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The map label for this gene is mmr [H]

Identifier: 34496174

GI number: 34496174

Start: 745904

End: 747289

Strand: Reverse

Name: mmr [H]

Synonym: CV_0719

Alternate gene names: 34496174

Gene position: 747289-745904 (Counterclockwise)

Preceding gene: 34496176

Following gene: 34496173

Centisome position: 15.73

GC content: 73.59

Gene sequence:

>1386_bases
ATGCCTCACGCCCAATCGGTCCGGACCGGACTGCCCACCCTGCTGGCGCTCAGCGCCGGCATGTTCATGGCGGTGCTCGA
CACCAATATCGTCAATCTGGCGCTGCCGGCCTTGCTGCGCAGCCTGGACGCCAGCCTCAGCCAACTGACCTGGATCGTGG
ACGCTTACGCGCTGGGCTTCGCCGGCCTGATGCTCAGCGCCGGCTGGCTGGCCGACCGCTTCGGCCCCCGGCGGATGCTG
GCCTGGGGGCTGGCCCTGTTCGTGCTGGCCTCGCTGCTGTGCGGGCTGGCGCCCGGCGCGAGGACGCTGATCCTCTTTCG
CTTGCTGCAGGGCATGGCGGCGGCCTTGTTCATTCCCAGCTCGCTCGGCCTGTTGCGCCACGCCTTTCCCCATCCGGCCG
AGCGCGCGCGCGCCGTCGGCCTGTACGGCGGCCTGTCCGCCTCGGCCGCCGCGGCCGGCCCGGTGCTGGGCGGCCTGCTG
CTGGCGCCGCTGGGCTGGCGCGGCGCCTTCCTGATCAACGTGCCAATCGGTCTGGCCGCGCTGCTGGCCATCCCCTTCGT
GGTGCCGCATCGCCAAGCGATGCCGACCCGCGGCGCCGACCTGCCCGGCCAGGCGGCCGGCATGCTGACGCTGCTGCTGG
CCAGCTACGCGCTGATCGAAGGCCCGCGCCTGGGCGCGCCCGTCCTGTTCGCCCTGCTGCTGGCGGTCGCCGGCTGCGGC
CGCCTGTTCTGGCAGCTGGAAAACGCCAACCCGCAGGCGATGGTGCCGCCCGAGTTGTTCCGCAACCCGGTTTTCGCCGC
CGCCAACTGGGTGGGCTTCGCCATCGGCGTCGCCTATTTCGGCACCCTGCTGCTGCTCAGCCTCTACCTGCAGCAAGGCC
TGGGCCTGGACGCGCTGCATGCCGGCCTGGCCATGCTGCCGCTGGCCGGATGCCTGATCATCGGCAACGTCGCCGCCGGC
CGCCTGCTGCCGCGGTTGGGCGCCCGCCGCCAGATCCGCGGCGGCCTGCTGCTCTCTGCCGCTGGCTATCTGCTGCTGGC
CCTCGCCGACGGCCATGCGCTGGCCTGGTCGCTGCTGGCGATGCTGCCGCTGGCGCTGGGCACCGCGCTCGCCATCGCGC
CGATGACCGCCACCGTGCTGGAAAGCGCGCCGCCGCATCTGTCCGGCACCGCGTCGGCCGTGCTGAACGCCGTCCGCCAA
ACCGGCGCCCTGGTCGGCGCCGCAGGCGCGGCTCTCGCCGTCGGCTATGGCGCCGACCTCCGCCATGCCTTGGCCATCGG
ACTCTCCGTCGCCGCGCTGGCCTCGCTCAGCGGCGCGCTCATCGCAGGCTGGATACCGCGGGCGGGCCAGCAGGCTGCGC
CGGAACCGGCGCCGGCCGCGCGCTGA

Upstream 100 bases:

>100_bases
TTAAAATGCGAAATTGCATCACCATTTATCATATTTGTCTATTTTTTCGCACCATCATCTCGCCTAGCATGGCTGCGTTT
TCCACCTGGGGACGCCATTC

Downstream 100 bases:

>100_bases
CGACGGGCCGGGGCAGCGGCATGGCCTCGCAGGGCGGGCGATAGCGGGCATCCCTGGCCAAAGGGCTAGCCATGGGACCC
CGCGCCGGTCTGCCATACTC

Product: methylenomycin A resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: MMR peptide [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MPHAQSVRTGLPTLLALSAGMFMAVLDTNIVNLALPALLRSLDASLSQLTWIVDAYALGFAGLMLSAGWLADRFGPRRML
AWGLALFVLASLLCGLAPGARTLILFRLLQGMAAALFIPSSLGLLRHAFPHPAERARAVGLYGGLSASAAAAGPVLGGLL
LAPLGWRGAFLINVPIGLAALLAIPFVVPHRQAMPTRGADLPGQAAGMLTLLLASYALIEGPRLGAPVLFALLLAVAGCG
RLFWQLENANPQAMVPPELFRNPVFAAANWVGFAIGVAYFGTLLLLSLYLQQGLGLDALHAGLAMLPLAGCLIIGNVAAG
RLLPRLGARRQIRGGLLLSAAGYLLLALADGHALAWSLLAMLPLALGTALAIAPMTATVLESAPPHLSGTASAVLNAVRQ
TGALVGAAGAALAVGYGADLRHALAIGLSVAALASLSGALIAGWIPRAGQQAAPEPAPAAR

