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The map label for this gene is cls [C]

Identifier: 34497167

GI number: 34497167

Start: 1850816

End: 1852030

Strand: Direct

Name: cls [C]

Synonym: CV_1712

Alternate gene names: 34497167

Gene position: 1850816-1852030 (Clockwise)

Preceding gene: 34497166

Following gene: 34497168

Centisome position: 38.96

GC content: 67.24

Gene sequence:

>1215_bases
ATGTTCAAATACGGGTGGCCGCTGGCCCTGATCCTGGCCGCGCCGCTGGCCCGCGCCGACTTCTCCATCCCCGGTTTCGA
GCTGGTGCACACCGCGCCGCAGCGCGCCGGCCTGAACACGCCGGATCTGCGCGATCCGGCCGCGGTGTGGCGGCAGATGT
TCGATTCGGCCAAGCGCGAAATCGCGCTGGAGCAGTTCTATGTAGCCGGGCAGCCGGGCAGCCTGCTGGACGGCGTGATC
GAGCGGCTGGACGCCGCCGGGCGCCGCGGCGTGAAGATCCGCTTCCTGATGGAGAAGAAGGGCATGTTCGCCAGCGACCC
GGCGACGATAGAACGGCTCCAGCGCATCCCCAATCTGGAATACCGCCAGCTGGACTACAACCAGCTGACCGGCAACGGCA
TCATCCACGCCAAGTTCATCGTGGCGGACGGCAAGTCCGCCTATGTCGGCAGCCAGAATTTCGACTGGCGCTCGCTGCAG
CACATCCACGAAACCGGGCTCAAGGTCGACGACGCGGCGATGGCCGGCCGCATGCAGGCCATCTTCGAACAGGACTGGGC
GGCGCAGGCGACGCTGGCCGCCGGCGGAAAGGTCAAGCCGCTGCGGACGGCCGAGCAGGCGGCCGACCTGTCGCAGCCGG
CGGCGCTGCTGGCCAGCCCCAACGCCTACAATCCGGACGGCGTCGGCGATTCCGAAGAAGCGCTGCCGCGGCTGATGGCC
GAGGCGAAGCGCGAGGTGCGCATCCAGCTGCTGGATTACGCGCCGCTCAGCTTCGGCAAGCCGCGCAGCTACTATCCGGT
TTTCGACAACGCCATCCGCGGCGCGTTGGCGCGCGGCGTCAAGGTCAAGCTGATGGTGTCGGACTGGAATCTGGAAAAGC
CGGGCGTGGACTATCTGAAAAGCCTGGCGGTGCTGCCCGGCATGGAGCTGCGCATCGTCACCATTCCGCGCGACGGCACC
TGCATCCCCTTCGCCCGCGTCATCCACAGCAAGACCATGGAGATAGACGGCAGCATAGCCTGGGTGGGCACCAGCAACTG
GAGCGGCGGCTATATGGACAAGTCGCGCAATGTCGAGGTGGTATTGCGCAACGAGGCGATGGCCAGGCGCATCGCCGAAC
TGCACGAGCAGACCTGGTCCTCGCCGCATGCGGTGCCGCTGGAGGTGTCCCGCCAGTATCCGGCGCCGGACAAGGCCTGC
CGCCAGGCCGGCTGA

Upstream 100 bases:

>100_bases
GTCTTGACGGCTGTCCCTCCCGGGCAGCCTTTTTTCATGCCGGCTACATACCTGCTTAACATGTCCATGGCATGATTAAC
AGTTTCGCAAGGAAGCCGCA

Downstream 100 bases:

>100_bases
GGTCGAAGCTCGGCGGGGGCCGGGCTTCGACAAAATACGTCAATATCCCATTCTTATTACCAGTCCCGCTTTCGCGGCGG
CCCCCTCTGCACTAATTATG

Product: phospholipase D/ transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: Phospholipase D/Transphosphatidylase; Phospholipase D/ Transphosphatidylase; Phospholipase D

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MFKYGWPLALILAAPLARADFSIPGFELVHTAPQRAGLNTPDLRDPAAVWRQMFDSAKREIALEQFYVAGQPGSLLDGVI
ERLDAAGRRGVKIRFLMEKKGMFASDPATIERLQRIPNLEYRQLDYNQLTGNGIIHAKFIVADGKSAYVGSQNFDWRSLQ
HIHETGLKVDDAAMAGRMQAIFEQDWAAQATLAAGGKVKPLRTAEQAADLSQPAALLASPNAYNPDGVGDSEEALPRLMA
EAKREVRIQLLDYAPLSFGKPRSYYPVFDNAIRGALARGVKVKLMVSDWNLEKPGVDYLKSLAVLPGMELRIVTIPRDGT
CIPFARVIHSKTMEIDGSIAWVGTSNWSGGYMDKSRNVEVVLRNEAMARRIAELHEQTWSSPHAVPLEVSRQYPAPDKAC
RQAG

Sequences:

