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The map label for this gene is maeB [H]

Identifier: 34496371

GI number: 34496371

Start: 947206

End: 949491

Strand: Reverse

Name: maeB [H]

Synonym: CV_0916

Alternate gene names: 34496371

Gene position: 949491-947206 (Counterclockwise)

Preceding gene: 34496372

Following gene: 34496370

Centisome position: 19.98

GC content: 65.66

Gene sequence:

>2286_bases
ATGGACGAGCAATTGCGCCAAAGCGCACTGGAGTTTCACCAGTTCCCGACACCGGGCAAGATTCAGGTTGCCCCGACCAA
GCCGCTGACCACCCAGCGCGATCTGGCACTGGCCTACTCCCCCGGCGTGACCGCCCCGTGCGACGCCATCGTCGAAGACC
CGCTCAACGCCTACAAATACACCGCCCGCGGCAACCTGGTGGCCGTGATTTCCAACGGCACCGCTGTGCTCGGCCTGGGC
AACATCGGCGCGCTGGCCAGCAAGCCGGTGATGGAAGGCAAGGGCGTATTGTTCAAGAAGTTCGCCGGCATCGACGTGTT
CGACATCGAGGTCAACGAGCTCGATCCCGACAAGCTGGTCGACATCATCTGCTCGCTGGAGCCGACCTTCGGCGGCATCA
ACCTGGAAGACATCAAGGCGCCGGAGTGCTTCTACATCGAGAAGAAGTGCCGCGAGCGCATGGGCATTCCGGTCTTCCAC
GACGACCAGCACGGCACCGCCATCGTGGCGGCCGCGGCTGTGCTGAACGGCCTGCGCCTGGTGAAAAAGGAAATCGGCGA
AGTGCGCGTGGTGGCCTCCGGCGCCGGCGCCGCCGCCATCGCCTGCCTGGACCTGTTGGTGGCCCTGGGCGTGAAGCGCG
AAAACATCACCATCTGCGACTCCAAGGGCGTGGTCTTCGTCGGCCGCGACGAGAAGCTGGACGAATCCAAGCTGCGCTAC
GCCATCCAGGACAACGGCTGGCGCAAGCTGGCCGACGCGATGGCCGGCGCCGACATCTTCATGGGTCTGTCCGGCCCGCG
CCTGGTGAGCCAGGACATGGTGAAGAGCATGGCCCGCGATCCGCTGATCCTGGCCATGGCCAACCCGGAACCGGAAATCC
TGCCGCCGCTGGTCAAGGAAGTGCGCCCGGACGCCATCATCGGCACCGGCCGCTCGGACTTCCCGAACCAGGTCAACAAC
GTGCTGTGCTTCCCCTTCATCTTCCGCGGCGCGCTGGACGTGGGCGCGACCACCATCAATGAAGAGATGAAGCTGGCCAC
CGTGCGCGCGATCGCCGACCTGGCGATGGCCGAGCAGAACGACGTGGTCGCCTCCGCCTACGGCGACCAGGAGCTGTCCT
TCGGTCCGGAATACGTGATTCCGAAACCGTTCGACCCGCGCCTGATCGTCAAGATCGCCCCCGCCGTGGCCAAGGCCGCG
ATGGATTCCGGCGTCGCCACCCGTCCGATCCAGGACTTCGACGCCTACGCCGACCAGTTGGCCCAGTTCGTCTACAAGAC
CAACCTGTTCATGAAGCCGGTGTTCGCCCAGGCCAAGAAGGATCCGAAGCGCGTGGTGATGACCGAGGGCGAGGACGAGC
GCGTGCTGCACGCCACCCAGGAGATCGTCACCCAGGGCCTGGCCAAGCCGATCCTGGTGGGCCGCCCCAGCGTGATCGAG
AAGCGCATCGAGAAGCTGGGCCTGAAGATGCGTCCGGGCGTGGACTTCGAACTGGTCAACAATGAATCCGACCCGCGCTT
CAACGACTACTGGCAGGACTACTACCAGCTGATGAAGCGCAAGGGCGTGTCGCAGGAGCAGGCCCGCCGCCGCGTGATCG
GCAACACCACGCTGATCGGCGCGATGATGGTGCGCCGCGGCGACGCCGACGCGCTGATCTGCGGCACCTACGGCCCGTAC
CGCCAGCACTTCGACATCCTGGAGAACGTGCTGGGCTACGCCAACAAGGACAAGGTGGCCGGCGCGATGAACGCGCTGAT
CCTGCCGACCGGCAACATCTTCATCACCGACACCTACGTCAACGCCGAGCCCTCCGCCGAGCAGCTGGCCGCCATCACCA
AGATGGCCGCGCAAAGCATCCAGCGCTTCGGCATCCAGCCGAAGGCCGCGCTGCTGTCCAACTCCAGCTTCGGCGCACTT
AACACCGCGTCGGCGCAGAAGATGCGCGCCGCCTTCGACCAGATCCGCGCCGCCCAGCCGGAACTGGAAATCGACGGCGA
GATGCAGGGCGACGCCGCGCTGGTGGAAAGCATCCGCCAGCAGGCGATGCCGGACTCCACGCTGAAGGGCTCGGCCAATC
TGCTGATCATGCCGAACGTGGAAGCCGCCAACATCAGCTACAACCTGCTGCGCGTGTCGGCGTCCGACGGCGTGACCATC
GGCCCGATCCTGATGGGCATGGCCAAGCCGGCGCACATCCTGACCCCGATCTCGTCGGTGCGCCGCATCGTCAACATGGT
GGCGCTGGCCTCGGTCGACGCCCAGGTCGCCGCGCAACAGGGCTGA

