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The map label for this gene is phnH [H]

Identifier: 34497300

GI number: 34497300

Start: 1999952

End: 2000524

Strand: Reverse

Name: phnH [H]

Synonym: CV_1845

Alternate gene names: 34497300

Gene position: 2000524-1999952 (Counterclockwise)

Preceding gene: 34497301

Following gene: 34497299

Centisome position: 42.11

GC content: 70.33

Gene sequence:

>573_bases
ATGCTGACAGGTTTTTCCCAGCCAGTGGAAGCGGCCCAAAGCATTTTCCGCGCAGTCCTAAGCGCGATGGCCGAACCCTT
GCTGCCGCGCGCGCTGCCGGCACTGCCGCCGGCCCTGCCGGGGCTGAAGGCCGCCACCGCCGCGCTGCTCTACACGCTGG
CCGATCAGGACGTGCGCGTCTGGCTGCCGGCGCTGCCGGACGACACCGTCGCCAGCCTGCGCTTCCACACCGGCATGCGC
GTGACCGATCGGCCGGAGGAAGCCGACTTCATCGTGATCGCCGCCGGATCGCCGCTGCCGGACCTGATGCGCCTGAAAGC
CGGCAGCGCCGAATATCCAGACCGCTCCGCCACGCTGTTGCTCGAAGCGGACGGTTTCACTCAGCAGCAGGTCGAAGGCA
GCGGCCCCGGCTTCGCCGCGCCGCGCCGCTTCGGCGCCGACGGACTGCCTGCAAGCTTCTGGCGCGAATGGCAACACAAC
CGCGCCCGCTTCCCGCTCGGCGTGGACGCCGTGCTGATTACCGACACCCACATCGCCGGCCTGCCGCGCAGCGTCCGCCT
CCGGGAGGGTTGA

Upstream 100 bases:

>100_bases
GCTCCGCTGGAAACCGAACTGGCCGAGCGCCGCGCAGCCCGCTCTCGGGAAGCCGCCGCCAGCAAGGTCGAGTTTTTCAC
CATGGTCCGGGGGGCGTGAG

Downstream 100 bases:

>100_bases
GATGTATGTAGCCGTCAAAGGCGGGGAAACCGCCATCGCCCGCTCCTGGGACCTGCTGGCCGCCGCCCGCCGCGGCGACG
AGGCCGCGCCGGAGCTGAGC

Product: phosphonate metabolism protein PhnH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MLTGFSQPVEAAQSIFRAVLSAMAEPLLPRALPALPPALPGLKAATAALLYTLADQDVRVWLPALPDDTVASLRFHTGMR
VTDRPEEADFIVIAAGSPLPDLMRLKAGSAEYPDRSATLLLEADGFTQQQVEGSGPGFAAPRRFGADGLPASFWREWQHN
RARFPLGVDAVLITDTHIAGLPRSVRLREG

Sequences:

>Translated_190_residues
MLTGFSQPVEAAQSIFRAVLSAMAEPLLPRALPALPPALPGLKAATAALLYTLADQDVRVWLPALPDDTVASLRFHTGMR
VTDRPEEADFIVIAAGSPLPDLMRLKAGSAEYPDRSATLLLEADGFTQQQVEGSGPGFAAPRRFGADGLPASFWREWQHN
RARFPLGVDAVLITDTHIAGLPRSVRLREG
>Mature_190_residues
MLTGFSQPVEAAQSIFRAVLSAMAEPLLPRALPALPPALPGLKAATAALLYTLADQDVRVWLPALPDDTVASLRFHTGMR
VTDRPEEADFIVIAAGSPLPDLMRLKAGSAEYPDRSATLLLEADGFTQQQVEGSGPGFAAPRRFGADGLPASFWREWQHN
RARFPLGVDAVLITDTHIAGLPRSVRLREG

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3625

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnH family [H]

Homologues:

Organism=Escherichia coli, GI1790538, Length=189, Percent_Identity=35.4497354497354, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008772 [H]

Pfam domain/function: PF05845 PhnH [H]

EC number: NA

Molecular weight: Translated: 20449; Mature: 20449

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGFSQPVEAAQSIFRAVLSAMAEPLLPRALPALPPALPGLKAATAALLYTLADQDVRV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEE
WLPALPDDTVASLRFHTGMRVTDRPEEADFIVIAAGSPLPDLMRLKAGSAEYPDRSATLL
EECCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCEEEE
LEADGFTQQQVEGSGPGFAAPRRFGADGLPASFWREWQHNRARFPLGVDAVLITDTHIAG
EECCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCHHHC
LPRSVRLREG
CCCCEEECCC
>Mature Secondary Structure
MLTGFSQPVEAAQSIFRAVLSAMAEPLLPRALPALPPALPGLKAATAALLYTLADQDVRV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEE
WLPALPDDTVASLRFHTGMRVTDRPEEADFIVIAAGSPLPDLMRLKAGSAEYPDRSATLL
EECCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCEEEE
LEADGFTQQQVEGSGPGFAAPRRFGADGLPASFWREWQHNRARFPLGVDAVLITDTHIAG
EECCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCHHHC
LPRSVRLREG
CCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]