Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 34495888

GI number: 34495888

Start: 450477

End: 451877

Strand: Reverse

Name: oprM [H]

Synonym: CV_0433

Alternate gene names: 34495888

Gene position: 451877-450477 (Counterclockwise)

Preceding gene: 34495889

Following gene: 34495882

Centisome position: 9.51

GC content: 71.38

Gene sequence:

>1401_bases
ATGCTTAAGCGCGCCCTGATTCCCGTCGCCGTCGCGCTCGCCGTGTCGGCCTGCGCGGTGGGGCCGGACTACAGCCGGCC
CAAGGTCGAGCTGCCGGAGACCCAGGCCGGCGCCCGGGCGCCGGCGGTGGACGCCGGCTGGTGGAAACGCTTCGACGACC
CGGTGTTGAACCAGCTGATCGAGGAGGCGGTCAAGAGCAACCTCGACCTGCAAGCCGCCGCCGCCCGCGTCGACCAGGCC
GCCGCCCAGGCCGGCATCGCCCGCGCGGCGCTGCTGCCTCAGCTTAACGCCAACGCCGGCTACCAGCGCGGCCGCAGCTC
CACCGAGCTGGCCACCCCGGGCGCGCCGCTGGTCAGCGATGCCCGCTCGGCCAACCTGACCGCGTCCTGGGAGCTGGACT
TGTGGGGCAAGCTGCGCCGCAGCAATGAAGCCGCGCGCGCCAGCTACCAGGCCAGCCGCTACGACCGCGATGCCGCCCAA
CTGGCGCTGGCGGCGCAAGTGGCGCAGACCTACTTCCAGATGCGGGCGCTGGACGCCCAGCTGGACATCGCCAAACGCAC
GCTGCAAAGCCGCGAAGAGTCTCTGAAGCTGCAGACCAAGCGCTTCCACGGCGGCCTGATCTCCGAGCTGGACCAGCGCC
AGGCCGAGGTGGAGGCCGCATCGGCCCGCGCCGCCGTGCCGCAGTTCGCCCGCTCGCTGGAGCAGACCGAAACCGCGCTC
GGCGTGCTGCTGGGCCACAGCCCCAAGCAACTGGTCGACGGCGGCCTCGCGCGCGGCAAGGACATCAACAGCCTGGCCAG
CCCGCCCGAGGTGCCGGCCGACCTGCCGTCCAGCCTGCTGGAGCGCCGCCCCGACGTCGCCGCCAGCGAGCAGCAGCTGA
TCGCCGCCAACGCCCGCATCGGCGTGGCCAAGGCCGCCTACTTTCCCAGCATCAGCCTGAGCGGCGCGCTGGGATCGCAG
AGCCTGTCGCTGGACAGCCTGTTCACCGGCCCCACCCGCGCCTGGAGCTTCGCCGGCAATCTGGCCGCGCCGATCTTCAA
CTTCGGCGCCACCGGCTATAACGTGGACGCCGCCACCGCCGGCCAGAAGCAGGCGCTGGCCCAGTACCAGAAGACGGTGC
AGTCGGCGTTCAAGGACGCGCTGGATGCGCTGGCCGCCAACGGCGCCGCCCGCGACATCCTGCAGGCGCAGACCACGCAA
TCGACGGCGCTGAACCGCACGCTGAAGCTGGCCGGCCTGCGCTACGACAACGGCTACGCCAGCTACCTGGACGTGCTGGA
CGCGCAGCGCAACAGCTTCCAGGCCGAGCTGAACCTGGTCAACGCCAAGCTGGACAAGCTGAACACCACCATCGGCGTCT
ACAAGGCGCTGGGCGGGGGGTGGGAGGCGGAGAAGCGGTAA

Upstream 100 bases:

>100_bases
TTCATCGCCACCTTCTTCATCCCGCTGTTCTTCATCCTGATCATGAAGCTCGGCAAGCAGAACAAGCCTCAGCAGAACGC
CGAGGCCGGAGGAAACGCAG

Downstream 100 bases:

>100_bases
CGCTTTCCTGCCGCTTGAATGAAAAAACCTGCCTGGAAACAGGCAGGTTTTTTTATAACTAACGACAACAAGTCTTCCGT
CAAACTACCTCATCCAGCTC

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MLKRALIPVAVALAVSACAVGPDYSRPKVELPETQAGARAPAVDAGWWKRFDDPVLNQLIEEAVKSNLDLQAAAARVDQA
AAQAGIARAALLPQLNANAGYQRGRSSTELATPGAPLVSDARSANLTASWELDLWGKLRRSNEAARASYQASRYDRDAAQ
LALAAQVAQTYFQMRALDAQLDIAKRTLQSREESLKLQTKRFHGGLISELDQRQAEVEAASARAAVPQFARSLEQTETAL
GVLLGHSPKQLVDGGLARGKDINSLASPPEVPADLPSSLLERRPDVAASEQQLIAANARIGVAKAAYFPSISLSGALGSQ
SLSLDSLFTGPTRAWSFAGNLAAPIFNFGATGYNVDAATAGQKQALAQYQKTVQSAFKDALDALAANGAARDILQAQTTQ
STALNRTLKLAGLRYDNGYASYLDVLDAQRNSFQAELNLVNAKLDKLNTTIGVYKALGGGWEAEKR