Upstream 100 bases:

>100_bases
GTCCGACACGGCATAGCCCAGGCCTGGCATAAGCCTTATAATGCGAGGCTGATTCTCCCTTACCGACATAAAAGAACATT
TCTCAATCAGGAGCCTGCGA

Downstream 100 bases:

>100_bases
TCCGGCGCCTCAGGCGTCTTATACTGAACGGGCAAGCGTCGCTTGCCCGTTTTTTCATTGCAGGAAACGTCGATGAGCCA
AGACAACCATAACGACCCGT

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 761; Mature: 761

Protein sequence:

>761_residues
MDEQLRQSALEFHQFPTPGKIQVAPTKPLTTQRDLALAYSPGVTAPCDAIVEDPLNAYKYTARGNLVAVISNGTAVLGLG
NIGALASKPVMEGKGVLFKKFAGIDVFDIEVNELDPDKLVDIICSLEPTFGGINLEDIKAPECFYIEKKCRERMGIPVFH
DDQHGTAIVAAAAVLNGLRLVKKEIGEVRVVASGAGAAAIACLDLLVALGVKRENITICDSKGVVFVGRDEKLDESKLRY
AIQDNGWRKLADAMAGADIFMGLSGPRLVSQDMVKSMARDPLILAMANPEPEILPPLVKEVRPDAIIGTGRSDFPNQVNN
VLCFPFIFRGALDVGATTINEEMKLATVRAIADLAMAEQNDVVASAYGDQELSFGPEYVIPKPFDPRLIVKIAPAVAKAA
MDSGVATRPIQDFDAYADQLAQFVYKTNLFMKPVFAQAKKDPKRVVMTEGEDERVLHATQEIVTQGLAKPILVGRPSVIE
KRIEKLGLKMRPGVDFELVNNESDPRFNDYWQDYYQLMKRKGVSQEQARRRVIGNTTLIGAMMVRRGDADALICGTYGPY
RQHFDILENVLGYANKDKVAGAMNALILPTGNIFITDTYVNAEPSAEQLAAITKMAAQSIQRFGIQPKAALLSNSSFGAL
NTASAQKMRAAFDQIRAAQPELEIDGEMQGDAALVESIRQQAMPDSTLKGSANLLIMPNVEAANISYNLLRVSASDGVTI
GPILMGMAKPAHILTPISSVRRIVNMVALASVDAQVAAQQG

Sequences:

