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The map label for this gene is phnI [H]

Identifier: 34497299

GI number: 34497299

Start: 1998865

End: 1999950

Strand: Reverse

Name: phnI [H]

Synonym: CV_1844

Alternate gene names: 34497299

Gene position: 1999950-1998865 (Counterclockwise)

Preceding gene: 34497300

Following gene: 34497298

Centisome position: 42.09

GC content: 69.43

Gene sequence:

>1086_bases
ATGTATGTAGCCGTCAAAGGCGGGGAAACCGCCATCGCCCGCTCCTGGGACCTGCTGGCCGCCGCCCGCCGCGGCGACGA
GGCCGCGCCGGAGCTGAGCATCGCTCAAATCCGCCAGCAGCTGCGCCTGGCCGTGGCCCGCGTGATGCAGGAAGGCTCGC
TGTACGATGAAGAGCTGGCCGCGCTGGCGCTGAAACAGGCCAGCGGCGACAGCATCGAAGCCGTATTCCTGCTGCGCGCC
TTCCGCACCACGCTGCCGCGCTTCGGCCAGACGCGGCCGCTTAACACCGCCGCGATGCGGGTGCGCCGCCGCGTGTCCGG
CACCTTCAAGGACGTGCCGGGCGGGCAGATTCTCGGCCCCACCGCCGATTACACCCAGCGCCTGCTGGATTTCAGCCTGA
TGGAAGCGGCGCAAAAGATGGAACTGGAACAGGCCGCCGCGCCGCTGCCGGATGCGCTGCCCGGCGTGCTGCGGCTGCTG
GACCGCGAGGGCTTGATCGAGGCCGACCTGCCGCCCAACGGCGATCCGGAGCCGGTGGACCTGACCCGCGAGCCGCTGAT
GTTCCCCGCCGGCCGCCCGGCGCGGCTGCAGAACTTGGCGCGCGGCGACGAGGGCTGGCTATTGGGCCTCGCCTACTCCA
CCCAGCGCGGCTACGCCGAGAGCCACCCCTTCGCCGGCGAGATCCGCATGGGCGAAGTCAGCGTGATGGTTTGCCCGGAA
GAGCTGGGCTTCGAGATCGACATCGGCGAGATCACGCTGACCGAATGCCAGATGATCAATCAGTTCGCCGGCAGCAAGAC
CGCGCCGCCGCAATTCACCCGCGGCTACGGCCTGAGCTTCGGCGAGGCCGAGCGCAAGGCGATGGCGATGGCGCTGGTGG
ACCGCAGCCTGCGCGCCGAGGAGCTGGGCGAAACCGCCCACGCCCCGGCGCAGCAGGCCGAGTTCGCGCTGTGCCACAGC
GACAATGTCGAGGCCTCCGGCTTCGTTCAACACCTGAAACTGCCGCATTACGTGGACTTCCAGGCCGAGCTGGCGCTGAT
CCGCCAGCTGCGCGCCGACGCCGCCCAGGAGAAGACTGATGACTAA

Upstream 100 bases:

>100_bases
CACAACCGCGCCCGCTTCCCGCTCGGCGTGGACGCCGTGCTGATTACCGACACCCACATCGCCGGCCTGCCGCGCAGCGT
CCGCCTCCGGGAGGGTTGAG

Downstream 100 bases:

>100_bases
GGCCCGCGACGATCGCTACAACTTCGGCTTCCTCGACGAGAACACCAAGCGCATGCTGCGCCGCAGCCTGCTCAAGGCGG
TGGCGATCCCCGGCTACCAG

Product: phosphonate metabolism protein PhnI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MYVAVKGGETAIARSWDLLAAARRGDEAAPELSIAQIRQQLRLAVARVMQEGSLYDEELAALALKQASGDSIEAVFLLRA
FRTTLPRFGQTRPLNTAAMRVRRRVSGTFKDVPGGQILGPTADYTQRLLDFSLMEAAQKMELEQAAAPLPDALPGVLRLL
DREGLIEADLPPNGDPEPVDLTREPLMFPAGRPARLQNLARGDEGWLLGLAYSTQRGYAESHPFAGEIRMGEVSVMVCPE
ELGFEIDIGEITLTECQMINQFAGSKTAPPQFTRGYGLSFGEAERKAMAMALVDRSLRAEELGETAHAPAQQAEFALCHS
DNVEASGFVQHLKLPHYVDFQAELALIRQLRADAAQEKTDD

