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The map label for this gene is rbsB [H]

Identifier: 34495832

GI number: 34495832

Start: 392231

End: 393172

Strand: Reverse

Name: rbsB [H]

Synonym: CV_0377

Alternate gene names: 34495832

Gene position: 393172-392231 (Counterclockwise)

Preceding gene: 34495833

Following gene: 34495831

Centisome position: 8.28

GC content: 71.87

Gene sequence:

>942_bases
ATGCCCCGCACTCCGCTCTCCTGCCTCGCCCTGCTCGCCTGCGCCCTTCCCGCCGCCGCCGCGGACCCGTCCCGCATCGG
CATCTCGGTCGGGGCGCTGGACAACCCGTTCTACCAGGCGCTGGCGCGCGGCGCGGTGCAGGCCGCCCACCGGCTCAATC
CAGGCGTGCGCATCACGTCTCAATCGGCCAATTTCACGCTGGGGCAACAGCAGCAGCAACTGCGGCAGCTGATCGCGCAG
AAAGTGGACCTGATCCTGCTGGGCGCGGTGGACAGCAACGCGGTGGCCCCGCTGGTTCGCCAGGCCCGCGCCGCCGGCAT
CACCGTGGTGGCGGTGGACGTGGACGCGCCCGATGTCGACGGCACGGTGAAGTCCGACAACCATCTGGCCGGCGAGATCG
TCTGCCGCTACCTGGCCCAACGGCTGCGCGGCCGCGGCACGCTGATGATCCAGGGCGGGCCGCCGGTCACCTCGGTCAGC
GACCGCATCGCCGGCTGCCATGCCGCGCTGGCCGCCTTTCCCCACATCCGCGCAGTCGACGACGGCCTCAACGCCCAAGG
CTCCAGCCTGGGCGGCCAGCGCGCGATGCAGCAGGCCTTGCAGCGCTGGCCGGATATGGCCGCGGTGTTCACCATCAACG
ACCGCCAGGCGCTGGGCGTGGAGAAAGCCTTGCTCGCCGCCGGGCGAAAGCAGGTGCTGATCGGCTCGGTGGACGGCTCG
CCCGACATCGAGCGCGCGCTGCGGCTGCCCGGCCAGATCGTCGTCTCCGCCAGCCAGTCGCCGTACCTGCTGGGACGCGA
AGCGGTGAAGCTGGGCGTCAAGCTGCGCCGCGGCGACACCTCCGTCCGCCACGTGACGGTACCGGTGGGACTGGTCACCC
GCGACAACCTCTCCCGCTACCAGGGTTGGCAAGCCGCGATGCCGGGCGCCGGCCAGCCCTGA

Upstream 100 bases:

>100_bases
AGCTGGCGTGGCGCGCGCAGCGCCATCTCGAATCCAGGACGGTCGCAAGCGCGACGGGATGAGCTAGACTGATACCAGTC
AGACTCGCACCCGTCCCGCC

Downstream 100 bases:

>100_bases
CCGCGCGGGCTTTGACCTGGGCGTCCGCCGGTGTCACACTGCCGGCTCGACATCCATTACCAGATCAAAGGATTTGCCAT
GTTTGCTTCCCGCTCGATCA

Product: ribose ABC transporter substrate-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MPRTPLSCLALLACALPAAAADPSRIGISVGALDNPFYQALARGAVQAAHRLNPGVRITSQSANFTLGQQQQQLRQLIAQ
KVDLILLGAVDSNAVAPLVRQARAAGITVVAVDVDAPDVDGTVKSDNHLAGEIVCRYLAQRLRGRGTLMIQGGPPVTSVS
DRIAGCHAALAAFPHIRAVDDGLNAQGSSLGGQRAMQQALQRWPDMAAVFTINDRQALGVEKALLAAGRKQVLIGSVDGS
PDIERALRLPGQIVVSASQSPYLLGREAVKLGVKLRRGDTSVRHVTVPVGLVTRDNLSRYQGWQAAMPGAGQP

Sequences:

>Translated_313_residues
MPRTPLSCLALLACALPAAAADPSRIGISVGALDNPFYQALARGAVQAAHRLNPGVRITSQSANFTLGQQQQQLRQLIAQ
KVDLILLGAVDSNAVAPLVRQARAAGITVVAVDVDAPDVDGTVKSDNHLAGEIVCRYLAQRLRGRGTLMIQGGPPVTSVS
DRIAGCHAALAAFPHIRAVDDGLNAQGSSLGGQRAMQQALQRWPDMAAVFTINDRQALGVEKALLAAGRKQVLIGSVDGS
PDIERALRLPGQIVVSASQSPYLLGREAVKLGVKLRRGDTSVRHVTVPVGLVTRDNLSRYQGWQAAMPGAGQP
>Mature_312_residues
PRTPLSCLALLACALPAAAADPSRIGISVGALDNPFYQALARGAVQAAHRLNPGVRITSQSANFTLGQQQQQLRQLIAQK
VDLILLGAVDSNAVAPLVRQARAAGITVVAVDVDAPDVDGTVKSDNHLAGEIVCRYLAQRLRGRGTLMIQGGPPVTSVSD
RIAGCHAALAAFPHIRAVDDGLNAQGSSLGGQRAMQQALQRWPDMAAVFTINDRQALGVEKALLAAGRKQVLIGSVDGSP
DIERALRLPGQIVVSASQSPYLLGREAVKLGVKLRRGDTSVRHVTVPVGLVTRDNLSRYQGWQAAMPGAGQP

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=294, Percent_Identity=29.5918367346939, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790674, Length=217, Percent_Identity=27.1889400921659, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32848; Mature: 32717

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTPLSCLALLACALPAAAADPSRIGISVGALDNPFYQALARGAVQAAHRLNPGVRITS
CCCCHHHHHHHHHHHCCCCCCCCHHCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
QSANFTLGQQQQQLRQLIAQKVDLILLGAVDSNAVAPLVRQARAAGITVVAVDVDAPDVD
CCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCC
GTVKSDNHLAGEIVCRYLAQRLRGRGTLMIQGGPPVTSVSDRIAGCHAALAAFPHIRAVD
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECC
DGLNAQGSSLGGQRAMQQALQRWPDMAAVFTINDRQALGVEKALLAAGRKQVLIGSVDGS
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCC
PDIERALRLPGQIVVSASQSPYLLGREAVKLGVKLRRGDTSVRHVTVPVGLVTRDNLSRY
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCEEEECCCCCEEEEEEEEEEEECCCHHHH
QGWQAAMPGAGQP
CCCCCCCCCCCCC
>Mature Secondary Structure 
PRTPLSCLALLACALPAAAADPSRIGISVGALDNPFYQALARGAVQAAHRLNPGVRITS
CCCHHHHHHHHHHHCCCCCCCCHHCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
QSANFTLGQQQQQLRQLIAQKVDLILLGAVDSNAVAPLVRQARAAGITVVAVDVDAPDVD
CCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCC
GTVKSDNHLAGEIVCRYLAQRLRGRGTLMIQGGPPVTSVSDRIAGCHAALAAFPHIRAVD
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECC
DGLNAQGSSLGGQRAMQQALQRWPDMAAVFTINDRQALGVEKALLAAGRKQVLIGSVDGS
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCC
PDIERALRLPGQIVVSASQSPYLLGREAVKLGVKLRRGDTSVRHVTVPVGLVTRDNLSRY
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCEEEECCCCCEEEEEEEEEEEECCCHHHH
QGWQAAMPGAGQP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]