Sequences:

>Translated_461_residues
MPHAQSVRTGLPTLLALSAGMFMAVLDTNIVNLALPALLRSLDASLSQLTWIVDAYALGFAGLMLSAGWLADRFGPRRML
AWGLALFVLASLLCGLAPGARTLILFRLLQGMAAALFIPSSLGLLRHAFPHPAERARAVGLYGGLSASAAAAGPVLGGLL
LAPLGWRGAFLINVPIGLAALLAIPFVVPHRQAMPTRGADLPGQAAGMLTLLLASYALIEGPRLGAPVLFALLLAVAGCG
RLFWQLENANPQAMVPPELFRNPVFAAANWVGFAIGVAYFGTLLLLSLYLQQGLGLDALHAGLAMLPLAGCLIIGNVAAG
RLLPRLGARRQIRGGLLLSAAGYLLLALADGHALAWSLLAMLPLALGTALAIAPMTATVLESAPPHLSGTASAVLNAVRQ
TGALVGAAGAALAVGYGADLRHALAIGLSVAALASLSGALIAGWIPRAGQQAAPEPAPAAR
>Mature_460_residues
PHAQSVRTGLPTLLALSAGMFMAVLDTNIVNLALPALLRSLDASLSQLTWIVDAYALGFAGLMLSAGWLADRFGPRRMLA
WGLALFVLASLLCGLAPGARTLILFRLLQGMAAALFIPSSLGLLRHAFPHPAERARAVGLYGGLSASAAAAGPVLGGLLL
APLGWRGAFLINVPIGLAALLAIPFVVPHRQAMPTRGADLPGQAAGMLTLLLASYALIEGPRLGAPVLFALLLAVAGCGR
LFWQLENANPQAMVPPELFRNPVFAAANWVGFAIGVAYFGTLLLLSLYLQQGLGLDALHAGLAMLPLAGCLIIGNVAAGR
LLPRLGARRQIRGGLLLSAAGYLLLALADGHALAWSLLAMLPLALGTALAIAPMTATVLESAPPHLSGTASAVLNAVRQT
GALVGAAGAALAVGYGADLRHALAIGLSVAALASLSGALIAGWIPRAGQQAAPEPAPAAR

Specific function: Resistance to the epoxide antibiotic methylenomycin A; probably by mediating its efflux [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=423, Percent_Identity=29.5508274231678, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI87081983, Length=428, Percent_Identity=30.8411214953271, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1790195, Length=339, Percent_Identity=34.5132743362832, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788710, Length=425, Percent_Identity=25.6470588235294, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=29.683698296837, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790146, Length=170, Percent_Identity=30, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=386, Percent_Identity=24.6113989637306, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47001; Mature: 46869

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHAQSVRTGLPTLLALSAGMFMAVLDTNIVNLALPALLRSLDASLSQLTWIVDAYALGF
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLMLSAGWLADRFGPRRMLAWGLALFVLASLLCGLAPGARTLILFRLLQGMAAALFIPS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
SLGLLRHAFPHPAERARAVGLYGGLSASAAAAGPVLGGLLLAPLGWRGAFLINVPIGLAA
HHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
LLAIPFVVPHRQAMPTRGADLPGQAAGMLTLLLASYALIEGPRLGAPVLFALLLAVAGCG
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RLFWQLENANPQAMVPPELFRNPVFAAANWVGFAIGVAYFGTLLLLSLYLQQGLGLDALH
HHHEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AGLAMLPLAGCLIIGNVAAGRLLPRLGARRQIRGGLLLSAAGYLLLALADGHALAWSLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHH
MLPLALGTALAIAPMTATVLESAPPHLSGTASAVLNAVRQTGALVGAAGAALAVGYGADL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHH
RHALAIGLSVAALASLSGALIAGWIPRAGQQAAPEPAPAAR
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PHAQSVRTGLPTLLALSAGMFMAVLDTNIVNLALPALLRSLDASLSQLTWIVDAYALGF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLMLSAGWLADRFGPRRMLAWGLALFVLASLLCGLAPGARTLILFRLLQGMAAALFIPS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
SLGLLRHAFPHPAERARAVGLYGGLSASAAAAGPVLGGLLLAPLGWRGAFLINVPIGLAA
HHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
LLAIPFVVPHRQAMPTRGADLPGQAAGMLTLLLASYALIEGPRLGAPVLFALLLAVAGCG
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RLFWQLENANPQAMVPPELFRNPVFAAANWVGFAIGVAYFGTLLLLSLYLQQGLGLDALH
HHHEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AGLAMLPLAGCLIIGNVAAGRLLPRLGARRQIRGGLLLSAAGYLLLALADGHALAWSLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHH
MLPLALGTALAIAPMTATVLESAPPHLSGTASAVLNAVRQTGALVGAAGAALAVGYGADL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHH
RHALAIGLSVAALASLSGALIAGWIPRAGQQAAPEPAPAAR
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2828187; 12000953 [H]