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The map label for this gene is ppaX [H]

Identifier: 34497272

GI number: 34497272

Start: 1973686

End: 1974342

Strand: Reverse

Name: ppaX [H]

Synonym: CV_1817

Alternate gene names: 34497272

Gene position: 1974342-1973686 (Counterclockwise)

Preceding gene: 34497273

Following gene: 34497271

Centisome position: 41.56

GC content: 69.25

Gene sequence:

>657_bases
ATGCGTGAATATGATCTGGTAGTGTTCGACTGGGACGGCACGCTGATGGACTCCACCGCCCATATCGTGAACGCCATCCA
GCAAGCCTGCCGCGATCTGGCCCTGCCGGTGCCTGGCCGCGAGGCCGCCAGCCACGTGATCGGACTCAGGCTGGCCGAGG
CGATGCAACAGTTCTGCCCGCCGGACCAGGCGACGCGGCTGCCGGACCTGGTAGACGCCTTTCGCCGCCGCTACCAGGCC
GATCTCGACCGCGTCACGCTGTTCGACGCCGCCCGCGACACGCTGGAGGCGATACGCGACGCCGGCGCCTTCGTCGCCAT
CGCCACCGGCAGCAGCCGCGCCGGCCTGGACCGCGCATTGGCCGCGGCCGGCATAGGCGAGCTGTTCGACGCCACCCGCA
CCGTGGACGAATGCCACTCCAAGCCGCACCCGGACATGCTGTTGCAGCTGACCGCTTATTTCGGCGTCGAATGCCGCCGC
GCGGTGATGGTGGGAGACACCAGCCACGACCTGCTGATGGCGCTGAATGCGGGCAGCCACGGCGTCGGCATCAGTCACGG
CGCCCACGGCAGCGAGGAGCTGACGCGCTGCCAGCCCCGCGCCATCGTCCACTCGCTGCCCGAGCTGTCGTCATGGCTGC
TCCCCAGGCTCTGCTGA

Upstream 100 bases:

>100_bases
GCCTGACCCTGCCGCATCCGCTGACCGGCGCCACGCTGAAACTGGAGGCGCCGCTGCCGCCGGAGCTGCAGAAATTCCTG
AAAAGCCTGGAGCCGCGCTG

Downstream 100 bases:

>100_bases
TCTGCGGCTCCGCCGAACTGGAAGACGGCGGCCTCGGCCGCCGCTTCGAGATCGAGCAAACCGATGGCGGCCGCCTGCCC
GCCTTCGTCATCCGCTACCG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MREYDLVVFDWDGTLMDSTAHIVNAIQQACRDLALPVPGREAASHVIGLRLAEAMQQFCPPDQATRLPDLVDAFRRRYQA
DLDRVTLFDAARDTLEAIRDAGAFVAIATGSSRAGLDRALAAAGIGELFDATRTVDECHSKPHPDMLLQLTAYFGVECRR
AVMVGDTSHDLLMALNAGSHGVGISHGAHGSEELTRCQPRAIVHSLPELSSWLLPRLC

Sequences:

>Translated_218_residues
MREYDLVVFDWDGTLMDSTAHIVNAIQQACRDLALPVPGREAASHVIGLRLAEAMQQFCPPDQATRLPDLVDAFRRRYQA
DLDRVTLFDAARDTLEAIRDAGAFVAIATGSSRAGLDRALAAAGIGELFDATRTVDECHSKPHPDMLLQLTAYFGVECRR
AVMVGDTSHDLLMALNAGSHGVGISHGAHGSEELTRCQPRAIVHSLPELSSWLLPRLC
>Mature_218_residues
MREYDLVVFDWDGTLMDSTAHIVNAIQQACRDLALPVPGREAASHVIGLRLAEAMQQFCPPDQATRLPDLVDAFRRRYQA
DLDRVTLFDAARDTLEAIRDAGAFVAIATGSSRAGLDRALAAAGIGELFDATRTVDECHSKPHPDMLLQLTAYFGVECRR
AVMVGDTSHDLLMALNAGSHGVGISHGAHGSEELTRCQPRAIVHSLPELSSWLLPRLC

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=228, Percent_Identity=28.9473684210526, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 23729; Mature: 23729

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREYDLVVFDWDGTLMDSTAHIVNAIQQACRDLALPVPGREAASHVIGLRLAEAMQQFCP
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PDQATRLPDLVDAFRRRYQADLDRVTLFDAARDTLEAIRDAGAFVAIATGSSRAGLDRAL
CCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AAAGIGELFDATRTVDECHSKPHPDMLLQLTAYFGVECRRAVMVGDTSHDLLMALNAGSH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHEEEECCCCCCEEEEECCCCC
GVGISHGAHGSEELTRCQPRAIVHSLPELSSWLLPRLC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MREYDLVVFDWDGTLMDSTAHIVNAIQQACRDLALPVPGREAASHVIGLRLAEAMQQFCP
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PDQATRLPDLVDAFRRRYQADLDRVTLFDAARDTLEAIRDAGAFVAIATGSSRAGLDRAL
CCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AAAGIGELFDATRTVDECHSKPHPDMLLQLTAYFGVECRRAVMVGDTSHDLLMALNAGSH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHEEEECCCCCCEEEEECCCCC
GVGISHGAHGSEELTRCQPRAIVHSLPELSSWLLPRLC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9465101; 12359880 [H]