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The map label for this gene is gltD [H]

Identifier: 161594049

GI number: 161594049

Start: 4363336

End: 4364751

Strand: Reverse

Name: gltD [H]

Synonym: CV_4037

Alternate gene names: 161594049

Gene position: 4364751-4363336 (Counterclockwise)

Preceding gene: 34499493

Following gene: 34499491

Centisome position: 91.87

GC content: 58.69

Gene sequence:

>1416_bases
ATGTCCGATGTATTCCAGTTCATGAAGCTGTCGCGCAACCCAGGCGAAAAGGTCGAAGCGTCGGTGCGCAAGGTCGAGTT
CAAGGAAATCTACCAGCCGCTGCACGCGGTGGATGCCGCCGACCAGGCCGGCCGCTGCCTGTCCTGCGGCAATCCGTATT
GCGAGTGGGAGTGCCCGGTGCACAACTACATTCCCAACTGGCTGAAGCTGGTGGAGGAGGGGCGCCTGTTCGAGGCGGCG
GAGTTATCGCATCAGACCAACAGCCTGCCCGAGATCTGCGGCCGGGTCTGCCCGCAAGACCGGCTGTGTGAGGGGGCCTG
TACCTTGAATCAGGGGGGCTTCGGCGCAGTATCCATCGGCAGCATAGAGAAGTACATTACCGACGAGGCATTCAAGGCGG
GTTGGCGTCCCGACATGTCCAAGGTGGTTTGGACGGATAAGACTGTGGGGATCATCGGCGCTGGTCCCGCGGGCCTGGCC
TGTGCCGACGTGTTGGTGCGCAATGGGGTCAAGGCTGTGGTGTATGACCGCTATGAGGAGATCGGCGGGTTGTTGACCTT
TGGTATTCCGGAGTTCAAGTTGGAAAAAAGCATTGTCCACCGCCGCAGAGAGATACTGGAAGGAATGGGGGTGGAGTTTG
TGCTGAATACTGAGGTCGGCAAGGACATCAGCTTTGACAAGCTTATGATTAAGCACGATGCCGTGTTTATGGGTATGGGC
GCGTACAAGGCCATGCGGGGGGGCTTTCCTGGAGAAGACAGCAAGGGTGTGCTGCAGGCTTTGCCCTATCTGATTAATAA
TGTGCGCCAGTCTATGGGAACCTTGCCTGTAGACGAAGCAGCCATCTCGATGAAGGGCAAGCGAGTATTGGTGCTGGGTG
GCGGGGACACTGCGATGGACTGCAACCGCACTGCGATCCGACAAGGCGCGAAACGTGTGATCTGCGCTTACCGTCGCGAC
GAGGCCAATATGCCCGGTTCCAGACGAGAAGTCAGCAACGCGCGCGAAGAAGGGGTGGAGTTTTTGTGGAACCGGCAGCC
TTTGGCGATTGAGCCGATGATAGGCGGCACCCTGGCTGTGAAGCTGGGCGAGACTCGGCTGAGCGAGGCTGGAGCCAAGG
GGCGAAGCGCTGTTGAACTTGTGCCGGGGGGGGGGGAGGTCTTAGAATGCGACCACGTTATCATAGCGTTCGGGTTTCAA
GCCGAAGCGGCTGCTTGGTTTGACGCCTGCGGGGTACGAACTGAAAATGGGTCCGGTAGAACCAAAGTCTCCCGAGGAGG
CAAGTACCCTTACCAGACTAGCAATCCCAAAGTATTTGCCGGAGGGGATCAAGTTCGAGGCGCGGATCTTGTTGTGCGCG
CAGTGTTCGAGGGGCGTCAGGCTGCGGAGTCGTTGTGCGCGTATTTGTTTGACTAG

Upstream 100 bases:

