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The map label for this gene is pilU1 [H]

Identifier: 34496913

GI number: 34496913

Start: 1545762

End: 1546892

Strand: Reverse

Name: pilU1 [H]

Synonym: CV_1458

Alternate gene names: 34496913

Gene position: 1546892-1545762 (Counterclockwise)

Preceding gene: 34496914

Following gene: 34496912

Centisome position: 32.56

GC content: 62.6

Gene sequence:

>1131_bases
ATGCTGATGTTGCCGTTTTTCAAACTGATGGCCGAAAAAAAGGCCAGCGACCTGTTTCTGACCAGCTTCTCCCCGCCGAT
GCTGAAGCTGGGCGGCGAACTGACGCCGGTCAACGAGAAGCCGCTGACGCCGGACATGGTCAAGCAACTGGCCTACAGCC
TGATGAGCGAGGAAGAAGCCGCCGTCTTCGAGCGCGAGCGCGAGATGAATCTGGGCCATCCGGTGCCGGGCCTGGGCAGC
TTCCGCGTCAACGTGTTCTGGCAGCGCGGCTCGGTCGGCATGGTGGTGCGCTACATCCTGCCCAGCATCCCGACCGTGGA
AGAGCTGCAGTTGCCGCCGCTGCTGGAAGAGCTGATCAAGCTGAAGCGCGGCCTGATCCTGGTGGTCGGCTCCACCGGCT
CCGGCAAAAGCTCGACGGTGGCGGCGATGCTGCAATCGCGCAACCGCAGCATGAAGGGCCACATCCTCACCATCGAGGAT
CCGATCGAATTCCTGTTCCGCCACGACCAGTCCATAGTCAACCAGCGCGAGATCGGCTTCGACACGCTGTCCTACGCCCG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGCTGATGATAGGCGAGATCCGCGATCCGGAAACGCTGAGCCAGG
CCATCGGCTACGCCCAATCCGGCCATCTGTGCATCTCCACGCTGCACGGCAACAACAGCTACCACATGCTGAACCGGGTG
ATGAATTTCTTTCCGGCAGAAGCGCGCGGCGCGCTGCTGATGGACCTGTCGGCCTCGCTGCGCGCCGTGATCTCGCAGCG
GCTGGTGGCCACGCTGGACGGCGGCCGGGTGCCTGCGCTGGAGCTGCTGATCAACACGCCGCACATTTCGGAGTTGATTC
GGGCCGGAGAGATGGACAAAATCAAGGAGGCCATCGAAAGCAGCATGAGCGATGGCGCGCAAACCTTCGAACAGGCGCTG
TTCCAGCTCTACCAGGCCGGACGGATCAGCCTGGACGAAGCGTTGAAGAACGCCGACTCGCCCACCAATCTGTACTGGCT
GATCAACCAGTCCCAGGAGGCGGGCAAAGGCAAAGACAAACCTGGCCCCGCCAGCGCGGCCAGCTTCGACAGCATCAAAC
TGGACTTGTAA

Upstream 100 bases:

>100_bases
ACGCCGCCCCCTTGGGCGGCGTTTTGTTTTACCCGGCGCAAACGCAAGCCCGCGCGGGACGGCTAAACTGAATCGATCCT
CAGCCCCTTCGGAGCGCTCC

Downstream 100 bases:

>100_bases
ACCCACCCGATGATTACCCTGTACGGCATTCCCAACTGCAATACCGTGAAGAAGGCCCGCCAATGGCTGGCCGACAACGG
CATCGACTACCTCTTCCACG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MLMLPFFKLMAEKKASDLFLTSFSPPMLKLGGELTPVNEKPLTPDMVKQLAYSLMSEEEAAVFEREREMNLGHPVPGLGS
FRVNVFWQRGSVGMVVRYILPSIPTVEELQLPPLLEELIKLKRGLILVVGSTGSGKSSTVAAMLQSRNRSMKGHILTIED
PIEFLFRHDQSIVNQREIGFDTLSYARALKNAMREAPDVLMIGEIRDPETLSQAIGYAQSGHLCISTLHGNNSYHMLNRV
MNFFPAEARGALLMDLSASLRAVISQRLVATLDGGRVPALELLINTPHISELIRAGEMDKIKEAIESSMSDGAQTFEQAL
FQLYQAGRISLDEALKNADSPTNLYWLINQSQEAGKGKDKPGPASAASFDSIKLDL

Sequences:

>Translated_376_residues
MLMLPFFKLMAEKKASDLFLTSFSPPMLKLGGELTPVNEKPLTPDMVKQLAYSLMSEEEAAVFEREREMNLGHPVPGLGS
FRVNVFWQRGSVGMVVRYILPSIPTVEELQLPPLLEELIKLKRGLILVVGSTGSGKSSTVAAMLQSRNRSMKGHILTIED
PIEFLFRHDQSIVNQREIGFDTLSYARALKNAMREAPDVLMIGEIRDPETLSQAIGYAQSGHLCISTLHGNNSYHMLNRV
MNFFPAEARGALLMDLSASLRAVISQRLVATLDGGRVPALELLINTPHISELIRAGEMDKIKEAIESSMSDGAQTFEQAL
FQLYQAGRISLDEALKNADSPTNLYWLINQSQEAGKGKDKPGPASAASFDSIKLDL
>Mature_376_residues
MLMLPFFKLMAEKKASDLFLTSFSPPMLKLGGELTPVNEKPLTPDMVKQLAYSLMSEEEAAVFEREREMNLGHPVPGLGS
FRVNVFWQRGSVGMVVRYILPSIPTVEELQLPPLLEELIKLKRGLILVVGSTGSGKSSTVAAMLQSRNRSMKGHILTIED
PIEFLFRHDQSIVNQREIGFDTLSYARALKNAMREAPDVLMIGEIRDPETLSQAIGYAQSGHLCISTLHGNNSYHMLNRV
MNFFPAEARGALLMDLSASLRAVISQRLVATLDGGRVPALELLINTPHISELIRAGEMDKIKEAIESSMSDGAQTFEQAL
FQLYQAGRISLDEALKNADSPTNLYWLINQSQEAGKGKDKPGPASAASFDSIKLDL

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=35.6495468277946, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789723, Length=159, Percent_Identity=42.7672955974843, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786296, Length=200, Percent_Identity=36.5, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41488; Mature: 41488

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMLPFFKLMAEKKASDLFLTSFSPPMLKLGGELTPVNEKPLTPDMVKQLAYSLMSEEEA
CCCCHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
AVFEREREMNLGHPVPGLGSFRVNVFWQRGSVGMVVRYILPSIPTVEELQLPPLLEELIK
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHH
LKRGLILVVGSTGSGKSSTVAAMLQSRNRSMKGHILTIEDPIEFLFRHDQSIVNQREIGF
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCH
DTLSYARALKNAMREAPDVLMIGEIRDPETLSQAIGYAQSGHLCISTLHGNNSYHMLNRV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHH
MNFFPAEARGALLMDLSASLRAVISQRLVATLDGGRVPALELLINTPHISELIRAGEMDK
HHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEHEEECCCCHHHHHHCCCHHH
IKEAIESSMSDGAQTFEQALFQLYQAGRISLDEALKNADSPTNLYWLINQSQEAGKGKDK
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
PGPASAASFDSIKLDL
CCCCCCCCCCCEEECC
>Mature Secondary Structure
MLMLPFFKLMAEKKASDLFLTSFSPPMLKLGGELTPVNEKPLTPDMVKQLAYSLMSEEEA
CCCCHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
AVFEREREMNLGHPVPGLGSFRVNVFWQRGSVGMVVRYILPSIPTVEELQLPPLLEELIK
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHH
LKRGLILVVGSTGSGKSSTVAAMLQSRNRSMKGHILTIEDPIEFLFRHDQSIVNQREIGF
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCH
DTLSYARALKNAMREAPDVLMIGEIRDPETLSQAIGYAQSGHLCISTLHGNNSYHMLNRV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHH
MNFFPAEARGALLMDLSASLRAVISQRLVATLDGGRVPALELLINTPHISELIRAGEMDK
HHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEHEEECCCCHHHHHHCCCHHH
IKEAIESSMSDGAQTFEQALFQLYQAGRISLDEALKNADSPTNLYWLINQSQEAGKGKDK
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
PGPASAASFDSIKLDL
CCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]