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The map label for this gene is ubiF [H]

Identifier: 34496587

GI number: 34496587

Start: 1185671

End: 1186852

Strand: Reverse

Name: ubiF [H]

Synonym: CV_1132

Alternate gene names: 34496587

Gene position: 1186852-1185671 (Counterclockwise)

Preceding gene: 34496588

Following gene: 34496586

Centisome position: 24.98

GC content: 71.91

Gene sequence:

>1182_bases
ATGGAACACCGCGACATCGTCATCGTCGGCGGCGGCATGGTGGGCAGCGCGCTGGGCGCGGCGCTCTCGGGCAAGGGCCT
GTCGGTCTCTGTGCTGGAACGGGAAGCCCCCGCGCCGTTCGCGCCGGAACAGGCGCCGGACCTGCGGGTCTCGGCGATCA
GCCCGGCTTCCGCCGCCTTTCTGCAAACCATCGGCGCCTGGGGCCAGGCGCTGGCGATGCGCGCCGCGCCCTATCGCCGC
ATGCAGGTCTGGGAGCGGGACGAGGCCGCCGGCACGCTGTTCGACGCCGCCGAGGCCGGCGTGCCCGAACTGGGCCACAT
CGTGGAAAACCGCGTGGTGCAGCTGGCCGCCACCGAGGCCTTGCTGGCCAAGGGCGACATCGACTACCGCTGCCCGGCCG
TGGTGTCGGACATCGTCTACCGTCCCCAAGCCTCCCAGTTGACGCTGTCGGACGGCAGCAGGCTGCAAGCCAGGCTGCTG
ATCGCCGCCGACGGCGCCCGCTCTCAGGTGCGCGACGCCGCCGGCATCGGCGTCACCGGCTGGGACTACCCGATGCACGC
GCTGGTCCTGTCCTGCCGGATCGACGGCGGCCAGCAGGACATCACCTGGCAGCGCTTCACGCCGGACGGCCCGCAGGCCT
TCCTGCCGCTAAGCGGCGACGCGGCCTCCCTGGTGTGGTACCACCGGCCCGAAGAGGTGAAGCGCTTGCTGACGCTGGGC
GACGACGAGCTGATCGCCGCCGTCAGCCAAGCGTTTCCGCGCAAGCTGCCGGGCATCCGGGAGCTGGCGGCGCGCGGCAG
CTTCGGCCTGACCCGCCGCCACGCCCAATGCTACGTCAAGGACGGCGTGGCGCTGGCCGGCGACGCCGCCCACACCATCC
ACCCGCTGGCAGGCCAGGGCGTCAACCTGGGCTTCCAGGACGCAGCGATGCTGGCCCGGACCATTCTGGACGCCCATGCC
AAGGGAGAGGATTGGGCGAGCGGGAAGACGCTGGTGCGCTATCAGCGCGCGCGCAAGCCCGCCAACCTGGCGATGCAGGC
CGGCATGGACGCCTTCTGCTACGGCTTTGCCAACGATATCGGCCCGCTGCGCCTGCTGCGCGGCCTGGGCTTGCGGCTGG
CAGACAAGGCCGGGCCGCTGAAGCGCGAGGCGATACGCTACGCGCTGGGGCTGCGCGCCTGA

Upstream 100 bases:

>100_bases
GGAGGCAGGCATTGATCCAGGCCAAGGCGGCATCCGGCCGTCATCGCCAGAATGAGCGCCATTCGCGCCGGCTCAAGGCC
GGCCTGACAAGGAGAATGCA

Downstream 100 bases:

>100_bases
GCGTTTCTCCCCTTCATGCTAGGATCGGGGGACTTCCCCCTCTCCTGGCCTATCCATGAACACATTCCGCATTCGCGCCG
CCATCGGTCTGCTGCAAGGG

Product: monooxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MEHRDIVIVGGGMVGSALGAALSGKGLSVSVLEREAPAPFAPEQAPDLRVSAISPASAAFLQTIGAWGQALAMRAAPYRR
MQVWERDEAAGTLFDAAEAGVPELGHIVENRVVQLAATEALLAKGDIDYRCPAVVSDIVYRPQASQLTLSDGSRLQARLL
IAADGARSQVRDAAGIGVTGWDYPMHALVLSCRIDGGQQDITWQRFTPDGPQAFLPLSGDAASLVWYHRPEEVKRLLTLG
DDELIAAVSQAFPRKLPGIRELAARGSFGLTRRHAQCYVKDGVALAGDAAHTIHPLAGQGVNLGFQDAAMLARTILDAHA
KGEDWASGKTLVRYQRARKPANLAMQAGMDAFCYGFANDIGPLRLLRGLGLRLADKAGPLKREAIRYALGLRA

Sequences:

