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The map label for this gene is grpE

Identifier: 34497097

GI number: 34497097

Start: 1778604

End: 1779170

Strand: Direct

Name: grpE

Synonym: CV_1642

Alternate gene names: 34497097

Gene position: 1778604-1779170 (Clockwise)

Preceding gene: 34497094

Following gene: 34497098

Centisome position: 37.44

GC content: 64.37

Gene sequence:

>567_bases
GTGCAATTCCGAGGAAACGTCATGCAAGAGAACCAAAACCAAGCGGCCGAAGCCGTGGAAAACGAAATGACCGAGACCGC
CAGCGCGGCGCATACCGAGCTGGAGCAGACCCCCGAGCAGCGCATCGCCGGGCTGGAAGCCGAAATCGCCGAACTGAACG
ACACCCTGCTGCGCGCCCGCGCCGAGCTGGAAAACCAGCGCCGCCGCGCGCAGGACGAGGTGGCCGCCGCGCACAAATAC
GCGATCGGCAAGTTCGCGGCCGAGCTGGTGACGGTCAAGGATTATCTGGAAATGGCCCTGCTGGACCAGAGCGGCCAGAT
CGACGCGTTGAAGATGGGCGTGGACATGACGCTGAAACAGCTGGTGTCCGCCTTCGACAAGGCGCAGATCAAGGACATCG
CGCCCAAGCTGGGCGACAAGCTGGATCCCCACCAGCACCAGGCGATGAGCGCGGAAGAGTCCGACGCCGAGCCGAACACC
GTGGTGCGCGTGATGCAGAAGGGCTATTTGCTGGCCGACCGCGTGCTGCGCCCGGCCATGGTGGTGGTGGCCAAGGCCAA
GGCATAA

Upstream 100 bases:

>100_bases
ACGCACATTGACTATTTTTGCCTTGAGATGGAGATATGCGCGTCAGGGCTTGAAATCGGGACGAAAGTCCCTACCTTGCA
TCTGGACATCAATTTAGCCG

Downstream 100 bases:

>100_bases
AAAAGGCGCATGCCGGCCTCTTGAAAAGTTCGGCATAGCCAATATTTATTGAGACAACAGGCTCTGACTATCAGATAAGT
CAAAGCCGGAACGAATAGAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MQFRGNVMQENQNQAAEAVENEMTETASAAHTELEQTPEQRIAGLEAEIAELNDTLLRARAELENQRRRAQDEVAAAHKY
AIGKFAAELVTVKDYLEMALLDQSGQIDALKMGVDMTLKQLVSAFDKAQIKDIAPKLGDKLDPHQHQAMSAEESDAEPNT
VVRVMQKGYLLADRVLRPAMVVVAKAKA

Sequences:

>Translated_188_residues
MQFRGNVMQENQNQAAEAVENEMTETASAAHTELEQTPEQRIAGLEAEIAELNDTLLRARAELENQRRRAQDEVAAAHKY
AIGKFAAELVTVKDYLEMALLDQSGQIDALKMGVDMTLKQLVSAFDKAQIKDIAPKLGDKLDPHQHQAMSAEESDAEPNT
VVRVMQKGYLLADRVLRPAMVVVAKAKA
>Mature_188_residues
MQFRGNVMQENQNQAAEAVENEMTETASAAHTELEQTPEQRIAGLEAEIAELNDTLLRARAELENQRRRAQDEVAAAHKY
AIGKFAAELVTVKDYLEMALLDQSGQIDALKMGVDMTLKQLVSAFDKAQIKDIAPKLGDKLDPHQHQAMSAEESDAEPNT
VVRVMQKGYLLADRVLRPAMVVVAKAKA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=150, Percent_Identity=35.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI40255109, Length=151, Percent_Identity=34.4370860927152, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1788967, Length=186, Percent_Identity=40.3225806451613, Blast_Score=105, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324806, Length=156, Percent_Identity=33.974358974359, Blast_Score=82, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24653432, Length=187, Percent_Identity=31.5508021390374, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CHRVO (Q7NXI4)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_901312.1
- ProteinModelPortal:   Q7NXI4
- SMR:   Q7NXI4
- GeneID:   2550367
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1642
- NMPDR:   fig|243365.1.peg.1642
- HOGENOM:   HBG732630
- OMA:   HEAIAYV
- PhylomeDB:   Q7NXI4
- ProtClustDB:   PRK14140
- BioCyc:   CVIO243365:CV_1642-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20770; Mature: 20770

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFRGNVMQENQNQAAEAVENEMTETASAAHTELEQTPEQRIAGLEAEIAELNDTLLRAR
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHHHHH
AELENQRRRAQDEVAAAHKYAIGKFAAELVTVKDYLEMALLDQSGQIDALKMGVDMTLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LVSAFDKAQIKDIAPKLGDKLDPHQHQAMSAEESDAEPNTVVRVMQKGYLLADRVLRPAM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHH
VVVAKAKA
HHHCCCCC
>Mature Secondary Structure
MQFRGNVMQENQNQAAEAVENEMTETASAAHTELEQTPEQRIAGLEAEIAELNDTLLRAR
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHHHHH
AELENQRRRAQDEVAAAHKYAIGKFAAELVTVKDYLEMALLDQSGQIDALKMGVDMTLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LVSAFDKAQIKDIAPKLGDKLDPHQHQAMSAEESDAEPNTVVRVMQKGYLLADRVLRPAM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHH
VVVAKAKA
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782