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The map label for this gene is phnJ [H]

Identifier: 34497298

GI number: 34497298

Start: 1998030

End: 1998872

Strand: Reverse

Name: phnJ [H]

Synonym: CV_1843

Alternate gene names: 34497298

Gene position: 1998872-1998030 (Counterclockwise)

Preceding gene: 34497299

Following gene: 34497297

Centisome position: 42.07

GC content: 64.77

Gene sequence:

>843_bases
ATGACTAAGGCCCGCGACGATCGCTACAACTTCGGCTTCCTCGACGAGAACACCAAGCGCATGCTGCGCCGCAGCCTGCT
CAAGGCGGTGGCGATCCCCGGCTACCAGGTGCCGTTCGGCAGCCGCGAAATGCCGCTGCCCTACGGCTGGGGCACCGGCG
GCATCCAGCTGACGGCCAGCGTGATCGGCCGCGACGACGTACTCAAGGTCATCGACCAGGGCGCCGACGACACCACCAAC
GCCGTCAACATCCGCCGCTTCTTCGGCCGCGTGGCCGGCGTCGCCACCACCACTCGCACCGCCGACGCGACGCTGATCCA
GACCCGCCATCGCATCCCGGAAACGCCGCTGAAACCCGGCCAAATCCTGATCTTCCAGGTGCCGGAGCCGGAACCGCTGC
GCACGCTGGAGGCCAGCGAGGTGGAAACCCGCAAGCTGCACGCGCACCAGGAATACGGCCTGATGCACGTCAAGCTGTAC
GAGGACATCGCCCGCTTCGGCCACATCGCCACCAGCTACGACTACCCGGTGATGGTGAACGACAGGTATCTGGCCTCGCC
GTCGCCGATTCCGAAATTCGACAACCCCAAGCTCGACATGAACCCGGCCCTGCTGCTGTTCGGCGCCGGCCGCGAAAAGC
GGCTGTACGCGATTCCGCCCTACACCAAGGTGGAAAGCCTGGCCTTCGACGACCACCCGTTCGAAATCCAGAGCTGGCAG
CAAGGCTGCGCGATTTGCGGTTCGCACGACAGCTATCTGGACGAAATCGTCATCGACGACGCCGGCAGCCGGATGTTTGT
CTGCTCGGACACCGACTACTGCCAGCGGGAGAAACAAGCATGA

Upstream 100 bases:

>100_bases
GGCTTCGTTCAACACCTGAAACTGCCGCATTACGTGGACTTCCAGGCCGAGCTGGCGCTGATCCGCCAGCTGCGCGCCGA
CGCCGCCCAGGAGAAGACTG

Downstream 100 bases:

>100_bases
TGGCGCCCATCCTCACCGTGAGCGGGCTGAGCAAGGACTACGGCAACGGCCAGGGCTGCTTCGACGTCAATTTCCAGCTG
TATCCGGGCGAGGTGCTGTG

Product: phosphonate metabolism protein PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTKARDDRYNFGFLDENTKRMLRRSLLKAVAIPGYQVPFGSREMPLPYGWGTGGIQLTASVIGRDDVLKVIDQGADDTTN
AVNIRRFFGRVAGVATTTRTADATLIQTRHRIPETPLKPGQILIFQVPEPEPLRTLEASEVETRKLHAHQEYGLMHVKLY
EDIARFGHIATSYDYPVMVNDRYLASPSPIPKFDNPKLDMNPALLLFGAGREKRLYAIPPYTKVESLAFDDHPFEIQSWQ
QGCAICGSHDSYLDEIVIDDAGSRMFVCSDTDYCQREKQA

Sequences:

>Translated_280_residues
MTKARDDRYNFGFLDENTKRMLRRSLLKAVAIPGYQVPFGSREMPLPYGWGTGGIQLTASVIGRDDVLKVIDQGADDTTN
AVNIRRFFGRVAGVATTTRTADATLIQTRHRIPETPLKPGQILIFQVPEPEPLRTLEASEVETRKLHAHQEYGLMHVKLY
EDIARFGHIATSYDYPVMVNDRYLASPSPIPKFDNPKLDMNPALLLFGAGREKRLYAIPPYTKVESLAFDDHPFEIQSWQ
QGCAICGSHDSYLDEIVIDDAGSRMFVCSDTDYCQREKQA
>Mature_279_residues
TKARDDRYNFGFLDENTKRMLRRSLLKAVAIPGYQVPFGSREMPLPYGWGTGGIQLTASVIGRDDVLKVIDQGADDTTNA
VNIRRFFGRVAGVATTTRTADATLIQTRHRIPETPLKPGQILIFQVPEPEPLRTLEASEVETRKLHAHQEYGLMHVKLYE
DIARFGHIATSYDYPVMVNDRYLASPSPIPKFDNPKLDMNPALLLFGAGREKRLYAIPPYTKVESLAFDDHPFEIQSWQQ
GCAICGSHDSYLDEIVIDDAGSRMFVCSDTDYCQREKQA

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=272, Percent_Identity=76.1029411764706, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 31586; Mature: 31455

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKARDDRYNFGFLDENTKRMLRRSLLKAVAIPGYQVPFGSREMPLPYGWGTGGIQLTAS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEE
VIGRDDVLKVIDQGADDTTNAVNIRRFFGRVAGVATTTRTADATLIQTRHRIPETPLKPG
HHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCC
QILIFQVPEPEPLRTLEASEVETRKLHAHQEYGLMHVKLYEDIARFGHIATSYDYPVMVN
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEHHHHHHHHHCCHHCCCCCCEEEC
DRYLASPSPIPKFDNPKLDMNPALLLFGAGREKRLYAIPPYTKVESLAFDDHPFEIQSWQ
CCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHEECCCCCCCHHCHH
QGCAICGSHDSYLDEIVIDDAGSRMFVCSDTDYCQREKQA
CCCEECCCCHHHHHHEEEECCCCEEEEECCCHHHHHHCCC
>Mature Secondary Structure 
TKARDDRYNFGFLDENTKRMLRRSLLKAVAIPGYQVPFGSREMPLPYGWGTGGIQLTAS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEE
VIGRDDVLKVIDQGADDTTNAVNIRRFFGRVAGVATTTRTADATLIQTRHRIPETPLKPG
HHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCC
QILIFQVPEPEPLRTLEASEVETRKLHAHQEYGLMHVKLYEDIARFGHIATSYDYPVMVN
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEHHHHHHHHHCCHHCCCCCCEEEC
DRYLASPSPIPKFDNPKLDMNPALLLFGAGREKRLYAIPPYTKVESLAFDDHPFEIQSWQ
CCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHEECCCCCCCHHCHH
QGCAICGSHDSYLDEIVIDDAGSRMFVCSDTDYCQREKQA
CCCEECCCCHHHHHHEEEECCCCEEEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]