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The map label for this gene is gcvA [H]

Identifier: 34496151

GI number: 34496151

Start: 722226

End: 723104

Strand: Reverse

Name: gcvA [H]

Synonym: CV_0696

Alternate gene names: 34496151

Gene position: 723104-722226 (Counterclockwise)

Preceding gene: 34496153

Following gene: 34496150

Centisome position: 15.22

GC content: 72.92

Gene sequence:

>879_bases
ATGAAACGAGATCTCCCGCCGCTGAACGCCTTGCGCGCGTTCGAGGCCGCCGCCGTCCGGCAGAGCTTCAGCGAGGCCGC
CGAGCTGCTGCACGTCACCCACGGCGCGGTCAGCCGGCAGATCCGCCTGCTGGAGGCGTGGCTGGGCACGCCGCTGTTCG
AGCGCCATCACCGCCAGGTGCGGCTCACCGACGCCGGCCGCGGCTACCTCGCCGACGTCCGCGCCGCGCTGGAGCGGATA
GAAACCGCCAGCGCCAGGCTGCGGCAGGGTCCGCTGCCGCTGCGGGTCAACGCGCCGGCCACCTTCACGCTGCGCTGGCT
GATCCCCAGGCTGTCGCGCTTCCAGCTGGCGCATCCGCACGTGGAAGTGAAGCTGACCACCTCCAGCGAGCCCGTCGCCC
AGCTGGCCGACAGCTTCGAGGTGGCGATACGCGGCGGCCCGCAGGCGCTGCCGGGCTTCATCGCCCATGAATTCCTGCAG
GAGAGCCGGCTGCCGGTGTGCAGCCCCGGCCTGCTCGAACGCCTGCCGCTCGCCTCCCCGGCCGATCTCGTCCGCCACAC
CCTGCTGCACACCGCCGGCAGCCCCGAGTTCTGGCCGCAGTGGCTGGACGCCGCCGGCGTGCCGGACCTGCAGCCGGCCG
CCAGCCTGACGCTGGAGCATTTCTACCTGACGCTGCAGGCGGCGCTGGACGGGCTGGGCGTGGCCTTGGGCCCCAGCCGG
CTGATCGCCGACGACCTGGCCGCCGGCCGCCTGGTGCAGCCGTTCGCCGGCCCGCGGCTGGAGGACTGGCGCTATTTCGC
CTACGTGCCGGAAACGCGGGACGGGCATCCCGCGGTCGCCGCATTCTGCCGTTGGCTGGCGCAGGCCGATCATGGATAA

Upstream 100 bases:

>100_bases
GAAGTGAAAATTGATGCTGTTGCGACAACACCTTACGCAACTTGCCCGCAGGTCGACAAACGACTTATTCTGGCTTTTCA
TGTCAGGAAAACTCACACGA

Downstream 100 bases:

>100_bases
TCCGACATCGCACAAGCACATTGTCATGCTCGACGCGCTCCGCCGGTTCGGCTATATCTAAAAACCGCCGCCGGGCCGCG
CGGGAACTCCGCGCGGCCGG

Product: transcription regulator protein

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKRDLPPLNALRAFEAAAVRQSFSEAAELLHVTHGAVSRQIRLLEAWLGTPLFERHHRQVRLTDAGRGYLADVRAALERI
ETASARLRQGPLPLRVNAPATFTLRWLIPRLSRFQLAHPHVEVKLTTSSEPVAQLADSFEVAIRGGPQALPGFIAHEFLQ
ESRLPVCSPGLLERLPLASPADLVRHTLLHTAGSPEFWPQWLDAAGVPDLQPAASLTLEHFYLTLQAALDGLGVALGPSR
LIADDLAAGRLVQPFAGPRLEDWRYFAYVPETRDGHPAVAAFCRWLAQADHG

Sequences:

>Translated_292_residues
MKRDLPPLNALRAFEAAAVRQSFSEAAELLHVTHGAVSRQIRLLEAWLGTPLFERHHRQVRLTDAGRGYLADVRAALERI
ETASARLRQGPLPLRVNAPATFTLRWLIPRLSRFQLAHPHVEVKLTTSSEPVAQLADSFEVAIRGGPQALPGFIAHEFLQ
ESRLPVCSPGLLERLPLASPADLVRHTLLHTAGSPEFWPQWLDAAGVPDLQPAASLTLEHFYLTLQAALDGLGVALGPSR
LIADDLAAGRLVQPFAGPRLEDWRYFAYVPETRDGHPAVAAFCRWLAQADHG
>Mature_292_residues
MKRDLPPLNALRAFEAAAVRQSFSEAAELLHVTHGAVSRQIRLLEAWLGTPLFERHHRQVRLTDAGRGYLADVRAALERI
ETASARLRQGPLPLRVNAPATFTLRWLIPRLSRFQLAHPHVEVKLTTSSEPVAQLADSFEVAIRGGPQALPGFIAHEFLQ
ESRLPVCSPGLLERLPLASPADLVRHTLLHTAGSPEFWPQWLDAAGVPDLQPAASLTLEHFYLTLQAALDGLGVALGPSR
LIADDLAAGRLVQPFAGPRLEDWRYFAYVPETRDGHPAVAAFCRWLAQADHG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=37.0242214532872, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=30.9278350515464, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=31.8181818181818, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=29.0657439446367, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786401, Length=244, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790208, Length=171, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081978, Length=266, Percent_Identity=28.1954887218045, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=161, Percent_Identity=34.1614906832298, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787128, Length=260, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788887, Length=283, Percent_Identity=28.9752650176678, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787949, Length=80, Percent_Identity=41.25, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787589, Length=298, Percent_Identity=24.496644295302, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789440, Length=239, Percent_Identity=25.1046025104602, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32147; Mature: 32147

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDLPPLNALRAFEAAAVRQSFSEAAELLHVTHGAVSRQIRLLEAWLGTPLFERHHRQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEE
RLTDAGRGYLADVRAALERIETASARLRQGPLPLRVNAPATFTLRWLIPRLSRFQLAHPH
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEHHHHHHHHHHHHEECCCC
VEVKLTTSSEPVAQLADSFEVAIRGGPQALPGFIAHEFLQESRLPVCSPGLLERLPLASP
EEEEEECCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCH
ADLVRHTLLHTAGSPEFWPQWLDAAGVPDLQPAASLTLEHFYLTLQAALDGLGVALGPSR
HHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LIADDLAAGRLVQPFAGPRLEDWRYFAYVPETRDGHPAVAAFCRWLAQADHG
HHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRDLPPLNALRAFEAAAVRQSFSEAAELLHVTHGAVSRQIRLLEAWLGTPLFERHHRQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEE
RLTDAGRGYLADVRAALERIETASARLRQGPLPLRVNAPATFTLRWLIPRLSRFQLAHPH
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEHHHHHHHHHHHHEECCCC
VEVKLTTSSEPVAQLADSFEVAIRGGPQALPGFIAHEFLQESRLPVCSPGLLERLPLASP
EEEEEECCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCH
ADLVRHTLLHTAGSPEFWPQWLDAAGVPDLQPAASLTLEHFYLTLQAALDGLGVALGPSR
HHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LIADDLAAGRLVQPFAGPRLEDWRYFAYVPETRDGHPAVAAFCRWLAQADHG
HHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]