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The map label for this gene is pfkA [H]

Identifier: 34497200

GI number: 34497200

Start: 1895912

End: 1897129

Strand: Direct

Name: pfkA [H]

Synonym: CV_1745

Alternate gene names: 34497200

Gene position: 1895912-1897129 (Clockwise)

Preceding gene: 34497199

Following gene: 34497201

Centisome position: 39.9

GC content: 68.23

Gene sequence:

>1218_bases
ATGACCAAGCCAAGAGCCGTATATCTGCAATCCGGCGGACCCACCGCCGTTCTGAACGCTTCCGCCCAGGGCGCGATTGA
AACCGCGCGTCGCCTCGGCCTTTCCTTGTACGCCGCTTACGATGGCCTGGCGGGATTGCTGGATGGCCGCCTGTGCGATA
CCGACGGCGTGTCCGACACCGCCATCGCCCAGCTGGCCTTCATGCCCGGCGGCAGCTTCGGCGTCAGCCGCCGCATGATA
GGCACGTTCGAGGAAGCGCCGGAAGACTGGCTGCGGCTGCGCGACGTGCTGGCCAAGCACGACATCCACTACCTGCTGAT
CAACGGCGGCAACGGTTCGCTGGGCTGCGCCGAGCGCCTGGTCGATTTCGAAAAGCACACCGGCTACAAGCTGGGCGTGA
TCGGCATCCCCAAGACCATAGACAACGACCTGGTCGGCACCGACAACAGCCCCGGCTACGGCTCCTCCGCCAAATTCCTG
GCCAGCGCGATGCGCGAGGTCGGCCTGGACATGACCAGTATGGGCTGGGGCCGGGTGTTCATCATGGAAACCATGGGCCG
CCACACCGGCTGGCTGGCGGCCGCCTGCGCCGCCGCCTCCCGCAATCCGCACGAAGCGCCGCATTTGCTGCTGTTGCCCG
AGGTGCCGTTCGACCAGGCGCGCTTCCTGGCGGCGCTGGACGCCAGCCTGGCGAAGTACGGCCAGTGCGCGATCGCGGTG
GCCGAAGGCATCACCGGCCAGGACGGCCGCTTCGTCGCCGAGGCCAAGAAGTCCGAAACCTACGGCCACGAGCAACTGGG
CGGCGCCGGCCACTGGCTGGCCCAGCTGATCCTGCGCGAGCGCGGCATCTCCGCCCACGTGGCGCAGGTGGACTATCTGC
AGCGCGCCGGCGGCCACCTGGCTTCCGCCACCGACGTCCGCCAGGCCTACGTGATGGGCGAGAGGGCGGTGGAGTGGCTG
CTGGCCGGCAAGACCGGCCTGATGGCCGGCATCAGGCGGCTGAACGACCATCCTTACTGCTGGGACGTGGCCGAAGTGCC
GCTGTCGGCGGTGGGCGACAAGGAGAAGCGGCTGCCGCCGGAGTTCATCGGCGCCGACGGCTTCAGCGTCACCCAGGCTT
TCCTGGACTACGTGAAGCCGTTGATAGAGGGCGAGCGCATCCCGCCGTTCAAGGACGGCTTGCCGGACTACCGCCCGATC
GCCTGGCCGCGGGTCTGA

Upstream 100 bases:

>100_bases
GAGGCTGTTCTCGGGCCAGTGGTATGGTGGTGGCGTTAGCAGCGTAGGCGTGCTGTCGGCCTTTGCGCTGCCCAGATTGT
CCACTTTTCAGGTTGCGATC

Downstream 100 bases:

>100_bases
GCGGGATCGAACGGCGAAGCGGCCGCCGCGTCGAGAGGCGCGGCGGCCGTTTGCGCATTTCAGCGCGTAATGTCGCGGCT
CGAATGACAGAAGCCGGCGC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTKPRAVYLQSGGPTAVLNASAQGAIETARRLGLSLYAAYDGLAGLLDGRLCDTDGVSDTAIAQLAFMPGGSFGVSRRMI
GTFEEAPEDWLRLRDVLAKHDIHYLLINGGNGSLGCAERLVDFEKHTGYKLGVIGIPKTIDNDLVGTDNSPGYGSSAKFL
ASAMREVGLDMTSMGWGRVFIMETMGRHTGWLAAACAAASRNPHEAPHLLLLPEVPFDQARFLAALDASLAKYGQCAIAV
AEGITGQDGRFVAEAKKSETYGHEQLGGAGHWLAQLILRERGISAHVAQVDYLQRAGGHLASATDVRQAYVMGERAVEWL
LAGKTGLMAGIRRLNDHPYCWDVAEVPLSAVGDKEKRLPPEFIGADGFSVTQAFLDYVKPLIEGERIPPFKDGLPDYRPI
AWPRV

Sequences:

>Translated_405_residues
MTKPRAVYLQSGGPTAVLNASAQGAIETARRLGLSLYAAYDGLAGLLDGRLCDTDGVSDTAIAQLAFMPGGSFGVSRRMI
GTFEEAPEDWLRLRDVLAKHDIHYLLINGGNGSLGCAERLVDFEKHTGYKLGVIGIPKTIDNDLVGTDNSPGYGSSAKFL
ASAMREVGLDMTSMGWGRVFIMETMGRHTGWLAAACAAASRNPHEAPHLLLLPEVPFDQARFLAALDASLAKYGQCAIAV
AEGITGQDGRFVAEAKKSETYGHEQLGGAGHWLAQLILRERGISAHVAQVDYLQRAGGHLASATDVRQAYVMGERAVEWL
LAGKTGLMAGIRRLNDHPYCWDVAEVPLSAVGDKEKRLPPEFIGADGFSVTQAFLDYVKPLIEGERIPPFKDGLPDYRPI
AWPRV
>Mature_404_residues
TKPRAVYLQSGGPTAVLNASAQGAIETARRLGLSLYAAYDGLAGLLDGRLCDTDGVSDTAIAQLAFMPGGSFGVSRRMIG
TFEEAPEDWLRLRDVLAKHDIHYLLINGGNGSLGCAERLVDFEKHTGYKLGVIGIPKTIDNDLVGTDNSPGYGSSAKFLA
SAMREVGLDMTSMGWGRVFIMETMGRHTGWLAAACAAASRNPHEAPHLLLLPEVPFDQARFLAALDASLAKYGQCAIAVA
EGITGQDGRFVAEAKKSETYGHEQLGGAGHWLAQLILRERGISAHVAQVDYLQRAGGHLASATDVRQAYVMGERAVEWLL
AGKTGLMAGIRRLNDHPYCWDVAEVPLSAVGDKEKRLPPEFIGADGFSVTQAFLDYVKPLIEGERIPPFKDGLPDYRPIA
WPRV

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=350, Percent_Identity=26.5714285714286, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI266453619, Length=375, Percent_Identity=25.3333333333333, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI266453768, Length=375, Percent_Identity=25.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI266453748, Length=375, Percent_Identity=25.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4505749, Length=375, Percent_Identity=25.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI48762920, Length=374, Percent_Identity=24.8663101604278, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790350, Length=327, Percent_Identity=29.3577981651376, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=27.741935483871, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321679, Length=332, Percent_Identity=26.5060240963855, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28573326, Length=225, Percent_Identity=26.2222222222222, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17647809, Length=318, Percent_Identity=25.4716981132075, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24652337, Length=318, Percent_Identity=25.4716981132075, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 43619; Mature: 43488

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPRAVYLQSGGPTAVLNASAQGAIETARRLGLSLYAAYDGLAGLLDGRLCDTDGVSDT
CCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
AIAQLAFMPGGSFGVSRRMIGTFEEAPEDWLRLRDVLAKHDIHYLLINGGNGSLGCAERL
HHHHEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHH
VDFEKHTGYKLGVIGIPKTIDNDLVGTDNSPGYGSSAKFLASAMREVGLDMTSMGWGRVF
HHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEE
IMETMGRHTGWLAAACAAASRNPHEAPHLLLLPEVPFDQARFLAALDASLAKYGQCAIAV
EEHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEE
AEGITGQDGRFVAEAKKSETYGHEQLGGAGHWLAQLILRERGISAHVAQVDYLQRAGGHL
ECCCCCCCCCEEEHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
ASATDVRQAYVMGERAVEWLLAGKTGLMAGIRRLNDHPYCWDVAEVPLSAVGDKEKRLPP
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHCCHHHCCCHHHCCCH
EFIGADGFSVTQAFLDYVKPLIEGERIPPFKDGLPDYRPIAWPRV
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKPRAVYLQSGGPTAVLNASAQGAIETARRLGLSLYAAYDGLAGLLDGRLCDTDGVSDT
CCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
AIAQLAFMPGGSFGVSRRMIGTFEEAPEDWLRLRDVLAKHDIHYLLINGGNGSLGCAERL
HHHHEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHH
VDFEKHTGYKLGVIGIPKTIDNDLVGTDNSPGYGSSAKFLASAMREVGLDMTSMGWGRVF
HHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEE
IMETMGRHTGWLAAACAAASRNPHEAPHLLLLPEVPFDQARFLAALDASLAKYGQCAIAV
EEHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEE
AEGITGQDGRFVAEAKKSETYGHEQLGGAGHWLAQLILRERGISAHVAQVDYLQRAGGHL
ECCCCCCCCCEEEHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
ASATDVRQAYVMGERAVEWLLAGKTGLMAGIRRLNDHPYCWDVAEVPLSAVGDKEKRLPP
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHCCHHHCCCHHHCCCH
EFIGADGFSVTQAFLDYVKPLIEGERIPPFKDGLPDYRPIAWPRV
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550409 [H]