>Translated_761_residues
MDEQLRQSALEFHQFPTPGKIQVAPTKPLTTQRDLALAYSPGVTAPCDAIVEDPLNAYKYTARGNLVAVISNGTAVLGLG
NIGALASKPVMEGKGVLFKKFAGIDVFDIEVNELDPDKLVDIICSLEPTFGGINLEDIKAPECFYIEKKCRERMGIPVFH
DDQHGTAIVAAAAVLNGLRLVKKEIGEVRVVASGAGAAAIACLDLLVALGVKRENITICDSKGVVFVGRDEKLDESKLRY
AIQDNGWRKLADAMAGADIFMGLSGPRLVSQDMVKSMARDPLILAMANPEPEILPPLVKEVRPDAIIGTGRSDFPNQVNN
VLCFPFIFRGALDVGATTINEEMKLATVRAIADLAMAEQNDVVASAYGDQELSFGPEYVIPKPFDPRLIVKIAPAVAKAA
MDSGVATRPIQDFDAYADQLAQFVYKTNLFMKPVFAQAKKDPKRVVMTEGEDERVLHATQEIVTQGLAKPILVGRPSVIE
KRIEKLGLKMRPGVDFELVNNESDPRFNDYWQDYYQLMKRKGVSQEQARRRVIGNTTLIGAMMVRRGDADALICGTYGPY
RQHFDILENVLGYANKDKVAGAMNALILPTGNIFITDTYVNAEPSAEQLAAITKMAAQSIQRFGIQPKAALLSNSSFGAL
NTASAQKMRAAFDQIRAAQPELEIDGEMQGDAALVESIRQQAMPDSTLKGSANLLIMPNVEAANISYNLLRVSASDGVTI
GPILMGMAKPAHILTPISSVRRIVNMVALASVDAQVAAQQG
>Mature_761_residues
MDEQLRQSALEFHQFPTPGKIQVAPTKPLTTQRDLALAYSPGVTAPCDAIVEDPLNAYKYTARGNLVAVISNGTAVLGLG
NIGALASKPVMEGKGVLFKKFAGIDVFDIEVNELDPDKLVDIICSLEPTFGGINLEDIKAPECFYIEKKCRERMGIPVFH
DDQHGTAIVAAAAVLNGLRLVKKEIGEVRVVASGAGAAAIACLDLLVALGVKRENITICDSKGVVFVGRDEKLDESKLRY
AIQDNGWRKLADAMAGADIFMGLSGPRLVSQDMVKSMARDPLILAMANPEPEILPPLVKEVRPDAIIGTGRSDFPNQVNN
VLCFPFIFRGALDVGATTINEEMKLATVRAIADLAMAEQNDVVASAYGDQELSFGPEYVIPKPFDPRLIVKIAPAVAKAA
MDSGVATRPIQDFDAYADQLAQFVYKTNLFMKPVFAQAKKDPKRVVMTEGEDERVLHATQEIVTQGLAKPILVGRPSVIE
KRIEKLGLKMRPGVDFELVNNESDPRFNDYWQDYYQLMKRKGVSQEQARRRVIGNTTLIGAMMVRRGDADALICGTYGPY
RQHFDILENVLGYANKDKVAGAMNALILPTGNIFITDTYVNAEPSAEQLAAITKMAAQSIQRFGIQPKAALLSNSSFGAL
NTASAQKMRAAFDQIRAAQPELEIDGEMQGDAALVESIRQQAMPDSTLKGSANLLIMPNVEAANISYNLLRVSASDGVTI
GPILMGMAKPAHILTPISSVRRIVNMVALASVDAQVAAQQG

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=348, Percent_Identity=26.4367816091954, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI62420882, Length=354, Percent_Identity=26.271186440678, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI62420880, Length=354, Percent_Identity=26.271186440678, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI239049447, Length=354, Percent_Identity=26.271186440678, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788806, Length=755, Percent_Identity=73.6423841059603, Blast_Score=1190, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=329, Percent_Identity=29.7872340425532, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1788800, Length=326, Percent_Identity=29.7546012269939, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6322823, Length=240, Percent_Identity=28.3333333333333, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356279, Length=474, Percent_Identity=24.0506329113924, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362672, Length=474, Percent_Identity=24.0506329113924, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281362674, Length=474, Percent_Identity=24.0506329113924, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 82369; Mature: 82369

