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The map label for this gene is luxQ [H]

Identifier: 34497173

GI number: 34497173

Start: 1858361

End: 1860046

Strand: Reverse

Name: luxQ [H]

Synonym: CV_1718

Alternate gene names: 34497173

Gene position: 1860046-1858361 (Counterclockwise)

Preceding gene: 34497174

Following gene: 34497171

Centisome position: 39.15

GC content: 63.88

Gene sequence:

>1686_bases
GTGATCATTTCCTTTACGCTGTACGCGGCGGTGATCAAGCGTCCGATCACCAACAAGGTCTCCGACCTGTACTGGATGGC
GGCGCAGATGCAGCTGACGTTCAACCGCCTGGAAATGGACGTCTGGCGGTACCGCGCCGGCCAGATCACCCGCGAGGAAA
CCGACAAGAGCTACGCCATCGCCTACAGCAAGTACCGGATGTTCACCCGGCCATCGGCCGTCAATACCGACTTGGAGAAG
ATAGAAGGCTTCGACCAGATCAAGGCGCTGCTGGCCAGAATTTTCCAGCATCCGCCGCGCCAGTGGACGCAGCAGGACGC
GCTGGAACTGTCGCGCGACATGGAGGAGATGCGTCCATTGCTGGCCGATTACGGCGTCAAGGCCCGCTACGCGGAAAACA
GCCTGGTGGCGCAACAGCTGCGCATGGTGGAGCGGCACCAGACCATGTACCTGCTGGGCCTGATGTGCTGGCTGGCCTTC
CTCGGCATTTTCTGGTTCGTGCTGCACCGGCTGGCGGAAATCAGGCGACACTCGCTGCAACAGCAACAGGTGCTGGAGAG
GGAGCAGGCCGCCAGGGCCGCGCTGGTGCAAACCGAGCTGGCCCGCGACACCTTCCTCGCCACCATCAGCCACGAAATCC
GCTCACCGCTGCAAAGCATACAGACTTGCACCGAGCTGCTGGAATACAGCATCCCGCCCGACAATCCGGGTTACGGCTAT
CTGTCCCGCCTCAAGCAGAGCAACGCCTATCTGCTGGCCCAGGTGCGGGACATCATGGACATCTCGGCGCTGAAAAACCA
TCAGCTCGCGCTCGAACCCGCCACCACGGACATAGAGGAATTGGTGGCCTGCGTCGTCGCCGTCCACCAGGGCAATGCCG
AGGCCAAGGGACTTGCGCTGACGGTGGCCGTGCCGCCGATGCCGCTGCTGTGGCTGGACGGCAACCGGCTGCGGCAAATC
ATGTGGAACCTGCTGTCCAACGCCATCCGCTACACCGACCAGGGAGGAATCCGCCTGGAACTGGAATATCGGCCGCGCAA
TCTGGTGCTCAGCGTCGCCGACACCGGCGTGGGCATTCCCGAAGACATCAGGGTCCAGTTGTTCCGCCCCTTCACCCGCG
GCAAGACCCGTCGCCCAGGCAGCAGCGGCCTGGGCCTGGCCATCGTGCACGAGCTGGTCACGCTGTTCGGCGGCCAGATC
GAGATCGACAGCCAGGAAGGCCGCGGCAGCACGTTCACGCTGAGCTTGCCGGTCCAGGAAGCGTCCACCCCGGAAGACTG
CGACGGCGCCAGCTACGGCCAAGGCCGCATCCTGCTGCTGGACGACGACGATCCGATACGCGAATCCTACAGCGCGCTGC
TGCAGGAAAACGGCTACAGCGTGTCCAGCTTCGCCACGGTCAACGACGCGGCGGCCCAAGTGGTGCGCCACCGCTTCGAC
ATCCTGCTGATAGACCTGCAAGTCGGAACAGCCAGCGGTTACGAAGTCGCCGAAGCCGCCCGCCGCTTCGGCCCCAACCG
GAATACGCCCATCATCGGCATGACGGCGTTCCGGCAGGAGTTCAACGATGCCCGCAGCGCGCTGCTGGCGGGCAAGCTGG
AAAAGCCGTTCAGCTTCGCCCAGCTGGAACAACTGCTGTCGCTGCACCTGCCGCGGAACAATACCGGCACGCCGGCGCAA
GCGTAA

Upstream 100 bases:

>100_bases
TCAGCCGCATCGAGGTCAAATGACTCGCTACTTCCGCCTCGTGTTCCGCAGCGTGGGGGGAATCGAACGCGCGTTCCTGG
CCCTGTCGCTGCTCGCGACC

Downstream 100 bases:

>100_bases
GCGCCGCGGCGAGCGGAGGCTGTTACCGGAACGCCACACACCATCTTGCCCGCTCCGGAATCGATGGGCACAACACCTTG
CCGGAAAAAACAAAAAAACC

Product: sensor/response regulator hybrid

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MIISFTLYAAVIKRPITNKVSDLYWMAAQMQLTFNRLEMDVWRYRAGQITREETDKSYAIAYSKYRMFTRPSAVNTDLEK
IEGFDQIKALLARIFQHPPRQWTQQDALELSRDMEEMRPLLADYGVKARYAENSLVAQQLRMVERHQTMYLLGLMCWLAF
LGIFWFVLHRLAEIRRHSLQQQQVLEREQAARAALVQTELARDTFLATISHEIRSPLQSIQTCTELLEYSIPPDNPGYGY
LSRLKQSNAYLLAQVRDIMDISALKNHQLALEPATTDIEELVACVVAVHQGNAEAKGLALTVAVPPMPLLWLDGNRLRQI
MWNLLSNAIRYTDQGGIRLELEYRPRNLVLSVADTGVGIPEDIRVQLFRPFTRGKTRRPGSSGLGLAIVHELVTLFGGQI
EIDSQEGRGSTFTLSLPVQEASTPEDCDGASYGQGRILLLDDDDPIRESYSALLQENGYSVSSFATVNDAAAQVVRHRFD
ILLIDLQVGTASGYEVAEAARRFGPNRNTPIIGMTAFRQEFNDARSALLAGKLEKPFSFAQLEQLLSLHLPRNNTGTPAQ
A

Sequences:

>Translated_561_residues
MIISFTLYAAVIKRPITNKVSDLYWMAAQMQLTFNRLEMDVWRYRAGQITREETDKSYAIAYSKYRMFTRPSAVNTDLEK
IEGFDQIKALLARIFQHPPRQWTQQDALELSRDMEEMRPLLADYGVKARYAENSLVAQQLRMVERHQTMYLLGLMCWLAF
LGIFWFVLHRLAEIRRHSLQQQQVLEREQAARAALVQTELARDTFLATISHEIRSPLQSIQTCTELLEYSIPPDNPGYGY
LSRLKQSNAYLLAQVRDIMDISALKNHQLALEPATTDIEELVACVVAVHQGNAEAKGLALTVAVPPMPLLWLDGNRLRQI
MWNLLSNAIRYTDQGGIRLELEYRPRNLVLSVADTGVGIPEDIRVQLFRPFTRGKTRRPGSSGLGLAIVHELVTLFGGQI
EIDSQEGRGSTFTLSLPVQEASTPEDCDGASYGQGRILLLDDDDPIRESYSALLQENGYSVSSFATVNDAAAQVVRHRFD
ILLIDLQVGTASGYEVAEAARRFGPNRNTPIIGMTAFRQEFNDARSALLAGKLEKPFSFAQLEQLLSLHLPRNNTGTPAQ
A
>Mature_561_residues
MIISFTLYAAVIKRPITNKVSDLYWMAAQMQLTFNRLEMDVWRYRAGQITREETDKSYAIAYSKYRMFTRPSAVNTDLEK
IEGFDQIKALLARIFQHPPRQWTQQDALELSRDMEEMRPLLADYGVKARYAENSLVAQQLRMVERHQTMYLLGLMCWLAF
LGIFWFVLHRLAEIRRHSLQQQQVLEREQAARAALVQTELARDTFLATISHEIRSPLQSIQTCTELLEYSIPPDNPGYGY
LSRLKQSNAYLLAQVRDIMDISALKNHQLALEPATTDIEELVACVVAVHQGNAEAKGLALTVAVPPMPLLWLDGNRLRQI
MWNLLSNAIRYTDQGGIRLELEYRPRNLVLSVADTGVGIPEDIRVQLFRPFTRGKTRRPGSSGLGLAIVHELVTLFGGQI
EIDSQEGRGSTFTLSLPVQEASTPEDCDGASYGQGRILLLDDDDPIRESYSALLQENGYSVSSFATVNDAAAQVVRHRFD
ILLIDLQVGTASGYEVAEAARRFGPNRNTPIIGMTAFRQEFNDARSALLAGKLEKPFSFAQLEQLLSLHLPRNNTGTPAQ
A

Specific function: At low cell density, in absence of AI-2 (autoinducer 2), luxQ has a kinase activity and autophosphorylates on a histidine residue. The phosphoryl group is then transferred to an aspartate residue in the response regulator domain. The phosphoryl group is t

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=392, Percent_Identity=28.3163265306122, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI145693157, Length=299, Percent_Identity=31.7725752508361, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788713, Length=380, Percent_Identity=28.1578947368421, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081816, Length=401, Percent_Identity=29.426433915212, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=33.7254901960784, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1790436, Length=272, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788393, Length=231, Percent_Identity=29.004329004329, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786912, Length=255, Percent_Identity=27.0588235294118, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI87082128, Length=225, Percent_Identity=29.7777777777778, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=29.4871794871795, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790861, Length=310, Percent_Identity=26.7741935483871, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=226, Percent_Identity=28.7610619469027, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787894, Length=221, Percent_Identity=26.6968325791855, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788549, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786783, Length=229, Percent_Identity=29.2576419213974, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR015387
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789
- ProDom:   PD142495 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF09308 LuxQ-periplasm; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63261; Mature: 63261

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISFTLYAAVIKRPITNKVSDLYWMAAQMQLTFNRLEMDVWRYRAGQITREETDKSYAI
CEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEE
AYSKYRMFTRPSAVNTDLEKIEGFDQIKALLARIFQHPPRQWTQQDALELSRDMEEMRPL
EEHHHHEEECCCCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LADYGVKARYAENSLVAQQLRMVERHQTMYLLGLMCWLAFLGIFWFVLHRLAEIRRHSLQ
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQQVLEREQAARAALVQTELARDTFLATISHEIRSPLQSIQTCTELLEYSIPPDNPGYGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
LSRLKQSNAYLLAQVRDIMDISALKNHQLALEPATTDIEELVACVVAVHQGNAEAKGLAL
HHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEE
TVAVPPMPLLWLDGNRLRQIMWNLLSNAIRYTDQGGIRLELEYRPRNLVLSVADTGVGIP
EEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCCCCC
EDIRVQLFRPFTRGKTRRPGSSGLGLAIVHELVTLFGGQIEIDSQEGRGSTFTLSLPVQE
HHHHHHEECCHHCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCC
ASTPEDCDGASYGQGRILLLDDDDPIRESYSALLQENGYSVSSFATVNDAAAQVVRHRFD
CCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEHEEHHHHHHHHHHHHHCC
ILLIDLQVGTASGYEVAEAARRFGPNRNTPIIGMTAFRQEFNDARSALLAGKLEKPFSFA
EEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHH
QLEQLLSLHLPRNNTGTPAQA
HHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIISFTLYAAVIKRPITNKVSDLYWMAAQMQLTFNRLEMDVWRYRAGQITREETDKSYAI
CEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEE
AYSKYRMFTRPSAVNTDLEKIEGFDQIKALLARIFQHPPRQWTQQDALELSRDMEEMRPL
EEHHHHEEECCCCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LADYGVKARYAENSLVAQQLRMVERHQTMYLLGLMCWLAFLGIFWFVLHRLAEIRRHSLQ
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQQVLEREQAARAALVQTELARDTFLATISHEIRSPLQSIQTCTELLEYSIPPDNPGYGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
LSRLKQSNAYLLAQVRDIMDISALKNHQLALEPATTDIEELVACVVAVHQGNAEAKGLAL
HHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEE
TVAVPPMPLLWLDGNRLRQIMWNLLSNAIRYTDQGGIRLELEYRPRNLVLSVADTGVGIP
EEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCCCCC
EDIRVQLFRPFTRGKTRRPGSSGLGLAIVHELVTLFGGQIEIDSQEGRGSTFTLSLPVQE
HHHHHHEECCHHCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCC
ASTPEDCDGASYGQGRILLLDDDDPIRESYSALLQENGYSVSSFATVNDAAAQVVRHRFD
CCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEHEEHHHHHHHHHHHHHCC
ILLIDLQVGTASGYEVAEAARRFGPNRNTPIIGMTAFRQEFNDARSALLAGKLEKPFSFA
EEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHH
QLEQLLSLHLPRNNTGTPAQA
HHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301; 12176318 [H]