>Translated_393_residues
MEHRDIVIVGGGMVGSALGAALSGKGLSVSVLEREAPAPFAPEQAPDLRVSAISPASAAFLQTIGAWGQALAMRAAPYRR
MQVWERDEAAGTLFDAAEAGVPELGHIVENRVVQLAATEALLAKGDIDYRCPAVVSDIVYRPQASQLTLSDGSRLQARLL
IAADGARSQVRDAAGIGVTGWDYPMHALVLSCRIDGGQQDITWQRFTPDGPQAFLPLSGDAASLVWYHRPEEVKRLLTLG
DDELIAAVSQAFPRKLPGIRELAARGSFGLTRRHAQCYVKDGVALAGDAAHTIHPLAGQGVNLGFQDAAMLARTILDAHA
KGEDWASGKTLVRYQRARKPANLAMQAGMDAFCYGFANDIGPLRLLRGLGLRLADKAGPLKREAIRYALGLRA
>Mature_393_residues
MEHRDIVIVGGGMVGSALGAALSGKGLSVSVLEREAPAPFAPEQAPDLRVSAISPASAAFLQTIGAWGQALAMRAAPYRR
MQVWERDEAAGTLFDAAEAGVPELGHIVENRVVQLAATEALLAKGDIDYRCPAVVSDIVYRPQASQLTLSDGSRLQARLL
IAADGARSQVRDAAGIGVTGWDYPMHALVLSCRIDGGQQDITWQRFTPDGPQAFLPLSGDAASLVWYHRPEEVKRLLTLG
DDELIAAVSQAFPRKLPGIRELAARGSFGLTRRHAQCYVKDGVALAGDAAHTIHPLAGQGVNLGFQDAAMLARTILDAHA
KGEDWASGKTLVRYQRARKPANLAMQAGMDAFCYGFANDIGPLRLLRGLGLRLADKAGPLKREAIRYALGLRA

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=386, Percent_Identity=35.4922279792746, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI32967307, Length=352, Percent_Identity=33.8068181818182, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1786883, Length=387, Percent_Identity=48.578811369509, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=42.7480916030534, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1789274, Length=389, Percent_Identity=31.1053984575836, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25146631, Length=433, Percent_Identity=31.4087759815243, Blast_Score=185, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321694, Length=453, Percent_Identity=27.8145695364238, Blast_Score=129, Evalue=5e-31,
Organism=Drosophila melanogaster, GI19920760, Length=438, Percent_Identity=33.7899543378995, Blast_Score=185, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 41925; Mature: 41925

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHRDIVIVGGGMVGSALGAALSGKGLSVSVLEREAPAPFAPEQAPDLRVSAISPASAAF
CCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHH
LQTIGAWGQALAMRAAPYRRMQVWERDEAAGTLFDAAEAGVPELGHIVENRVVQLAATEA
HHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LLAKGDIDYRCPAVVSDIVYRPQASQLTLSDGSRLQARLLIAADGARSQVRDAAGIGVTG
HHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEEEEEEECCCHHHHHHHCCCCCCC
WDYPMHALVLSCRIDGGQQDITWQRFTPDGPQAFLPLSGDAASLVWYHRPEEVKRLLTLG
CCCHHHHEEEEEEECCCCCCCEEEEECCCCCCEEEECCCCCEEEEEEECHHHHHHHHHCC
DDELIAAVSQAFPRKLPGIRELAARGSFGLTRRHAQCYVKDGVALAGDAAHTIHPLAGQG
CHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHEEEEECCEEEECCCHHHCCCCCCCC
VNLGFQDAAMLARTILDAHAKGEDWASGKTLVRYQRARKPANLAMQAGMDAFCYGFANDI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCC
GPLRLLRGLGLRLADKAGPLKREAIRYALGLRA
HHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MEHRDIVIVGGGMVGSALGAALSGKGLSVSVLEREAPAPFAPEQAPDLRVSAISPASAAF
CCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHH
LQTIGAWGQALAMRAAPYRRMQVWERDEAAGTLFDAAEAGVPELGHIVENRVVQLAATEA
HHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LLAKGDIDYRCPAVVSDIVYRPQASQLTLSDGSRLQARLLIAADGARSQVRDAAGIGVTG
HHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEEEEEEECCCHHHHHHHCCCCCCC
WDYPMHALVLSCRIDGGQQDITWQRFTPDGPQAFLPLSGDAASLVWYHRPEEVKRLLTLG
CCCHHHHEEEEEEECCCCCCCEEEEECCCCCCEEEECCCCCEEEEEEECHHHHHHHHHCC
DDELIAAVSQAFPRKLPGIRELAARGSFGLTRRHAQCYVKDGVALAGDAAHTIHPLAGQG
CHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHEEEEECCEEEECCCHHHCCCCCCCC
VNLGFQDAAMLARTILDAHAKGEDWASGKTLVRYQRARKPANLAMQAGMDAFCYGFANDI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCC
GPLRLLRGLGLRLADKAGPLKREAIRYALGLRA
HHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]