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The map label for this gene is prpB [C]

Identifier: 34496973

GI number: 34496973

Start: 1625021

End: 1625929

Strand: Direct

Name: prpB [C]

Synonym: CV_1518

Alternate gene names: 34496973

Gene position: 1625021-1625929 (Clockwise)

Preceding gene: 34496966

Following gene: 34496974

Centisome position: 34.2

GC content: 71.51

Gene sequence:

>909_bases
TTGTACCATGCCGCCGGTATCTTGCCGGGAAGCCAAACCGCCGACCGACAAGGAATGACAATGGATCATCACGACGCCGT
ATCCCGCGCCCGGACCTTCCGCCGCCTGCATCAGGACGAGCGGCCGCTGCTGTTGCCGACTGTCTGGGACGCATTGAGCG
CCCGGGTGTTCGAGCAGGCCGGATTTCCCGCCTTGGCCACCAGCAGCGCCGCGGTGGCGTGGTCGCTTGGCTACCGCGAC
GGCGGCGAACTGCCGCGCGAGGCGCTGCTGGCCGCCGTCGCCCGCATCTCCAGGGTGCTGCAGGCGCCGCTGAGCGTCGA
CCTTGAAGACGGTTTCGGCGATACGCCGGCCGCGGTGGGGGAAACCGTCGCCGCGGTGATCCGCGCCGGCGCGGTCGGCG
TCAATCTGGAGGATGGCCGCTACGACGCCGAAGGGCGCTTCTCCTTGCGCGACGCGGACGAGATGCGCGCCCGCCTGCAA
GCCGCCCGCGCCGCCGCCGACGCGCTGGAGGTGCCGATCTTCATCAATGCCCGCAGCGACCTGTTCCTGCACGGCGAAGG
TCCGGCCGAGGCCCGCTTCGACGAGACGCTGGCGCGGGCCCGGCTATATCTGGACAGCGGCGCCGATGGCGTCTTTCCCA
TCGGCCTGACCGATCCCGCTTTGCTGGCGCGGCTGTGCGGGGCGGTGCCGGCGCCGGTCAATGCCGCCGCGATGCCGGGC
CTGCCCGGCTTGTCGGAGCTGGCGGCGCTCGGCGTGGCGCGCGTCAGCACCGCCATCGGCCCGGCGCTGGTGGCGATGCA
GGCATTGAAACAGACGGCAGCGGCGCTGCATGACGCGGGCGATTTGTCGGCGTTGAGAACGCCGCTGGGCTACGCCGACG
CCCAGGCGCTGTTCGCGCCGCGCGGATGA

Upstream 100 bases:

>100_bases
AGCTGCCGGTGCAGCGGCGGCGCGTGCTCGCGCAGCAAGGGGAACTGGAACGCGGAGGGAATCATGGCCTATTGGTTTTG
ACGATTTTTGGCTCTCATCA

Downstream 100 bases:

>100_bases
CACCCATCCCTACTCAGAATTTTTTTTACAGGAGCACTGATATGTCCCGTTTCATCGATTACCCGACGCTAGCGCCGCAA
GGCTACCAGGCCCAGGCTGG

Product: carboxyvinyl-carboxyphosphonate phosphorylmutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MYHAAGILPGSQTADRQGMTMDHHDAVSRARTFRRLHQDERPLLLPTVWDALSARVFEQAGFPALATSSAAVAWSLGYRD
GGELPREALLAAVARISRVLQAPLSVDLEDGFGDTPAAVGETVAAVIRAGAVGVNLEDGRYDAEGRFSLRDADEMRARLQ
AARAAADALEVPIFINARSDLFLHGEGPAEARFDETLARARLYLDSGADGVFPIGLTDPALLARLCGAVPAPVNAAAMPG
LPGLSELAALGVARVSTAIGPALVAMQALKQTAAALHDAGDLSALRTPLGYADAQALFAPRG

Sequences:

>Translated_302_residues
MYHAAGILPGSQTADRQGMTMDHHDAVSRARTFRRLHQDERPLLLPTVWDALSARVFEQAGFPALATSSAAVAWSLGYRD
GGELPREALLAAVARISRVLQAPLSVDLEDGFGDTPAAVGETVAAVIRAGAVGVNLEDGRYDAEGRFSLRDADEMRARLQ
AARAAADALEVPIFINARSDLFLHGEGPAEARFDETLARARLYLDSGADGVFPIGLTDPALLARLCGAVPAPVNAAAMPG
LPGLSELAALGVARVSTAIGPALVAMQALKQTAAALHDAGDLSALRTPLGYADAQALFAPRG
>Mature_302_residues
MYHAAGILPGSQTADRQGMTMDHHDAVSRARTFRRLHQDERPLLLPTVWDALSARVFEQAGFPALATSSAAVAWSLGYRD
GGELPREALLAAVARISRVLQAPLSVDLEDGFGDTPAAVGETVAAVIRAGAVGVNLEDGRYDAEGRFSLRDADEMRARLQ
AARAAADALEVPIFINARSDLFLHGEGPAEARFDETLARARLYLDSGADGVFPIGLTDPALLARLCGAVPAPVNAAAMPG
LPGLSELAALGVARVSTAIGPALVAMQALKQTAAALHDAGDLSALRTPLGYADAQALFAPRG

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=256, Percent_Identity=32.03125, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 31519; Mature: 31519

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYHAAGILPGSQTADRQGMTMDHHDAVSRARTFRRLHQDERPLLLPTVWDALSARVFEQA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHC
GFPALATSSAAVAWSLGYRDGGELPREALLAAVARISRVLQAPLSVDLEDGFGDTPAAVG
CCCCEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHH
ETVAAVIRAGAVGVNLEDGRYDAEGRFSLRDADEMRARLQAARAAADALEVPIFINARSD
HHHHHHHHHCCCEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCC
LFLHGEGPAEARFDETLARARLYLDSGADGVFPIGLTDPALLARLCGAVPAPVNAAAMPG
EEEECCCCCHHHHHHHHHHHHHHEECCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCC
LPGLSELAALGVARVSTAIGPALVAMQALKQTAAALHDAGDLSALRTPLGYADAQALFAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHCCC
RG
CC
>Mature Secondary Structure
MYHAAGILPGSQTADRQGMTMDHHDAVSRARTFRRLHQDERPLLLPTVWDALSARVFEQA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHC
GFPALATSSAAVAWSLGYRDGGELPREALLAAVARISRVLQAPLSVDLEDGFGDTPAAVG
CCCCEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHH
ETVAAVIRAGAVGVNLEDGRYDAEGRFSLRDADEMRARLQAARAAADALEVPIFINARSD
HHHHHHHHHCCCEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCC
LFLHGEGPAEARFDETLARARLYLDSGADGVFPIGLTDPALLARLCGAVPAPVNAAAMPG
EEEECCCCCHHHHHHHHHHHHHHEECCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCC
LPGLSELAALGVARVSTAIGPALVAMQALKQTAAALHDAGDLSALRTPLGYADAQALFAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHCCC
RG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]