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEQLRQSALEFHQFPTPGKIQVAPTKPLTTQRDLALAYSPGVTAPCDAIVEDPLNAYKY
CCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHCCHHHEEE
TARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGIDVFDIEVNELDPDKLV
ECCCCEEEEEECCEEEEEECCCCCHHCCCCCCCCCCEEEHCCCCEEEEEEECCCCHHHHH
DIICSLEPTFGGINLEDIKAPECFYIEKKCRERMGIPVFHDDQHGTAIVAAAAVLNGLRL
HHHHCCCCCCCCCCHHHCCCCCEEEEHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
VKKEIGEVRVVASGAGAAAIACLDLLVALGVKRENITICDSKGVVFVGRDEKLDESKLRY
HHHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCHHHHEE
AIQDNGWRKLADAMAGADIFMGLSGPRLVSQDMVKSMARDPLILAMANPEPEILPPLVKE
EECCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
VRPDAIIGTGRSDFPNQVNNVLCFPFIFRGALDVGATTINEEMKLATVRAIADLAMAEQN
CCCCCEEECCCCCCCHHHHCEEEHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHCCC
DVVASAYGDQELSFGPEYVIPKPFDPRLIVKIAPAVAKAAMDSGVATRPIQDFDAYADQL
CEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHH
AQFVYKTNLFMKPVFAQAKKDPKRVVMTEGEDERVLHATQEIVTQGLAKPILVGRPSVIE
HHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCHHHH
KRIEKLGLKMRPGVDFELVNNESDPRFNDYWQDYYQLMKRKGVSQEQARRRVIGNTTLIG
HHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
AMMVRRGDADALICGTYGPYRQHFDILENVLGYANKDKVAGAMNALILPTGNIFITDTYV
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHCEEEEECCCEEEEEEEE
NAEPSAEQLAAITKMAAQSIQRFGIQPKAALLSNSSFGALNTASAQKMRAAFDQIRAAQP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
ELEIDGEMQGDAALVESIRQQAMPDSTLKGSANLLIMPNVEAANISYNLLRVSASDGVTI
CCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECCCCCEE
GPILMGMAKPAHILTPISSVRRIVNMVALASVDAQVAAQQG
HHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MDEQLRQSALEFHQFPTPGKIQVAPTKPLTTQRDLALAYSPGVTAPCDAIVEDPLNAYKY
CCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHCCHHHEEE
TARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGIDVFDIEVNELDPDKLV
ECCCCEEEEEECCEEEEEECCCCCHHCCCCCCCCCCEEEHCCCCEEEEEEECCCCHHHHH
DIICSLEPTFGGINLEDIKAPECFYIEKKCRERMGIPVFHDDQHGTAIVAAAAVLNGLRL
HHHHCCCCCCCCCCHHHCCCCCEEEEHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
VKKEIGEVRVVASGAGAAAIACLDLLVALGVKRENITICDSKGVVFVGRDEKLDESKLRY
HHHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCHHHHEE
AIQDNGWRKLADAMAGADIFMGLSGPRLVSQDMVKSMARDPLILAMANPEPEILPPLVKE
EECCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
VRPDAIIGTGRSDFPNQVNNVLCFPFIFRGALDVGATTINEEMKLATVRAIADLAMAEQN
CCCCCEEECCCCCCCHHHHCEEEHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHCCC
DVVASAYGDQELSFGPEYVIPKPFDPRLIVKIAPAVAKAAMDSGVATRPIQDFDAYADQL
CEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHH
AQFVYKTNLFMKPVFAQAKKDPKRVVMTEGEDERVLHATQEIVTQGLAKPILVGRPSVIE
HHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCHHHH
KRIEKLGLKMRPGVDFELVNNESDPRFNDYWQDYYQLMKRKGVSQEQARRRVIGNTTLIG
HHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
AMMVRRGDADALICGTYGPYRQHFDILENVLGYANKDKVAGAMNALILPTGNIFITDTYV
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHCEEEEECCCEEEEEEEE
NAEPSAEQLAAITKMAAQSIQRFGIQPKAALLSNSSFGALNTASAQKMRAAFDQIRAAQP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
ELEIDGEMQGDAALVESIRQQAMPDSTLKGSANLLIMPNVEAANISYNLLRVSASDGVTI
CCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECCCCCEE
GPILMGMAKPAHILTPISSVRRIVNMVALASVDAQVAAQQG
HHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]