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The map label for this gene is fabI [H]

Identifier: 34497038

GI number: 34497038

Start: 1707187

End: 1707975

Strand: Reverse

Name: fabI [H]

Synonym: CV_1583

Alternate gene names: 34497038

Gene position: 1707975-1707187 (Counterclockwise)

Preceding gene: 34497042

Following gene: 34497033

Centisome position: 35.95

GC content: 65.02

Gene sequence:

>789_bases
ATGGGCTTTCTGCAAGGCAAGAAAATCCTGATCACCGGCATGATCTCCAATCGCTCCATCGCCTACGGCATCGCTCAGGC
CTGTCATCGCGAAGGCGCCGAGCTGGCCTTCACTTACGTGGTGGACAAGCTGGAGGAACGCGTCCGTGAAATGGCGGCCG
ACTTCGGCTCCCAGCTGGTCTACCGCTGCGACGTGCAGAACGACGACGAGATCAACCAGCTGTTCGTCGACCTCGCCAAG
GAATGGGACGGTCTGGACGGCCTGGTGCACTCGATCGGCTTCGCCCCGCGCGAAGCGCTGGAAGGCGACTTCCTGGATTC
TCTGAGCCGCGAAGCGTTCCAGATCGCCCACGACGTGTCGTCCTACAGCTTCCCGGCGCTGGCCAAGGCCGCGCGCCCGA
TGATGCAGGGCCGCAAGGCCGCGCTGCTGACCCTGTCCTACCTGGGCGCGGTCCGCGCGATCCCGAACTACAACGTGATG
GGCCTGGCCAAGGCCAGCCTGGAGGCGTCGGTGCGCTTCATGGCCGCCAGCCTGGGCAAGGAAGGCATCCGCGTCAACGG
CATCTCCGCCGGTCCGATCAAGACCCTGGCCGCCTCCGGCATCTCCGGCTTCTCCAAGCTGCTGAACATGGCGTCCAGCC
AGGCTTGCCTGCGCCGCAACGTGACCACCGAGGAAGTCGGCAACGCCGCCGCCTTCATGCTGTCGGACCTGTCGTCCGGC
ATCACCGGCGAGATCACCTACGTCGACGCCGGCTACAGCGTCAACGCGCTGAACGTGCCGGAAGAATAA

Upstream 100 bases:

>100_bases
GGGTTATCCTTGCTGAAACGGGCAGTTAGCGCAGACGACGCTGCTGCAAATCGCCGGCATTCGGTATAATGACCGGCTGT
TTACATGAGAAGGACACCAA

Downstream 100 bases:

>100_bases
GCGCTTTCGCGCGACCCAAGGCAGACGGCGCGGTTTTCCCGCGCCGTTTTTCATTCACGCGCCGCAATCGGCGCCGGCCA
GCGTCCGCAAACCGCCGCCG

Product: enoyl-ACP reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MGFLQGKKILITGMISNRSIAYGIAQACHREGAELAFTYVVDKLEERVREMAADFGSQLVYRCDVQNDDEINQLFVDLAK
EWDGLDGLVHSIGFAPREALEGDFLDSLSREAFQIAHDVSSYSFPALAKAARPMMQGRKAALLTLSYLGAVRAIPNYNVM
GLAKASLEASVRFMAASLGKEGIRVNGISAGPIKTLAASGISGFSKLLNMASSQACLRRNVTTEEVGNAAAFMLSDLSSG
ITGEITYVDAGYSVNALNVPEE

Sequences:

>Translated_262_residues
MGFLQGKKILITGMISNRSIAYGIAQACHREGAELAFTYVVDKLEERVREMAADFGSQLVYRCDVQNDDEINQLFVDLAK
EWDGLDGLVHSIGFAPREALEGDFLDSLSREAFQIAHDVSSYSFPALAKAARPMMQGRKAALLTLSYLGAVRAIPNYNVM
GLAKASLEASVRFMAASLGKEGIRVNGISAGPIKTLAASGISGFSKLLNMASSQACLRRNVTTEEVGNAAAFMLSDLSSG
ITGEITYVDAGYSVNALNVPEE
>Mature_261_residues
GFLQGKKILITGMISNRSIAYGIAQACHREGAELAFTYVVDKLEERVREMAADFGSQLVYRCDVQNDDEINQLFVDLAKE
WDGLDGLVHSIGFAPREALEGDFLDSLSREAFQIAHDVSSYSFPALAKAARPMMQGRKAALLTLSYLGAVRAIPNYNVMG
LAKASLEASVRFMAASLGKEGIRVNGISAGPIKTLAASGISGFSKLLNMASSQACLRRNVTTEEVGNAAAFMLSDLSSGI
TGEITYVDAGYSVNALNVPEE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=62.4031007751938, Blast_Score=330, Evalue=7e-92,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=29.277566539924, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28102; Mature: 27971

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLQGKKILITGMISNRSIAYGIAQACHREGAELAFTYVVDKLEERVREMAADFGSQLV
CCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
YRCDVQNDDEINQLFVDLAKEWDGLDGLVHSIGFAPREALEGDFLDSLSREAFQIAHDVS
EEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHH
SYSFPALAKAARPMMQGRKAALLTLSYLGAVRAIPNYNVMGLAKASLEASVRFMAASLGK
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCC
EGIRVNGISAGPIKTLAASGISGFSKLLNMASSQACLRRNVTTEEVGNAAAFMLSDLSSG
CCCEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
ITGEITYVDAGYSVNALNVPEE
CCEEEEEEECCCEEEECCCCCC
>Mature Secondary Structure 
GFLQGKKILITGMISNRSIAYGIAQACHREGAELAFTYVVDKLEERVREMAADFGSQLV
CCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
YRCDVQNDDEINQLFVDLAKEWDGLDGLVHSIGFAPREALEGDFLDSLSREAFQIAHDVS
EEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHH
SYSFPALAKAARPMMQGRKAALLTLSYLGAVRAIPNYNVMGLAKASLEASVRFMAASLGK
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCC
EGIRVNGISAGPIKTLAASGISGFSKLLNMASSQACLRRNVTTEEVGNAAAFMLSDLSSG
CCCEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
ITGEITYVDAGYSVNALNVPEE
CCEEEEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]