>100_bases
GGCCGCCAAGCTGGAAAGCCTGCTCAAAGACTAAGCGCCGCTGGCAACTCAATACCTGGCGGCGCAGGAAGCGCCGCCTA
TCCCGATGGGAGAGAACAAG

Downstream 100 bases:

>100_bases
AGAAGGCGGGGGGCTAGCCTCAGCCAGGCCGCCCGGCTGGTGGTGGCTGGTCGCGGGACGACGCACGAGACGGTTTCTCA
TCAGGGAAACCCGGTCTGTC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSDVFQFMKLSRNPGEKVEASVRKVEFKEIYQPLHAVDAADQAGRCLSCGNPYCEWECPVHNYIPNWLKLVEEGRLFEAA
ELSHQTNSLPEICGRVCPQDRLCEGACTLNQGGFGAVSIGSIEKYITDEAFKAGWRPDMSKVVWTDKTVGIIGAGPAGLA
CADVLVRNGVKAVVYDRYEEIGGLLTFGIPEFKLEKSIVHRRREILEGMGVEFVLNTEVGKDISFDKLMIKHDAVFMGMG
AYKAMRGGFPGEDSKGVLQALPYLINNVRQSMGTLPVDEAAISMKGKRVLVLGGGDTAMDCNRTAIRQGAKRVICAYRRD
EANMPGSRREVSNAREEGVEFLWNRQPLAIEPMIGGTLAVKLGETRLSEAGAKGRSAVELVPGGGEVLECDHVIIAFGFQ
AEAAAWFDACGVRTENGSGRTKVSRGGKYPYQTSNPKVFAGGDQVRGADLVVRAVFEGRQAAESLCAYLFD

Sequences:

>Translated_471_residues
MSDVFQFMKLSRNPGEKVEASVRKVEFKEIYQPLHAVDAADQAGRCLSCGNPYCEWECPVHNYIPNWLKLVEEGRLFEAA
ELSHQTNSLPEICGRVCPQDRLCEGACTLNQGGFGAVSIGSIEKYITDEAFKAGWRPDMSKVVWTDKTVGIIGAGPAGLA
CADVLVRNGVKAVVYDRYEEIGGLLTFGIPEFKLEKSIVHRRREILEGMGVEFVLNTEVGKDISFDKLMIKHDAVFMGMG
AYKAMRGGFPGEDSKGVLQALPYLINNVRQSMGTLPVDEAAISMKGKRVLVLGGGDTAMDCNRTAIRQGAKRVICAYRRD
EANMPGSRREVSNAREEGVEFLWNRQPLAIEPMIGGTLAVKLGETRLSEAGAKGRSAVELVPGGGEVLECDHVIIAFGFQ
AEAAAWFDACGVRTENGSGRTKVSRGGKYPYQTSNPKVFAGGDQVRGADLVVRAVFEGRQAAESLCAYLFD
>Mature_470_residues
SDVFQFMKLSRNPGEKVEASVRKVEFKEIYQPLHAVDAADQAGRCLSCGNPYCEWECPVHNYIPNWLKLVEEGRLFEAAE
LSHQTNSLPEICGRVCPQDRLCEGACTLNQGGFGAVSIGSIEKYITDEAFKAGWRPDMSKVVWTDKTVGIIGAGPAGLAC
ADVLVRNGVKAVVYDRYEEIGGLLTFGIPEFKLEKSIVHRRREILEGMGVEFVLNTEVGKDISFDKLMIKHDAVFMGMGA
YKAMRGGFPGEDSKGVLQALPYLINNVRQSMGTLPVDEAAISMKGKRVLVLGGGDTAMDCNRTAIRQGAKRVICAYRRDE
ANMPGSRREVSNAREEGVEFLWNRQPLAIEPMIGGTLAVKLGETRLSEAGAKGRSAVELVPGGGEVLECDHVIIAFGFQA
EAAAWFDACGVRTENGSGRTKVSRGGKYPYQTSNPKVFAGGDQVRGADLVVRAVFEGRQAAESLCAYLFD

