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The map label for this gene is pilR [H]

Identifier: 34496143

GI number: 34496143

Start: 712430

End: 713764

Strand: Reverse

Name: pilR [H]

Synonym: CV_0688

Alternate gene names: 34496143

Gene position: 713764-712430 (Counterclockwise)

Preceding gene: 34496144

Following gene: 34496137

Centisome position: 15.02

GC content: 62.02

Gene sequence:

>1335_bases
ATGCAAGCGAGCAAAACGCTGCTGGCGCTAGGGTCGGATGCATTGCTTCCCGTGCTGCACGATTTGCATCAGCAGCACTG
GAAGATCATTCACATCCACGATGTGCAGCAGCCCAAACTCCGGCAGGCTGGGCCATTCAGCGCGGGCCTGCTCTTGCTGG
ACAACAAGCTGCTGCGCAAGGCGGAGGCGCTGCAGCAGCTTGGCGGCCAGCTTGACCTGGAGTGGGTGTTGCTAACCGAA
CGCCAACAACTGGAGCACGCAGCCGTGCGTAGCTTCATCAATGACGGCTGCTATGACTACCATTGTTTGCCGGTCGAGAG
CGCCAGGCTGGCGGTCACGCTGGGGCGGCTGCATGGCAAGACCATGCTGCGGAGGCATCTGCAGCAGGAGGAGATCCCGG
AGGAGCAAAAAAAACTGGTTGGCGAGCATGACTGCATGCGGCGTTTGCGTCAGGAAATCCGCCGTCTGGCGCGCGTGAAT
GTCACCGTCGTCATCACCGGGGAGTCCGGTTGCGGCAAGGAGCTGGTGGCTCAGCTGTTGCATCAGTACTCCGACCGGGC
CGATGGCCCTTTCCAGACAGTCAACTGCGGCGCGCTGCCGGTCAGCCTGGTGCAGTCGGAATTATTCGGCCACGAGCGAG
GCGCCTTTACCGGCGCTTCATCCGGTCGTATCGGCCGCATCGAGGCTGCCCACAATGGCACCCTGTTTCTGGATGAAATC
GGAGACATGGCCATTGAAGCGCAGGTGAGCCTGCTGCGCTTCCTGCAGGAAGGCACCATAGACCGGCTGGGCGACCATCG
CAGCCGCAGCATCGACACCCGTGTCTTGTCGGCAACCCACGTCGACCTGCCCGCCGCCATCAGCAACGGTCGTTTCCGTC
AGGACTTGTATTACCGCCTGTGCGTCTGCCAGCTGCACCTGCCGCCCTTGCGCGAGCGGCGTTCCGATATTCCCCTGCTG
GCCAAGCACCTGCTATTCCAGAATGCCCAGAGGCTGGGTTTGCCGCACAAACGGCTGTCGTACGAGGCAATGGCCATCAT
GTTGCAACATGACTGGCCCGGTAATATCCGGGAGATGTCCAACCGCATCAGCCAGGGCTTGGCCATGGCCGGCGGGCGGG
TCATCCGGCCGCAGGACATGGGGCTGGGCGGCAATATGGCCATCCCGCTGCATGATGGCCTGGAAGCCGTCCGGGAAAAG
GCCGAGCGCGAGGCGGTGGAGATGGCGCTGGAGCTGCATGGCCATAACAGCAGCAAGGCGGCGCGCATGCTGTGCATCTC
GCGCGCCACGCTGTACCGGCTGTTGAAGAAATATTCGATGCAAATCATCAACTAG

Upstream 100 bases:

>100_bases
GGCTCTCTATCATTCCCAGCCTGTCGCTGGGCATGACGCCGGACAGCCCGAACTATGCATTTTCGGTGAAGTTCCCTTAT
CGATTCTAACGGAGTCGGCA

Downstream 100 bases:

>100_bases
CGACAGCACAACAGGTGACCACCGGGCCACGGCGCACGGCGTCGGCAAGAAACGACATGGCGGGCGATGTTCAGCGCAGC
CCGATGCCGGCCATCATGGC

Product: transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MQASKTLLALGSDALLPVLHDLHQQHWKIIHIHDVQQPKLRQAGPFSAGLLLLDNKLLRKAEALQQLGGQLDLEWVLLTE
RQQLEHAAVRSFINDGCYDYHCLPVESARLAVTLGRLHGKTMLRRHLQQEEIPEEQKKLVGEHDCMRRLRQEIRRLARVN
VTVVITGESGCGKELVAQLLHQYSDRADGPFQTVNCGALPVSLVQSELFGHERGAFTGASSGRIGRIEAAHNGTLFLDEI
GDMAIEAQVSLLRFLQEGTIDRLGDHRSRSIDTRVLSATHVDLPAAISNGRFRQDLYYRLCVCQLHLPPLRERRSDIPLL
AKHLLFQNAQRLGLPHKRLSYEAMAIMLQHDWPGNIREMSNRISQGLAMAGGRVIRPQDMGLGGNMAIPLHDGLEAVREK
AEREAVEMALELHGHNSSKAARMLCISRATLYRLLKKYSMQIIN

Sequences:

>Translated_444_residues
MQASKTLLALGSDALLPVLHDLHQQHWKIIHIHDVQQPKLRQAGPFSAGLLLLDNKLLRKAEALQQLGGQLDLEWVLLTE
RQQLEHAAVRSFINDGCYDYHCLPVESARLAVTLGRLHGKTMLRRHLQQEEIPEEQKKLVGEHDCMRRLRQEIRRLARVN
VTVVITGESGCGKELVAQLLHQYSDRADGPFQTVNCGALPVSLVQSELFGHERGAFTGASSGRIGRIEAAHNGTLFLDEI
GDMAIEAQVSLLRFLQEGTIDRLGDHRSRSIDTRVLSATHVDLPAAISNGRFRQDLYYRLCVCQLHLPPLRERRSDIPLL
AKHLLFQNAQRLGLPHKRLSYEAMAIMLQHDWPGNIREMSNRISQGLAMAGGRVIRPQDMGLGGNMAIPLHDGLEAVREK
AEREAVEMALELHGHNSSKAARMLCISRATLYRLLKKYSMQIIN
>Mature_444_residues
MQASKTLLALGSDALLPVLHDLHQQHWKIIHIHDVQQPKLRQAGPFSAGLLLLDNKLLRKAEALQQLGGQLDLEWVLLTE
RQQLEHAAVRSFINDGCYDYHCLPVESARLAVTLGRLHGKTMLRRHLQQEEIPEEQKKLVGEHDCMRRLRQEIRRLARVN
VTVVITGESGCGKELVAQLLHQYSDRADGPFQTVNCGALPVSLVQSELFGHERGAFTGASSGRIGRIEAAHNGTLFLDEI
GDMAIEAQVSLLRFLQEGTIDRLGDHRSRSIDTRVLSATHVDLPAAISNGRFRQDLYYRLCVCQLHLPPLRERRSDIPLL
AKHLLFQNAQRLGLPHKRLSYEAMAIMLQHDWPGNIREMSNRISQGLAMAGGRVIRPQDMGLGGNMAIPLHDGLEAVREK
AEREAVEMALELHGHNSSKAARMLCISRATLYRLLKKYSMQIIN

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=351, Percent_Identity=42.4501424501425, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1788905, Length=308, Percent_Identity=45.1298701298701, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790299, Length=393, Percent_Identity=36.6412213740458, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788550, Length=383, Percent_Identity=37.8590078328982, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI87082152, Length=321, Percent_Identity=40.809968847352, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1789087, Length=249, Percent_Identity=43.7751004016064, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1789233, Length=328, Percent_Identity=40.5487804878049, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI87082117, Length=386, Percent_Identity=36.2694300518135, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=41.3793103448276, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=35.8024691358025, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787583, Length=310, Percent_Identity=33.8709677419355, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081858, Length=309, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=34.0163934426229, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49910; Mature: 49910

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASKTLLALGSDALLPVLHDLHQQHWKIIHIHDVQQPKLRQAGPFSAGLLLLDNKLLRK
CCCCCCCHHCCCCHHHHHHHHHHHHHCEEEEEECCCCCCHHCCCCCCCCHHHHHHHHHHH
AEALQQLGGQLDLEWVLLTERQQLEHAAVRSFINDGCYDYHCLPVESARLAVTLGRLHGK
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHEEHHHHHHHH
TMLRRHLQQEEIPEEQKKLVGEHDCMRRLRQEIRRLARVNVTVVITGESGCGKELVAQLL
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHH
HQYSDRADGPFQTVNCGALPVSLVQSELFGHERGAFTGASSGRIGRIEAAHNGTLFLDEI
HHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEHH
GDMAIEAQVSLLRFLQEGTIDRLGDHRSRSIDTRVLSATHVDLPAAISNGRFRQDLYYRL
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHH
CVCQLHLPPLRERRSDIPLLAKHLLFQNAQRLGLPHKRLSYEAMAIMLQHDWPGNIREMS
HHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCHHHHH
NRISQGLAMAGGRVIRPQDMGLGGNMAIPLHDGLEAVREKAEREAVEMALELHGHNSSKA
HHHHHHHHHCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ARMLCISRATLYRLLKKYSMQIIN
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQASKTLLALGSDALLPVLHDLHQQHWKIIHIHDVQQPKLRQAGPFSAGLLLLDNKLLRK
CCCCCCCHHCCCCHHHHHHHHHHHHHCEEEEEECCCCCCHHCCCCCCCCHHHHHHHHHHH
AEALQQLGGQLDLEWVLLTERQQLEHAAVRSFINDGCYDYHCLPVESARLAVTLGRLHGK
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHEEHHHHHHHH
TMLRRHLQQEEIPEEQKKLVGEHDCMRRLRQEIRRLARVNVTVVITGESGCGKELVAQLL
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHH
HQYSDRADGPFQTVNCGALPVSLVQSELFGHERGAFTGASSGRIGRIEAAHNGTLFLDEI
HHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEHH
GDMAIEAQVSLLRFLQEGTIDRLGDHRSRSIDTRVLSATHVDLPAAISNGRFRQDLYYRL
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHH
CVCQLHLPPLRERRSDIPLLAKHLLFQNAQRLGLPHKRLSYEAMAIMLQHDWPGNIREMS
HHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCHHHHH
NRISQGLAMAGGRVIRPQDMGLGGNMAIPLHDGLEAVREKAEREAVEMALELHGHNSSKA
HHHHHHHHHCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ARMLCISRATLYRLLKKYSMQIIN
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]