>Translated_404_residues
MFKYGWPLALILAAPLARADFSIPGFELVHTAPQRAGLNTPDLRDPAAVWRQMFDSAKREIALEQFYVAGQPGSLLDGVI
ERLDAAGRRGVKIRFLMEKKGMFASDPATIERLQRIPNLEYRQLDYNQLTGNGIIHAKFIVADGKSAYVGSQNFDWRSLQ
HIHETGLKVDDAAMAGRMQAIFEQDWAAQATLAAGGKVKPLRTAEQAADLSQPAALLASPNAYNPDGVGDSEEALPRLMA
EAKREVRIQLLDYAPLSFGKPRSYYPVFDNAIRGALARGVKVKLMVSDWNLEKPGVDYLKSLAVLPGMELRIVTIPRDGT
CIPFARVIHSKTMEIDGSIAWVGTSNWSGGYMDKSRNVEVVLRNEAMARRIAELHEQTWSSPHAVPLEVSRQYPAPDKAC
RQAG
>Mature_404_residues
MFKYGWPLALILAAPLARADFSIPGFELVHTAPQRAGLNTPDLRDPAAVWRQMFDSAKREIALEQFYVAGQPGSLLDGVI
ERLDAAGRRGVKIRFLMEKKGMFASDPATIERLQRIPNLEYRQLDYNQLTGNGIIHAKFIVADGKSAYVGSQNFDWRSLQ
HIHETGLKVDDAAMAGRMQAIFEQDWAAQATLAAGGKVKPLRTAEQAADLSQPAALLASPNAYNPDGVGDSEEALPRLMA
EAKREVRIQLLDYAPLSFGKPRSYYPVFDNAIRGALARGVKVKLMVSDWNLEKPGVDYLKSLAVLPGMELRIVTIPRDGT
CIPFARVIHSKTMEIDGSIAWVGTSNWSGGYMDKSRNVEVVLRNEAMARRIAELHEQTWSSPHAVPLEVSRQYPAPDKAC
RQAG

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI94681038, Length=416, Percent_Identity=27.1634615384615, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI166197683, Length=354, Percent_Identity=27.683615819209, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI72534684, Length=354, Percent_Identity=27.683615819209, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI22749353, Length=351, Percent_Identity=22.5071225071225, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559458, Length=392, Percent_Identity=29.3367346938776, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI193207667, Length=396, Percent_Identity=29.5454545454545, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32566758, Length=412, Percent_Identity=27.9126213592233, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17565994, Length=412, Percent_Identity=27.9126213592233, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI193208604, Length=424, Percent_Identity=25.7075471698113, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17532785, Length=365, Percent_Identity=25.7534246575342, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585591, Length=416, Percent_Identity=27.8846153846154, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20129681, Length=416, Percent_Identity=27.8846153846154, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI19921618, Length=289, Percent_Identity=24.9134948096886, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.8.- [C]

Molecular weight: Translated: 44639; Mature: 44639

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKYGWPLALILAAPLARADFSIPGFELVHTAPQRAGLNTPDLRDPAAVWRQMFDSAKRE
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHH
IALEQFYVAGQPGSLLDGVIERLDAAGRRGVKIRFLMEKKGMFASDPATIERLQRIPNLE
HHHHHHEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCC
YRQLDYNQLTGNGIIHAKFIVADGKSAYVGSQNFDWRSLQHIHETGLKVDDAAMAGRMQA
EEECCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
IFEQDWAAQATLAAGGKVKPLRTAEQAADLSQPAALLASPNAYNPDGVGDSEEALPRLMA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHH
EAKREVRIQLLDYAPLSFGKPRSYYPVFDNAIRGALARGVKVKLMVSDWNLEKPGVDYLK
HHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
SLAVLPGMELRIVTIPRDGTCIPFARVIHSKTMEIDGSIAWVGTSNWSGGYMDKSRNVEV
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCEEECCCEEEEECCCCCCCCCCCCCCEEE
VLRNEAMARRIAELHEQTWSSPHAVPLEVSRQYPAPDKACRQAG
EECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCC
>Mature Secondary Structure
MFKYGWPLALILAAPLARADFSIPGFELVHTAPQRAGLNTPDLRDPAAVWRQMFDSAKRE
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHH
IALEQFYVAGQPGSLLDGVIERLDAAGRRGVKIRFLMEKKGMFASDPATIERLQRIPNLE
HHHHHHEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCC
YRQLDYNQLTGNGIIHAKFIVADGKSAYVGSQNFDWRSLQHIHETGLKVDDAAMAGRMQA
EEECCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
IFEQDWAAQATLAAGGKVKPLRTAEQAADLSQPAALLASPNAYNPDGVGDSEEALPRLMA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHH
EAKREVRIQLLDYAPLSFGKPRSYYPVFDNAIRGALARGVKVKLMVSDWNLEKPGVDYLK
HHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
SLAVLPGMELRIVTIPRDGTCIPFARVIHSKTMEIDGSIAWVGTSNWSGGYMDKSRNVEV
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCEEECCCEEEEECCCCCCCCCCCCCCEEE
VLRNEAMARRIAELHEQTWSSPHAVPLEVSRQYPAPDKACRQAG
EECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA