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The map label for this gene is eutB [H]

Identifier: 34496640

GI number: 34496640

Start: 1247654

End: 1249036

Strand: Reverse

Name: eutB [H]

Synonym: CV_1185

Alternate gene names: 34496640

Gene position: 1249036-1247654 (Counterclockwise)

Preceding gene: 34496641

Following gene: 34496639

Centisome position: 26.29

GC content: 69.2

Gene sequence:

>1383_bases
ATGAGCCACGCCATCGCCATAGGCCCGCGAACCTACCGCTTCGCCGACTTGAAAACGCTGCTGGCGCGCGCCAGTCCCCT
GCGCTCCGGCGACCAGCTGGCCGGCGTCGCCGCCGCCAGCGCCGAAGAGCGGGTGGCGGCGCAGATGTGCCTGGCCGACT
TGCCGCTGAAGGTCTTTCTCAACCAGGCGGTGATTCCGTACGAGGAGGACGAGGTCACCCGGCTGATCCTGGACAGCCAT
GACCAGGCCGCCTTCGCCGCCGTATCGCACCTGACCGTCGGCGGCCTGCGCGACTGGCTGCTGTCCGACGCCGCCACCGC
GGAAACGCTGGCGGCGCTGGCGCCCGGCCTCACGCCGGAAATGGCGGCGGCGGTGTCCAAGCTGATGCGCAACCAGGACC
TGGTGCTGGCGGCGAGGAAATGCCGCGTCGTCACCGCCTTCCGCAACACCCAGGGACTGCCCGGCCGCATGGGCGTGCGG
CTGCAGCCCAATCATCCGGCCGACGACGCGCGCGCCATCGCCGCCGCGACGCTGGACGGCCTGCTGTACGGCGCCGGCGA
CGCGGTGATCGGCATCAACCCGGCCGGCGACAGCCCGGACGACATCCGGAGCTTGCTGACGCTGCTGGACGAGGTGCGCC
AGCGCTACGCCATCCCTACCCAGAGCTGCGTGCTCACCCACGTCACCAGCACGCTGGACCTGATGCGCCGGGGCGCGCCG
GTGGATCTGGTGTTCCAGTCCATCGCCGGCACTCAGAAGGCCAACGCCGGCTTCGGCGTCAGCCTCGCCGTGCTGGACGA
GGCGCATCAGGCGGCGCGGGAGCTGAAGCGCGGCACGGTGGGCGACAATGTGATGTATTTCGAAACCGGCCAGGGCAGCG
CGCTGTCGGCCAACGCCCACTGGGGCGTGGACCAACAGACCTGCGAGGCGCGCGCCTACGCGGTGGCGCGGCGCTACTCG
CCCTTGCTGGTCAACACCGTGGTCGGCTTCATCGGCCCGGAATACCTGTACAACGGCAAGCAGATCATCCGCGCCGGACT
GGAAGACCATTTCTGCGGCAAGCTGCTGGGCCTGCCGATGGGCTGCGACATCTGCTACACCAACCACGCCGAAGCCGACC
AGGACGATATGGACGCGCTGTTGACGCTGCTGGGCGCGGCCGGCGTGCATTTCATCATCGGCGTGCCCGGCGCCGACGAC
ATCATGCTGGGCTATCAGAGCACCTCCTTCCACGACGCGCTCTACCTGCGCAATGTGCTGGGACTCAAGCGCGCGCCGGA
GTTCGAAGACTGGCTGGAAACGATGGAGATCACCCGCGGCGGCAGGCTGCTGCCGCAGGACAGGCGGCAACGGCTGCTGG
CGATGATGGAGGGCGTGGCGTGA

Upstream 100 bases:

>100_bases
TCGCCACCGCCCGCCAGCGCGCCGCCGCCGCCGACGACGCGCAGCTGCAAGGTTTGGCCGAGCCGCCAGCCGAGCCAGCC
CCCTCCGTCCGGAGTCCGAC

Downstream 100 bases:

>100_bases
AAGAAAAGCAAAATCCCGTCACCGAAGACGGCTGGGGCGAGCTGTCCCGCCTCACCGCGGCTCGCATCGCGCTGGGCCGC
GCCGGCAGCAGCCTGCCCTG

Product: ethanolamine ammonia-lyase heavy chain

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSHAIAIGPRTYRFADLKTLLARASPLRSGDQLAGVAAASAEERVAAQMCLADLPLKVFLNQAVIPYEEDEVTRLILDSH
DQAAFAAVSHLTVGGLRDWLLSDAATAETLAALAPGLTPEMAAAVSKLMRNQDLVLAARKCRVVTAFRNTQGLPGRMGVR
LQPNHPADDARAIAAATLDGLLYGAGDAVIGINPAGDSPDDIRSLLTLLDEVRQRYAIPTQSCVLTHVTSTLDLMRRGAP
VDLVFQSIAGTQKANAGFGVSLAVLDEAHQAARELKRGTVGDNVMYFETGQGSALSANAHWGVDQQTCEARAYAVARRYS
PLLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGLPMGCDICYTNHAEADQDDMDALLTLLGAAGVHFIIGVPGADD
IMLGYQSTSFHDALYLRNVLGLKRAPEFEDWLETMEITRGGRLLPQDRRQRLLAMMEGVA

Sequences:

>Translated_460_residues
MSHAIAIGPRTYRFADLKTLLARASPLRSGDQLAGVAAASAEERVAAQMCLADLPLKVFLNQAVIPYEEDEVTRLILDSH
DQAAFAAVSHLTVGGLRDWLLSDAATAETLAALAPGLTPEMAAAVSKLMRNQDLVLAARKCRVVTAFRNTQGLPGRMGVR
LQPNHPADDARAIAAATLDGLLYGAGDAVIGINPAGDSPDDIRSLLTLLDEVRQRYAIPTQSCVLTHVTSTLDLMRRGAP
VDLVFQSIAGTQKANAGFGVSLAVLDEAHQAARELKRGTVGDNVMYFETGQGSALSANAHWGVDQQTCEARAYAVARRYS
PLLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGLPMGCDICYTNHAEADQDDMDALLTLLGAAGVHFIIGVPGADD
IMLGYQSTSFHDALYLRNVLGLKRAPEFEDWLETMEITRGGRLLPQDRRQRLLAMMEGVA
>Mature_459_residues
SHAIAIGPRTYRFADLKTLLARASPLRSGDQLAGVAAASAEERVAAQMCLADLPLKVFLNQAVIPYEEDEVTRLILDSHD
QAAFAAVSHLTVGGLRDWLLSDAATAETLAALAPGLTPEMAAAVSKLMRNQDLVLAARKCRVVTAFRNTQGLPGRMGVRL
QPNHPADDARAIAAATLDGLLYGAGDAVIGINPAGDSPDDIRSLLTLLDEVRQRYAIPTQSCVLTHVTSTLDLMRRGAPV
DLVFQSIAGTQKANAGFGVSLAVLDEAHQAARELKRGTVGDNVMYFETGQGSALSANAHWGVDQQTCEARAYAVARRYSP
LLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGLPMGCDICYTNHAEADQDDMDALLTLLGAAGVHFIIGVPGADDI
MLGYQSTSFHDALYLRNVLGLKRAPEFEDWLETMEITRGGRLLPQDRRQRLLAMMEGVA

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=434, Percent_Identity=51.6129032258064, Blast_Score=459, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 49431; Mature: 49300

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHAIAIGPRTYRFADLKTLLARASPLRSGDQLAGVAAASAEERVAAQMCLADLPLKVFL
CCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NQAVIPYEEDEVTRLILDSHDQAAFAAVSHLTVGGLRDWLLSDAATAETLAALAPGLTPE
CCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAAAVSKLMRNQDLVLAARKCRVVTAFRNTQGLPGRMGVRLQPNHPADDARAIAAATLDG
HHHHHHHHHCCCCCEEEHHHHEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
LLYGAGDAVIGINPAGDSPDDIRSLLTLLDEVRQRYAIPTQSCVLTHVTSTLDLMRRGAP
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
VDLVFQSIAGTQKANAGFGVSLAVLDEAHQAARELKRGTVGDNVMYFETGQGSALSANAH
HHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCC
WGVDQQTCEARAYAVARRYSPLLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGLPM
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCCC
GCDICYTNHAEADQDDMDALLTLLGAAGVHFIIGVPGADDIMLGYQSTSFHDALYLRNVL
CCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
GLKRAPEFEDWLETMEITRGGRLLPQDRRQRLLAMMEGVA
HCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHAIAIGPRTYRFADLKTLLARASPLRSGDQLAGVAAASAEERVAAQMCLADLPLKVFL
CCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NQAVIPYEEDEVTRLILDSHDQAAFAAVSHLTVGGLRDWLLSDAATAETLAALAPGLTPE
CCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAAAVSKLMRNQDLVLAARKCRVVTAFRNTQGLPGRMGVRLQPNHPADDARAIAAATLDG
HHHHHHHHHCCCCCEEEHHHHEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
LLYGAGDAVIGINPAGDSPDDIRSLLTLLDEVRQRYAIPTQSCVLTHVTSTLDLMRRGAP
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
VDLVFQSIAGTQKANAGFGVSLAVLDEAHQAARELKRGTVGDNVMYFETGQGSALSANAH
HHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCC
WGVDQQTCEARAYAVARRYSPLLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGLPM
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCCC
GCDICYTNHAEADQDDMDALLTLLGAAGVHFIIGVPGADDIMLGYQSTSFHDALYLRNVL
CCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
GLKRAPEFEDWLETMEITRGGRLLPQDRRQRLLAMMEGVA
HCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]