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The map label for this gene is ogt [H]

Identifier: 34496878

GI number: 34496878

Start: 1501655

End: 1502164

Strand: Reverse

Name: ogt [H]

Synonym: CV_1423

Alternate gene names: 34496878

Gene position: 1502164-1501655 (Counterclockwise)

Preceding gene: 34496879

Following gene: 34496877

Centisome position: 31.62

GC content: 72.16

Gene sequence:

>510_bases
ATGACAACCGCCGCCCGCCATGGCCTGATCGATACCCGTTTCGGCCCGGTGGCCGCCTGGCTGGACGCGCGCGGCGCGCT
GCTGCGCTTGTTGTTCAACCCCACGCCCGAACAGCTGGCCGCCCACGCTTGCCCGCGCGACGACGCGGCGCTGGCCGAGG
TGGCGCGCCAGCTGGCCGAATACCAGGCAGGAGATCGCCAAACCTTCGACATGCCGCTGGCGCCGCAAGGCACGCCGTTC
CAGCAGAAGGTATGGGCGGCGCTGCGCGAAATCCCCTATGGCCGCACCGTCAGCTACGGCGAGCTGGCGCTGGCGCTGGA
CTGCCCGGACGGCGCGCGGGCGGTGGGCCGTGCCAACGCCACCAATCCCATCGCGCTGATCGTGCCCTGCCACCGGGTAC
TGGGCGCCGATGGCAGCCTCACCGGCTATGCCGGCGGCCTGCCGCTGAAGGCCGCGCTGCTGCGTTTCGAGCTGGAGCGC
AGCGCGCCGCCGGGCTTGTTCGGCGGATAG

Upstream 100 bases:

>100_bases
ACGCCAAGCTGACGCTGCCGTCGGCGGCCTGATCCATATCGAATCCCCGCCGGGATGGCGCTACAATCGCGTCATCCCGC
TTCGTTTCAAGGCATACCCG

Downstream 100 bases:

>100_bases
GCCCGCTCGACCGCGTCTCTGCTGCAAACGGGTTTGCACACCGTCACCGCGCCTTCGTTCATCTTCCAGGCCATGCCAAA
CAAGGAATCATCATGCAACG

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MTTAARHGLIDTRFGPVAAWLDARGALLRLLFNPTPEQLAAHACPRDDAALAEVARQLAEYQAGDRQTFDMPLAPQGTPF
QQKVWAALREIPYGRTVSYGELALALDCPDGARAVGRANATNPIALIVPCHRVLGADGSLTGYAGGLPLKAALLRFELER
SAPPGLFGG

Sequences:

>Translated_169_residues
MTTAARHGLIDTRFGPVAAWLDARGALLRLLFNPTPEQLAAHACPRDDAALAEVARQLAEYQAGDRQTFDMPLAPQGTPF
QQKVWAALREIPYGRTVSYGELALALDCPDGARAVGRANATNPIALIVPCHRVLGADGSLTGYAGGLPLKAALLRFELER
SAPPGLFGG
>Mature_168_residues
TTAARHGLIDTRFGPVAAWLDARGALLRLLFNPTPEQLAAHACPRDDAALAEVARQLAEYQAGDRQTFDMPLAPQGTPFQ
QKVWAALREIPYGRTVSYGELALALDCPDGARAVGRANATNPIALIVPCHRVLGADGSLTGYAGGLPLKAALLRFELERS
APPGLFGG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=93, Percent_Identity=37.6344086021505, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=52.3809523809524, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=55.0561797752809, Blast_Score=99, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=45, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=45, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=47.0588235294118, Blast_Score=88, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17137554, Length=130, Percent_Identity=35.3846153846154, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17914; Mature: 17782

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAARHGLIDTRFGPVAAWLDARGALLRLLFNPTPEQLAAHACPRDDAALAEVARQLAE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHH
YQAGDRQTFDMPLAPQGTPFQQKVWAALREIPYGRTVSYGELALALDCPDGARAVGRANA
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHCCCCC
TNPIALIVPCHRVLGADGSLTGYAGGLPLKAALLRFELERSAPPGLFGG
CCCEEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTAARHGLIDTRFGPVAAWLDARGALLRLLFNPTPEQLAAHACPRDDAALAEVARQLAE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHH
YQAGDRQTFDMPLAPQGTPFQQKVWAALREIPYGRTVSYGELALALDCPDGARAVGRANA
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHCCCCC
TNPIALIVPCHRVLGADGSLTGYAGGLPLKAALLRFELERSAPPGLFGG
CCCEEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]