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The map label for this gene is dctA [H]

Identifier: 34496653

GI number: 34496653

Start: 1263766

End: 1264644

Strand: Reverse

Name: dctA [H]

Synonym: CV_1198

Alternate gene names: 34496653

Gene position: 1264644-1263766 (Counterclockwise)

Preceding gene: 34496655

Following gene: 34496648

Centisome position: 26.62

GC content: 63.37

Gene sequence:

>879_bases
ATGCACGCCGAATCCGGCATCAAGGCCAGCGCCATTTCGCTGAAGGACTTCGTCACCCACGCCATCCCCAAGAGCGTGTT
CGAGGCGATGGCCAACAACGAGATCCTGCAGATCGTGATCTTCTCGGTGTTCTTCGGCAGCGCGATGGCCGCGCTGGGCG
AACGCGCCAAGGCGCTGATCGACGTGATCGACGTCGTCGCCCACGTGATGCTGAAGGTCACCAGCTATGTGATGAACTTC
GCGCCGCTGGCGGTGTTCGGCGCCATCGCCGCCACCGTGGCCAAGGAAGGCCTGTCCATCCTCGGCACCTATGGCAAGTT
CATGGCGCAGTTCTATTTCTCCATCGGCATCCTGTGGGCGCTGCTGATCGCCGTCGGCGTGCTGATCGTCGGCCCGCGCC
TGTTGCACCTGATGGGCATGATCAAGGAACCGCTGCTGCTGTCGTTCACCACCGCCAGCTCCGAAGCCGCCTATCCGAAA
ACCCTGGAACAGCTGGAGCGCTTCGGCGTGTCCAACAAGATCGCCAGCTTCGTGCTGCCGATGGGCTACTCGTTCAACCT
GGACGGCTCGATGATGTACTGCACCTTCGCGGTGATCTTCATCGCCCAGGCCTACGGCATCGACCTGACGCTGGCGCAGG
AAATCTCCATGCTGCTGATCCTGATGCTGACCTCCAAGGGCATGGCCGGCGTGCCGCGCGCGTCGCTGGTGGTGATCGCC
GCCACCCTGGCGCAGTTCAACATCCCGGAAGCCGGCCTGCTGCTGCTGCTGGGCATCGACCACTTCCTGGACATGGGCCG
CTCCGCCACCAACGTGGTGGGCAACTCCATCGCCACCGCCGTGGTGGCCAAGTGGGAAGGCGAGTTGAAGCGCCACTGA

Upstream 100 bases:

>100_bases
ATGGGCTGGTTCATCCGCCTCGCTGGTGTCGCTGACCCTGGGCCTGATCATGGTGAACATCCTGAAGCCGGGCGTGGCGC
TGAACCTGCCGCTGCCGGAC

Downstream 100 bases:

>100_bases
GCCCGCTTCGCCCCGCGCCAACGCCACCCTTCGCGGTGGCGTTTTTCATGGGCGGAAAACTGCAAGAAACTATTGACAGT
ACGGGCTCGCACCGACATAA

Product: proton/sodium-glutamate symport protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MHAESGIKASAISLKDFVTHAIPKSVFEAMANNEILQIVIFSVFFGSAMAALGERAKALIDVIDVVAHVMLKVTSYVMNF
APLAVFGAIAATVAKEGLSILGTYGKFMAQFYFSIGILWALLIAVGVLIVGPRLLHLMGMIKEPLLLSFTTASSEAAYPK
TLEQLERFGVSNKIASFVLPMGYSFNLDGSMMYCTFAVIFIAQAYGIDLTLAQEISMLLILMLTSKGMAGVPRASLVVIA
ATLAQFNIPEAGLLLLLGIDHFLDMGRSATNVVGNSIATAVVAKWEGELKRH

Sequences:

>Translated_292_residues
MHAESGIKASAISLKDFVTHAIPKSVFEAMANNEILQIVIFSVFFGSAMAALGERAKALIDVIDVVAHVMLKVTSYVMNF
APLAVFGAIAATVAKEGLSILGTYGKFMAQFYFSIGILWALLIAVGVLIVGPRLLHLMGMIKEPLLLSFTTASSEAAYPK
TLEQLERFGVSNKIASFVLPMGYSFNLDGSMMYCTFAVIFIAQAYGIDLTLAQEISMLLILMLTSKGMAGVPRASLVVIA
ATLAQFNIPEAGLLLLLGIDHFLDMGRSATNVVGNSIATAVVAKWEGELKRH
>Mature_292_residues
MHAESGIKASAISLKDFVTHAIPKSVFEAMANNEILQIVIFSVFFGSAMAALGERAKALIDVIDVVAHVMLKVTSYVMNF
APLAVFGAIAATVAKEGLSILGTYGKFMAQFYFSIGILWALLIAVGVLIVGPRLLHLMGMIKEPLLLSFTTASSEAAYPK
TLEQLERFGVSNKIASFVLPMGYSFNLDGSMMYCTFAVIFIAQAYGIDLTLAQEISMLLILMLTSKGMAGVPRASLVVIA
ATLAQFNIPEAGLLLLLGIDHFLDMGRSATNVVGNSIATAVVAKWEGELKRH

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=268, Percent_Identity=30.5970149253731, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4827012, Length=267, Percent_Identity=29.5880149812734, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI66773030, Length=255, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI40254478, Length=270, Percent_Identity=29.2592592592593, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI194239697, Length=261, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI21314632, Length=259, Percent_Identity=26.2548262548263, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI223468564, Length=256, Percent_Identity=26.171875, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI223468566, Length=256, Percent_Identity=26.171875, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI5032093, Length=258, Percent_Identity=26.7441860465116, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI262359914, Length=202, Percent_Identity=29.2079207920792, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI301601644, Length=170, Percent_Identity=27.0588235294118, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1790514, Length=273, Percent_Identity=44.3223443223443, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1789947, Length=277, Percent_Identity=38.6281588447653, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788024, Length=299, Percent_Identity=27.7591973244147, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789473, Length=227, Percent_Identity=23.7885462555066, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71996953, Length=279, Percent_Identity=28.673835125448, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17537407, Length=259, Percent_Identity=28.1853281853282, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=276, Percent_Identity=28.9855072463768, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193206505, Length=255, Percent_Identity=30.5882352941176, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983106, Length=281, Percent_Identity=27.7580071174377, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71983099, Length=281, Percent_Identity=27.7580071174377, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=283, Percent_Identity=26.1484098939929, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24583025, Length=256, Percent_Identity=28.125, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI17137668, Length=256, Percent_Identity=28.125, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24583023, Length=256, Percent_Identity=28.125, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI281360481, Length=297, Percent_Identity=28.2828282828283, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281360483, Length=297, Percent_Identity=28.2828282828283, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137666, Length=297, Percent_Identity=28.2828282828283, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 31287; Mature: 31287

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAESGIKASAISLKDFVTHAIPKSVFEAMANNEILQIVIFSVFFGSAMAALGERAKALI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVIDVVAHVMLKVTSYVMNFAPLAVFGAIAATVAKEGLSILGTYGKFMAQFYFSIGILWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIAVGVLIVGPRLLHLMGMIKEPLLLSFTTASSEAAYPKTLEQLERFGVSNKIASFVLP
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHC
MGYSFNLDGSMMYCTFAVIFIAQAYGIDLTLAQEISMLLILMLTSKGMAGVPRASLVVIA
CCCEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ATLAQFNIPEAGLLLLLGIDHFLDMGRSATNVVGNSIATAVVAKWEGELKRH
HHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHAESGIKASAISLKDFVTHAIPKSVFEAMANNEILQIVIFSVFFGSAMAALGERAKALI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVIDVVAHVMLKVTSYVMNFAPLAVFGAIAATVAKEGLSILGTYGKFMAQFYFSIGILWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIAVGVLIVGPRLLHLMGMIKEPLLLSFTTASSEAAYPKTLEQLERFGVSNKIASFVLP
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHC
MGYSFNLDGSMMYCTFAVIFIAQAYGIDLTLAQEISMLLILMLTSKGMAGVPRASLVVIA
CCCEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ATLAQFNIPEAGLLLLLGIDHFLDMGRSATNVVGNSIATAVVAKWEGELKRH
HHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA