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The map label for this gene is asnB [H]

Identifier: 34497168

GI number: 34497168

Start: 1852195

End: 1854045

Strand: Direct

Name: asnB [H]

Synonym: CV_1713

Alternate gene names: 34497168

Gene position: 1852195-1854045 (Clockwise)

Preceding gene: 34497167

Following gene: 34497170

Centisome position: 38.98

GC content: 69.48

Gene sequence:

>1851_bases
ATGTGTGGAATTGCCGGATGGGTGAGTTTCGAGCGTGATCTGAGCACGCAGCGCGCGGTCGCGGAGACGATGACGAAAAC
GATGGCCCTGCGCGGGCCGGACGACGAGGGACTGTGGCTGGACCGCCGCGCGGCGCTGGGGCATCGCCGCCTGTCCATCA
TCGACCTGGAGGGCGGCCGGCAGCCGATGACAGCCGACGAGGATGGCAGGACGCTGGCCTGCCTGGTGTACACCGGCGAG
GTGTACAACTTCCGGGAGCTGCGCCTTGAGCTGCAGGGCCTGGGCCACGCTTTTCGCACCCGCAGCGACACCGAGGTGGT
GCTGCGCGCCTATCTGCAGTGGGGCGAAGCGATGCTGGAGCGGCTGAACGGCATGTACGCGTTCGCGTTGTGGGACGCCC
GAGAACAGCGGCTGTTGCTGGTCCGCGACCGGATGGGCGTCAAGCCGCTGTACTACTATCCGATGCCGGACGGCGTGCTG
TTCGGCTCCGAGCCCAAGGCCATCCTGGCGCACCCCGAGGCCGAGCGACGGGTGACGGCCGGCGGCCTGCGCGAAATGCT
GGATCTGGTGAAGACGCCGGAGGCTGCGGCGTTCGCCGGCATGCACGAGGTGCGGCCCGGTCAGGTGGTGACGATAGACC
GTGGAGGCCTGCGCAAGCGCCGCTACTGGCAGCTGGAGGCGCGCGAGCACACCGACGACCTGCCGACCACCATCCGCACC
GTGCGCGAGCTGCTGGACGACATCGTCGAACGGCAGATCATCGCCGATGTGCCGCTATGCACGCTGTTGTCCGGCGGGCT
GGATTCGTCGGTGGTCACCGCGCTGGCCGCGCGGAGCATGCGGCGCCAGGGCAAGGGAACGGTGCGTTCGTTCTCGGTGG
ACTTCGTCAAACACGGCGCCGCGTTCGAGGCCGACTACCTGCGCGGCTCGCCGGACGCGCCCTTCGTGCGCGATCTGGCG
GCGCACGCCGGCACCGAGCATAGGGAAATTCTGCTGGAGAGCCGCGAGCTGGCCGATCCGCGCTTGCGCGCCGCGGTGCT
GGGCGCGCAGGATCTGCCGCCGGCGTATTGGGGCGACATGCTGCCGTCGCTGTACAAGCTGTTCGAGGCGATCCGGGCGC
AATCGACGGTGGCGCTGTCCGGCGAGTCGGCGGACGAGGTGTTCGGCGGCTATCGCTGGTTCCACGACGCCGCCGCGGTG
GACGGCGACGCGTTTCCGTGGCTGGCCGGGTCGAGCGGCGCCTTCTTCGACGGCCAGTCGCTGATCGCGCCGGGCCTGCT
GCGCGAGCTGGACATGCCGGGCTTCCGCCGCGACAGCTACGCGCAGGCGCTGGCCGAGGTGCCGCGGCTGGCGTCGGACA
GCACCCGCGAGCGGCGGATGCGCGAGATCAGCCATATGCACCTGACCCGCTTCGTCCAGGCGCTGCTGGACCGCAAGGAC
CGGATGAGCATGGCGGTGGGGCTGGAGGTGAGGGTGCCGTTCTGCGACCACCGGCTGGTGGAGTACGTGTTCAACGCGCC
GTGGAGCATGAAGAATTTCGACGGCCGGGAGAAGAGCCTGTTGCGCGCTGCCGGCAAGGACGTGCTGCCGGAGTCCATCG
TCCGGCGCGTCAAGAGCCCGTACCCGGCCACCCAGGATCCGGCTTACGAGCAGGCGCTGCGCCAGGGGCTGGCCCGGGTG
CTGGACGACTCCCGCTCGCCGGCCTTGCCTTTGCTGGACAAGGCCGCCGCGTGGAAGCTGCTGGACCGGCCATTCGGCAA
GACCAGCACCTCGCCGGAGCGCGCCGGGCTGGAAATGGCGCTGGGCCTGGATGAATGGCTGCGCCATCATCAGGTGTCGC
TGGACTTGTAA

Upstream 100 bases:

