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The map label for this gene is pitA [H]

Identifier: 34496389

GI number: 34496389

Start: 968831

End: 970285

Strand: Reverse

Name: pitA [H]

Synonym: CV_0934

Alternate gene names: 34496389

Gene position: 970285-968831 (Counterclockwise)

Preceding gene: 34496390

Following gene: 34496384

Centisome position: 20.42

GC content: 66.12

Gene sequence:

>1455_bases
ATGCTGGACCTGTTTTCCGGGCTCGATACCCATGTAGCCATCACGCTGATGCTTTCGCTGGGCTTCGTGCTCGCATTTGA
GTTCATCAACGGTTTTCACGACACCGCCAACGCCGTCGCCACCGTCATCTACACCCAGTCGATGAAGCCGCGCCTCGCCG
TGTTCTATTCCGGCGTCTGCAACTTCCTCGGCGTGATGAGCGGCGGCTTGGCCGTCGCCTACGCCATCGTCCACCTGCTG
CCGGTGGATCTGTTGATCTCGGTGAACACCAGCCGCGGCATGGCCATGGTATTCGCGCTGCTCGCCGCCGCCATCCTGTG
GAACCTGGGCACCTGGTATCTGGGCCTGCCCGCCTCGTCCAGCCACACGCTGATCGGCGCCATCCTGGGCGTCGGCGTGG
CCAACAGCTTCATCAACCACACCTCGCTGTCCCACGGCATCAACTGGGGCAAGGCAATCGACGTCGGCGCCTCGCTGTTG
GTGTCGCCGATCGCCGGCGCGGTGATGGCCGGCCTGATCGTCATCCTGCTGCGGCGCTTCCGCCCGCGCAGCAACGTCCA
CAAGACGCCGTACCAGCGCCAGCAAGTCGAAGGCCGCAAGCATCCGCCGTTCTGGACCCGCTTCATGCTGATCGTGTCGG
CGATGGGCGTCAGCTTCGCCCACGGCTCCAACGACGGCCAGAAGGGCGTCGGCCTGGTGATGCTGGTGCTGATCGGCATC
GTGCCGGCCAAGTTCGTGCTGAACCTGGACAGCACGCCGTACCAGCTGGAGCGCACCCGCGACGCGGCGCAGCACCTGCA
GCAGTTCTACCAGCGCCATGAGGCCGACCTGTCCAAGATCATCGTCAAGTCGAACAGCACCAGCCAGTACGAGTGCCACC
CGCAGCAGACCGTGGCCACCGCCGACCAGCTGCTGAAACTGATGGGCGACGGCAACGATTACCACACCCTCAGCGCCTCG
CAGCGCTGGGACGTGCGCACCCAGCTCCTGTGCCTGGACGACGCCGCCAAGAAGGCCGCCGACCTGCCCAAGCTGTCGTC
CGACGACAAGCAGCTGCTGAGCAATCTGCGCAAGGACCTGACCACCACCACCGAATACGCGCCCACCTGGGTGATCGTCG
CCGTGGCCTTGGCGCTGGGCATCGGCACCATGATAGGCTGGCGCCGCGTGGTGAAGACCGTGGGCGAAGGCATAGGCAAG
AAGGACATGACCTACGCCCAGGGCGTGGCCGCGCAGACCACCGCCGCCGTCTCCATCGGCCTGGCCAGCTGGTTCGGCTT
CCCGGTGTCCACCACCCATGTGCTGTCCAGCGGCGTGGCCGGCACCATGATCGCCGACAAGGCCGGCCTGCAGATGAGCA
CCGTGCGCTCCATCGCGCTGGCCTGGCTGTTCACGCTGCCGGCCTCGATGGCCCTGGCCGCCGGCCTGTACTACCTGGCG
GCGCAGTGGTTCTGA

Upstream 100 bases:

>100_bases
CGCAGGCAAAACCACACGCTTTGTTTTTTTTTTATAAAGACTTGACGGCCATGGCGCGGAACACAACAATCCCGCCTTTA
CTGATAAACGCTGACTCGCC

Downstream 100 bases:

>100_bases
CCCAAACCGCGCCCAAACGCAAAAGCCGCTCGATGAGCGGCTTTTTTCTTGCTTGATCCAGGTCAACGGAGCGGCTGTCC
GCGCTTGAACGAAAAATTCA

Product: low-affinity inorganic phosphate transporter 1

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MLDLFSGLDTHVAITLMLSLGFVLAFEFINGFHDTANAVATVIYTQSMKPRLAVFYSGVCNFLGVMSGGLAVAYAIVHLL
PVDLLISVNTSRGMAMVFALLAAAILWNLGTWYLGLPASSSHTLIGAILGVGVANSFINHTSLSHGINWGKAIDVGASLL
VSPIAGAVMAGLIVILLRRFRPRSNVHKTPYQRQQVEGRKHPPFWTRFMLIVSAMGVSFAHGSNDGQKGVGLVMLVLIGI
VPAKFVLNLDSTPYQLERTRDAAQHLQQFYQRHEADLSKIIVKSNSTSQYECHPQQTVATADQLLKLMGDGNDYHTLSAS
QRWDVRTQLLCLDDAAKKAADLPKLSSDDKQLLSNLRKDLTTTTEYAPTWVIVAVALALGIGTMIGWRRVVKTVGEGIGK
KDMTYAQGVAAQTTAAVSIGLASWFGFPVSTTHVLSSGVAGTMIADKAGLQMSTVRSIALAWLFTLPASMALAAGLYYLA
AQWF

Sequences:

>Translated_484_residues
MLDLFSGLDTHVAITLMLSLGFVLAFEFINGFHDTANAVATVIYTQSMKPRLAVFYSGVCNFLGVMSGGLAVAYAIVHLL
PVDLLISVNTSRGMAMVFALLAAAILWNLGTWYLGLPASSSHTLIGAILGVGVANSFINHTSLSHGINWGKAIDVGASLL
VSPIAGAVMAGLIVILLRRFRPRSNVHKTPYQRQQVEGRKHPPFWTRFMLIVSAMGVSFAHGSNDGQKGVGLVMLVLIGI
VPAKFVLNLDSTPYQLERTRDAAQHLQQFYQRHEADLSKIIVKSNSTSQYECHPQQTVATADQLLKLMGDGNDYHTLSAS
QRWDVRTQLLCLDDAAKKAADLPKLSSDDKQLLSNLRKDLTTTTEYAPTWVIVAVALALGIGTMIGWRRVVKTVGEGIGK
KDMTYAQGVAAQTTAAVSIGLASWFGFPVSTTHVLSSGVAGTMIADKAGLQMSTVRSIALAWLFTLPASMALAAGLYYLA
AQWF
>Mature_484_residues
MLDLFSGLDTHVAITLMLSLGFVLAFEFINGFHDTANAVATVIYTQSMKPRLAVFYSGVCNFLGVMSGGLAVAYAIVHLL
PVDLLISVNTSRGMAMVFALLAAAILWNLGTWYLGLPASSSHTLIGAILGVGVANSFINHTSLSHGINWGKAIDVGASLL
VSPIAGAVMAGLIVILLRRFRPRSNVHKTPYQRQQVEGRKHPPFWTRFMLIVSAMGVSFAHGSNDGQKGVGLVMLVLIGI
VPAKFVLNLDSTPYQLERTRDAAQHLQQFYQRHEADLSKIIVKSNSTSQYECHPQQTVATADQLLKLMGDGNDYHTLSAS
QRWDVRTQLLCLDDAAKKAADLPKLSSDDKQLLSNLRKDLTTTTEYAPTWVIVAVALALGIGTMIGWRRVVKTVGEGIGK
KDMTYAQGVAAQTTAAVSIGLASWFGFPVSTTHVLSSGVAGTMIADKAGLQMSTVRSIALAWLFTLPASMALAAGLYYLA
AQWF

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=499, Percent_Identity=54.3086172344689, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1789360, Length=497, Percent_Identity=53.9235412474849, Blast_Score=524, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI25146401, Length=494, Percent_Identity=25.1012145748988, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32566716, Length=506, Percent_Identity=24.1106719367589, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71981576, Length=485, Percent_Identity=24.3298969072165, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 52140; Mature: 52140

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCC
>Mature Secondary Structure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HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]