Sequences:

>Translated_361_residues
MYVAVKGGETAIARSWDLLAAARRGDEAAPELSIAQIRQQLRLAVARVMQEGSLYDEELAALALKQASGDSIEAVFLLRA
FRTTLPRFGQTRPLNTAAMRVRRRVSGTFKDVPGGQILGPTADYTQRLLDFSLMEAAQKMELEQAAAPLPDALPGVLRLL
DREGLIEADLPPNGDPEPVDLTREPLMFPAGRPARLQNLARGDEGWLLGLAYSTQRGYAESHPFAGEIRMGEVSVMVCPE
ELGFEIDIGEITLTECQMINQFAGSKTAPPQFTRGYGLSFGEAERKAMAMALVDRSLRAEELGETAHAPAQQAEFALCHS
DNVEASGFVQHLKLPHYVDFQAELALIRQLRADAAQEKTDD
>Mature_361_residues
MYVAVKGGETAIARSWDLLAAARRGDEAAPELSIAQIRQQLRLAVARVMQEGSLYDEELAALALKQASGDSIEAVFLLRA
FRTTLPRFGQTRPLNTAAMRVRRRVSGTFKDVPGGQILGPTADYTQRLLDFSLMEAAQKMELEQAAAPLPDALPGVLRLL
DREGLIEADLPPNGDPEPVDLTREPLMFPAGRPARLQNLARGDEGWLLGLAYSTQRGYAESHPFAGEIRMGEVSVMVCPE
ELGFEIDIGEITLTECQMINQFAGSKTAPPQFTRGYGLSFGEAERKAMAMALVDRSLRAEELGETAHAPAQQAEFALCHS
DNVEASGFVQHLKLPHYVDFQAELALIRQLRADAAQEKTDD

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=351, Percent_Identity=58.974358974359, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 39489; Mature: 39489

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGETAIARSWDLLAAARRGDEAAPELSIAQIRQQLRLAVARVMQEGSLYDEELA
CEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ALALKQASGDSIEAVFLLRAFRTTLPRFGQTRPLNTAAMRVRRRVSGTFKDVPGGQILGP
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECC
TADYTQRLLDFSLMEAAQKMELEQAAAPLPDALPGVLRLLDREGLIEADLPPNGDPEPVD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCC
LTREPLMFPAGRPARLQNLARGDEGWLLGLAYSTQRGYAESHPFAGEIRMGEVSVMVCPE
CCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEECHH
ELGFEIDIGEITLTECQMINQFAGSKTAPPQFTRGYGLSFGEAERKAMAMALVDRSLRAE
HHCCEEEECCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELGETAHAPAQQAEFALCHSDNVEASGFVQHLKLPHYVDFQAELALIRQLRADAAQEKTD
HHCHHHCCCHHHHCEEEECCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHCCCC
D
C
>Mature Secondary Structure
MYVAVKGGETAIARSWDLLAAARRGDEAAPELSIAQIRQQLRLAVARVMQEGSLYDEELA
CEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ALALKQASGDSIEAVFLLRAFRTTLPRFGQTRPLNTAAMRVRRRVSGTFKDVPGGQILGP
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECC
TADYTQRLLDFSLMEAAQKMELEQAAAPLPDALPGVLRLLDREGLIEADLPPNGDPEPVD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCC
LTREPLMFPAGRPARLQNLARGDEGWLLGLAYSTQRGYAESHPFAGEIRMGEVSVMVCPE
CCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCEEEEECHH
ELGFEIDIGEITLTECQMINQFAGSKTAPPQFTRGYGLSFGEAERKAMAMALVDRSLRAE
HHCCEEEECCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELGETAHAPAQQAEFALCHSDNVEASGFVQHLKLPHYVDFQAELALIRQLRADAAQEKTD
HHCHHHCCCHHHHCEEEECCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]