>100_bases
CCCGCTTTCGCGGCGGCCCCCTCTGCACTAATTATGCTTTAGGCATAATGCGAGCCGGCGGACCCCTTCGCCCGGCCGCT
GACAAGAGGAGAGCCGAACA

Downstream 100 bases:

>100_bases
ACCCGCTCAGCGGGGCGGCCGCACCGGCCGTCCCTGAATGGGCGTGGACAGGATGATGGCGGTGCGCGTCTCGCCCAGAT
CGTTCAGCTGCGCCACCAGC

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MCGIAGWVSFERDLSTQRAVAETMTKTMALRGPDDEGLWLDRRAALGHRRLSIIDLEGGRQPMTADEDGRTLACLVYTGE
VYNFRELRLELQGLGHAFRTRSDTEVVLRAYLQWGEAMLERLNGMYAFALWDAREQRLLLVRDRMGVKPLYYYPMPDGVL
FGSEPKAILAHPEAERRVTAGGLREMLDLVKTPEAAAFAGMHEVRPGQVVTIDRGGLRKRRYWQLEAREHTDDLPTTIRT
VRELLDDIVERQIIADVPLCTLLSGGLDSSVVTALAARSMRRQGKGTVRSFSVDFVKHGAAFEADYLRGSPDAPFVRDLA
AHAGTEHREILLESRELADPRLRAAVLGAQDLPPAYWGDMLPSLYKLFEAIRAQSTVALSGESADEVFGGYRWFHDAAAV
DGDAFPWLAGSSGAFFDGQSLIAPGLLRELDMPGFRRDSYAQALAEVPRLASDSTRERRMREISHMHLTRFVQALLDRKD
RMSMAVGLEVRVPFCDHRLVEYVFNAPWSMKNFDGREKSLLRAAGKDVLPESIVRRVKSPYPATQDPAYEQALRQGLARV
LDDSRSPALPLLDKAAAWKLLDRPFGKTSTSPERAGLEMALGLDEWLRHHQVSLDL

Sequences:

>Translated_616_residues
MCGIAGWVSFERDLSTQRAVAETMTKTMALRGPDDEGLWLDRRAALGHRRLSIIDLEGGRQPMTADEDGRTLACLVYTGE
VYNFRELRLELQGLGHAFRTRSDTEVVLRAYLQWGEAMLERLNGMYAFALWDAREQRLLLVRDRMGVKPLYYYPMPDGVL
FGSEPKAILAHPEAERRVTAGGLREMLDLVKTPEAAAFAGMHEVRPGQVVTIDRGGLRKRRYWQLEAREHTDDLPTTIRT
VRELLDDIVERQIIADVPLCTLLSGGLDSSVVTALAARSMRRQGKGTVRSFSVDFVKHGAAFEADYLRGSPDAPFVRDLA
AHAGTEHREILLESRELADPRLRAAVLGAQDLPPAYWGDMLPSLYKLFEAIRAQSTVALSGESADEVFGGYRWFHDAAAV
DGDAFPWLAGSSGAFFDGQSLIAPGLLRELDMPGFRRDSYAQALAEVPRLASDSTRERRMREISHMHLTRFVQALLDRKD
RMSMAVGLEVRVPFCDHRLVEYVFNAPWSMKNFDGREKSLLRAAGKDVLPESIVRRVKSPYPATQDPAYEQALRQGLARV
LDDSRSPALPLLDKAAAWKLLDRPFGKTSTSPERAGLEMALGLDEWLRHHQVSLDL
>Mature_616_residues
MCGIAGWVSFERDLSTQRAVAETMTKTMALRGPDDEGLWLDRRAALGHRRLSIIDLEGGRQPMTADEDGRTLACLVYTGE
VYNFRELRLELQGLGHAFRTRSDTEVVLRAYLQWGEAMLERLNGMYAFALWDAREQRLLLVRDRMGVKPLYYYPMPDGVL
FGSEPKAILAHPEAERRVTAGGLREMLDLVKTPEAAAFAGMHEVRPGQVVTIDRGGLRKRRYWQLEAREHTDDLPTTIRT
VRELLDDIVERQIIADVPLCTLLSGGLDSSVVTALAARSMRRQGKGTVRSFSVDFVKHGAAFEADYLRGSPDAPFVRDLA
AHAGTEHREILLESRELADPRLRAAVLGAQDLPPAYWGDMLPSLYKLFEAIRAQSTVALSGESADEVFGGYRWFHDAAAV
DGDAFPWLAGSSGAFFDGQSLIAPGLLRELDMPGFRRDSYAQALAEVPRLASDSTRERRMREISHMHLTRFVQALLDRKD
RMSMAVGLEVRVPFCDHRLVEYVFNAPWSMKNFDGREKSLLRAAGKDVLPESIVRRVKSPYPATQDPAYEQALRQGLARV
LDDSRSPALPLLDKAAAWKLLDRPFGKTSTSPERAGLEMALGLDEWLRHHQVSLDL