Sequences:

>Translated_466_residues
MLKRALIPVAVALAVSACAVGPDYSRPKVELPETQAGARAPAVDAGWWKRFDDPVLNQLIEEAVKSNLDLQAAAARVDQA
AAQAGIARAALLPQLNANAGYQRGRSSTELATPGAPLVSDARSANLTASWELDLWGKLRRSNEAARASYQASRYDRDAAQ
LALAAQVAQTYFQMRALDAQLDIAKRTLQSREESLKLQTKRFHGGLISELDQRQAEVEAASARAAVPQFARSLEQTETAL
GVLLGHSPKQLVDGGLARGKDINSLASPPEVPADLPSSLLERRPDVAASEQQLIAANARIGVAKAAYFPSISLSGALGSQ
SLSLDSLFTGPTRAWSFAGNLAAPIFNFGATGYNVDAATAGQKQALAQYQKTVQSAFKDALDALAANGAARDILQAQTTQ
STALNRTLKLAGLRYDNGYASYLDVLDAQRNSFQAELNLVNAKLDKLNTTIGVYKALGGGWEAEKR
>Mature_466_residues
MLKRALIPVAVALAVSACAVGPDYSRPKVELPETQAGARAPAVDAGWWKRFDDPVLNQLIEEAVKSNLDLQAAAARVDQA
AAQAGIARAALLPQLNANAGYQRGRSSTELATPGAPLVSDARSANLTASWELDLWGKLRRSNEAARASYQASRYDRDAAQ
LALAAQVAQTYFQMRALDAQLDIAKRTLQSREESLKLQTKRFHGGLISELDQRQAEVEAASARAAVPQFARSLEQTETAL
GVLLGHSPKQLVDGGLARGKDINSLASPPEVPADLPSSLLERRPDVAASEQQLIAANARIGVAKAAYFPSISLSGALGSQ
SLSLDSLFTGPTRAWSFAGNLAAPIFNFGATGYNVDAATAGQKQALAQYQKTVQSAFKDALDALAANGAARDILQAQTTQ
STALNRTLKLAGLRYDNGYASYLDVLDAQRNSFQAELNLVNAKLDKLNTTIGVYKALGGGWEAEKR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=463, Percent_Identity=34.9892008639309, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI2367348, Length=479, Percent_Identity=26.5135699373695, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49553; Mature: 49553

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRALIPVAVALAVSACAVGPDYSRPKVELPETQAGARAPAVDAGWWKRFDDPVLNQLI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
EEAVKSNLDLQAAAARVDQAAAQAGIARAALLPQLNANAGYQRGRSSTELATPGAPLVSD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCHHHC
ARSANLTASWELDLWGKLRRSNEAARASYQASRYDRDAAQLALAAQVAQTYFQMRALDAQ
CCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDIAKRTLQSREESLKLQTKRFHGGLISELDQRQAEVEAASARAAVPQFARSLEQTETAL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLLGHSPKQLVDGGLARGKDINSLASPPEVPADLPSSLLERRPDVAASEQQLIAANARI
HHHHCCCHHHHHCCHHCCCCCCHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHCCCC
GVAKAAYFPSISLSGALGSQSLSLDSLFTGPTRAWSFAGNLAAPIFNFGATGYNVDAATA
CHHHHHHCCCCEECCCCCCCCCCHHHHCCCCCHHHHCCCCHHHHHHHCCCCCCCCCCCCC
GQKQALAQYQKTVQSAFKDALDALAANGAARDILQAQTTQSTALNRTLKLAGLRYDNGYA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
SYLDVLDAQRNSFQAELNLVNAKLDKLNTTIGVYKALGGGWEAEKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLKRALIPVAVALAVSACAVGPDYSRPKVELPETQAGARAPAVDAGWWKRFDDPVLNQLI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
EEAVKSNLDLQAAAARVDQAAAQAGIARAALLPQLNANAGYQRGRSSTELATPGAPLVSD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCHHHC
ARSANLTASWELDLWGKLRRSNEAARASYQASRYDRDAAQLALAAQVAQTYFQMRALDAQ
CCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDIAKRTLQSREESLKLQTKRFHGGLISELDQRQAEVEAASARAAVPQFARSLEQTETAL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLLGHSPKQLVDGGLARGKDINSLASPPEVPADLPSSLLERRPDVAASEQQLIAANARI
HHHHCCCHHHHHCCHHCCCCCCHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHCCCC
GVAKAAYFPSISLSGALGSQSLSLDSLFTGPTRAWSFAGNLAAPIFNFGATGYNVDAATA
CHHHHHHCCCCEECCCCCCCCCCHHHHCCCCCHHHHCCCCHHHHHHHCCCCCCCCCCCCC
GQKQALAQYQKTVQSAFKDALDALAANGAARDILQAQTTQSTALNRTLKLAGLRYDNGYA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
SYLDVLDAQRNSFQAELNLVNAKLDKLNTTIGVYKALGGGWEAEKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]