKREGYRAAFHGFDPAKVAAMTDDDVE
RLMQDPGIVRNRLKIQSAIRNAKVFLRMQREHGSFADWLWAHVDGQPILRRRDDARVPASTELSDRISKALKKAGMNFVG
STVVYAYLQACGVVNDHLSGCFLHPDPGG

Sequences:

>Translated_189_residues
MNEIQRCAWCGDDPLYVAYHDREWGRPERDDQKLFEMLVLEGAQAGLSWITILRKREGYRAAFHGFDPAKVAAMTDDDVE
RLMQDPGIVRNRLKIQSAIRNAKVFLRMQREHGSFADWLWAHVDGQPILRRRDDARVPASTELSDRISKALKKAGMNFVG
STVVYAYLQACGVVNDHLSGCFLHPDPGG
>Mature_189_residues
MNEIQRCAWCGDDPLYVAYHDREWGRPERDDQKLFEMLVLEGAQAGLSWITILRKREGYRAAFHGFDPAKVAAMTDDDVE
RLMQDPGIVRNRLKIQSAIRNAKVFLRMQREHGSFADWLWAHVDGQPILRRRDDARVPASTELSDRISKALKKAGMNFVG
STVVYAYLQACGVVNDHLSGCFLHPDPGG

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=183, Percent_Identity=56.2841530054645, Blast_Score=233, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21458; Mature: 21458

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIQRCAWCGDDPLYVAYHDREWGRPERDDQKLFEMLVLEGAQAGLSWITILRKREGYR
CCCHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCH
AAFHGFDPAKVAAMTDDDVERLMQDPGIVRNRLKIQSAIRNAKVFLRMQREHGSFADWLW
HHHCCCCHHHEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEHHCCCCHHHHHH
AHVDGQPILRRRDDARVPASTELSDRISKALKKAGMNFVGSTVVYAYLQACGVVNDHLSG
HHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCC
CFLHPDPGG
EEECCCCCC
>Mature Secondary Structure
MNEIQRCAWCGDDPLYVAYHDREWGRPERDDQKLFEMLVLEGAQAGLSWITILRKREGYR
CCCHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCH
AAFHGFDPAKVAAMTDDDVERLMQDPGIVRNRLKIQSAIRNAKVFLRMQREHGSFADWLW
HHHCCCCHHHEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEHHCCCCHHHHHH
AHVDGQPILRRRDDARVPASTELSDRISKALKKAGMNFVGSTVVYAYLQACGVVNDHLSG
HHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCC
CFLHPDPGG
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]