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=425, Percent_Identity=28, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI168229252, Length=425, Percent_Identity=28, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI168229250, Length=425, Percent_Identity=28, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI296010848, Length=396, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI296010852, Length=329, Percent_Identity=27.0516717325228, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI296010850, Length=329, Percent_Identity=27.0516717325228, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786889, Length=398, Percent_Identity=30.9045226130653, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71993933, Length=537, Percent_Identity=27.3743016759777, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25147557, Length=537, Percent_Identity=27.3743016759777, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325403, Length=599, Percent_Identity=26.5442404006678, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321563, Length=600, Percent_Identity=26.3333333333333, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45553209, Length=572, Percent_Identity=27.0979020979021, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69043; Mature: 69043

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGWVSFERDLSTQRAVAETMTKTMALRGPDDEGLWLDRRAALGHRRLSIIDLEGGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCEEEEEEECCCC
QPMTADEDGRTLACLVYTGEVYNFRELRLELQGLGHAFRTRSDTEVVLRAYLQWGEAMLE
CCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHH
RLNGMYAFALWDAREQRLLLVRDRMGVKPLYYYPMPDGVLFGSEPKAILAHPEAERRVTA
HHCCEEEEEEECCCCCCEEEEHHCCCCCEEEEECCCCCEEECCCCCEEEECCCHHHHHHH
GGLREMLDLVKTPEAAAFAGMHEVRPGQVVTIDRGGLRKRRYWQLEAREHTDDLPTTIRT
HHHHHHHHHHCCCCHHHHHCHHHCCCCCEEEECCCCCCCCCEEEEECHHCCCCCHHHHHH
VRELLDDIVERQIIADVPLCTLLSGGLDSSVVTALAARSMRRQGKGTVRSFSVDFVKHGA
HHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEHHHHHCCC
AFEADYLRGSPDAPFVRDLAAHAGTEHREILLESRELADPRLRAAVLGAQDLPPAYWGDM
CCCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
LPSLYKLFEAIRAQSTVALSGESADEVFGGYRWFHDAAAVDGDAFPWLAGSSGAFFDGQS
HHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCH
LIAPGLLRELDMPGFRRDSYAQALAEVPRLASDSTRERRMREISHMHLTRFVQALLDRKD
HHCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
RMSMAVGLEVRVPFCDHRLVEYVFNAPWSMKNFDGREKSLLRAAGKDVLPESIVRRVKSP
HHHHEECEEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCC
YPATQDPAYEQALRQGLARVLDDSRSPALPLLDKAAAWKLLDRPFGKTSTSPERAGLEMA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHC
LGLDEWLRHHQVSLDL
CCHHHHHHHHCCCCCC
>Mature Secondary Structure
MCGIAGWVSFERDLSTQRAVAETMTKTMALRGPDDEGLWLDRRAALGHRRLSIIDLEGGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCEEEEEEECCCC
QPMTADEDGRTLACLVYTGEVYNFRELRLELQGLGHAFRTRSDTEVVLRAYLQWGEAMLE
CCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHH
RLNGMYAFALWDAREQRLLLVRDRMGVKPLYYYPMPDGVLFGSEPKAILAHPEAERRVTA
HHCCEEEEEEECCCCCCEEEEHHCCCCCEEEEECCCCCEEECCCCCEEEECCCHHHHHHH
GGLREMLDLVKTPEAAAFAGMHEVRPGQVVTIDRGGLRKRRYWQLEAREHTDDLPTTIRT
HHHHHHHHHHCCCCHHHHHCHHHCCCCCEEEECCCCCCCCCEEEEECHHCCCCCHHHHHH
VRELLDDIVERQIIADVPLCTLLSGGLDSSVVTALAARSMRRQGKGTVRSFSVDFVKHGA
HHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHEEHHHHHCCC
AFEADYLRGSPDAPFVRDLAAHAGTEHREILLESRELADPRLRAAVLGAQDLPPAYWGDM
CCCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
LPSLYKLFEAIRAQSTVALSGESADEVFGGYRWFHDAAAVDGDAFPWLAGSSGAFFDGQS
HHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCH
LIAPGLLRELDMPGFRRDSYAQALAEVPRLASDSTRERRMREISHMHLTRFVQALLDRKD
HHCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
RMSMAVGLEVRVPFCDHRLVEYVFNAPWSMKNFDGREKSLLRAAGKDVLPESIVRRVKSP
HHHHEECEEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCC
YPATQDPAYEQALRQGLARVLDDSRSPALPLLDKAAAWKLLDRPFGKTSTSPERAGLEMA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHC
LGLDEWLRHHQVSLDL
CCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]