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=449, Percent_Identity=28.5077951002227, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1789606, Length=470, Percent_Identity=61.9148936170213, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1788811, Length=474, Percent_Identity=55.6962025316456, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI87082180, Length=464, Percent_Identity=53.8793103448276, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1788468, Length=446, Percent_Identity=29.8206278026906, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=30.3529411764706, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=497, Percent_Identity=35.2112676056338, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71984108, Length=469, Percent_Identity=30.2771855010661, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17543860, Length=246, Percent_Identity=23.1707317073171, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=497, Percent_Identity=33.6016096579477, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=34.7736625514403, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=34.7736625514403, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=34.7736625514403, Blast_Score=238, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=34.7736625514403, Blast_Score=238, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24640763, Length=452, Percent_Identity=27.8761061946903, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI18858217, Length=452, Percent_Identity=27.8761061946903, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51309; Mature: 51178

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVFQFMKLSRNPGEKVEASVRKVEFKEIYQPLHAVDAADQAGRCLSCGNPYCEWECPV
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEECCCCCCCEEECCH
HNYIPNWLKLVEEGRLFEAAELSHQTNSLPEICGRVCPQDRLCEGACTLNQGGFGAVSIG
HHHCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEECCCCCCCEEECC
SIEKYITDEAFKAGWRPDMSKVVWTDKTVGIIGAGPAGLACADVLVRNGVKAVVYDRYEE
HHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHCCCCEEEHHHHHH
IGGLLTFGIPEFKLEKSIVHRRREILEGMGVEFVLNTEVGKDISFDKLMIKHDAVFMGMG
HCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHEEEECCEEEEECC
AYKAMRGGFPGEDSKGVLQALPYLINNVRQSMGTLPVDEAAISMKGKRVLVLGGGDTAMD
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCCC
CNRTAIRQGAKRVICAYRRDEANMPGSRREVSNAREEGVEFLWNRQPLAIEPMIGGTLAV
CCHHHHHHCHHHEEEHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEE
KLGETRLSEAGAKGRSAVELVPGGGEVLECDHVIIAFGFQAEAAAWFDACGVRTENGSGR
EECCHHHHHCCCCCCCEEEEECCCCCEEEECEEEEEECCCHHHHHHHHHCCCEECCCCCC
TKVSRGGKYPYQTSNPKVFAGGDQVRGADLVVRAVFEGRQAAESLCAYLFD
CEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVFQFMKLSRNPGEKVEASVRKVEFKEIYQPLHAVDAADQAGRCLSCGNPYCEWECPV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEECCCCCCCEEECCH
HNYIPNWLKLVEEGRLFEAAELSHQTNSLPEICGRVCPQDRLCEGACTLNQGGFGAVSIG
HHHCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEECCCCCCCEEECC
SIEKYITDEAFKAGWRPDMSKVVWTDKTVGIIGAGPAGLACADVLVRNGVKAVVYDRYEE
HHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHCCCCEEEHHHHHH
IGGLLTFGIPEFKLEKSIVHRRREILEGMGVEFVLNTEVGKDISFDKLMIKHDAVFMGMG
HCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHEEEECCEEEEECC
AYKAMRGGFPGEDSKGVLQALPYLINNVRQSMGTLPVDEAAISMKGKRVLVLGGGDTAMD
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCCC
CNRTAIRQGAKRVICAYRRDEANMPGSRREVSNAREEGVEFLWNRQPLAIEPMIGGTLAV
CCHHHHHHCHHHEEEHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEE
KLGETRLSEAGAKGRSAVELVPGGGEVLECDHVIIAFGFQAEAAAWFDACGVRTENGSGR
EECCHHHHHCCCCCCCEEEEECCCCCEEEECEEEEEECCCHHHHHHHHHCCCEECCCCCC
TKVSRGGKYPYQTSNPKVFAGGDQVRGADLVVRAVFEGRQAAESLCAYLFD
CEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]