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The map label for this gene is purT [H]

Identifier: 33864531

GI number: 33864531

Start: 2397156

End: 2398322

Strand: Direct

Name: purT [H]

Synonym: PMT2267

Alternate gene names: 33864531

Gene position: 2397156-2398322 (Clockwise)

Preceding gene: 33864530

Following gene: 33864536

Centisome position: 99.43

GC content: 50.81

Gene sequence:

>1167_bases
ATGACTGTCTTACCAAAAACCTTGCTGTTACTTGGCAGCGGAGAACTCGGCAAAGAAATAACAATCGCCGCTCAACGGTT
GGGCTGCCATGTCATCGCCTGTGATCGATACTCCGGGGCCCCGGCCATGCAAGTAGCAGACCAAGCAGAGGTTCTGGACA
TGAACAATTCAGAAGCCCTCACAGCCATCATCAGGCATCACCAACCGGATGTAGTCATCCCAGAGATCGAAGCATTGGCA
GTCAATGCACTAGCAGAGCTTGAAAACGAAGGAATCACAGTGATCCCTACGGCCCGAGCCACAGCCATAACGATGAATCG
CGATCGAATCAGAGATCTCGCCAGCGCTGAATTGGCTCTACTGACCCCAAAATTTGCTTATGCCGGCAGTGCCGAAGAAC
TCAAACATGCAGCTGAGCCCCTGGGCTGGCCAGTGGTGGTCAAACCAGTCATGAGCTCCTCCGGCAAAGGCCAAAGTGTG
GTTAGCAATCCTGAAGGCCTACGGCAAGCCTGGCAAGCCGCTATGGCAGGGTCCCGGGGTAACTCACCGCGAGTCATCGT
GGAAGAGTTCCTTCACTTTGATCTCGAAATTACCTTGCTCACAATTCGCCAAGAGGATGGCTCCACCCTCTTCTGCGAAC
CAATCGGCCATGAACAAATTGGAGGAGATTATCAATGCAGCTGGCAACCAGCAGAACTCTCTACTGAACAACTCAAGCAA
GCCCAATCAATGGCTCTGACTATTACCAAAAATCTTGGTGGTGTGGGATTGTTCGGGGTGGAGTTCTTTCTTTGTGGCAA
TGAAGTGATTTTCTCGGAACTATCACCAAGACCACATGACACCGGACTTGTCACCCTTATCAGCCAAAACCTTAGTGAAT
TCGAACTACACCTGCGCGCAGTGCTGAACTTACCTATTCCTACTATTCAAACTGCAGATGCAGCAGCAAGCAGGGTAATC
CTGGCAAAAGACAATCTCTCATCCATTTCCTACAAAGGAGTGGAGAAAGCCCTCTCAGAAATTGACACCCAGATATTGCT
GTTCGGCAAACCCAATGCTCGACCAGGGCGTCGCATGGGGGTTGCTTTAGCAAAAGGCAAGTCTATGGAAGCAGTTCGCT
CTAAAGCTGATCGAGCCGCCGCATCCATCCAGGTAATAAAAGGGTAA

Upstream 100 bases:

>100_bases
TCCCTGATCTCAAAGTGTAAAGGGATCTCAAATACCACTTTTAAGGCAATTCCCCTAACCAGCCGAGCGCGAAGATCAAC
TCATCATCAACACCTTCATG

Downstream 100 bases:

>100_bases
GGGTGTCAGTTAAGACTTTAAGAAAAATCGATAGTGATTTAAGCCATCTAAAAGTCCAAACAAATGCGGGTTGTTAGCAA
AAAACACTCGTTGCTGATGT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MTVLPKTLLLLGSGELGKEITIAAQRLGCHVIACDRYSGAPAMQVADQAEVLDMNNSEALTAIIRHHQPDVVIPEIEALA
VNALAELENEGITVIPTARATAITMNRDRIRDLASAELALLTPKFAYAGSAEELKHAAEPLGWPVVVKPVMSSSGKGQSV
VSNPEGLRQAWQAAMAGSRGNSPRVIVEEFLHFDLEITLLTIRQEDGSTLFCEPIGHEQIGGDYQCSWQPAELSTEQLKQ
AQSMALTITKNLGGVGLFGVEFFLCGNEVIFSELSPRPHDTGLVTLISQNLSEFELHLRAVLNLPIPTIQTADAAASRVI
LAKDNLSSISYKGVEKALSEIDTQILLFGKPNARPGRRMGVALAKGKSMEAVRSKADRAAASIQVIKG

Sequences:

>Translated_388_residues
MTVLPKTLLLLGSGELGKEITIAAQRLGCHVIACDRYSGAPAMQVADQAEVLDMNNSEALTAIIRHHQPDVVIPEIEALA
VNALAELENEGITVIPTARATAITMNRDRIRDLASAELALLTPKFAYAGSAEELKHAAEPLGWPVVVKPVMSSSGKGQSV
VSNPEGLRQAWQAAMAGSRGNSPRVIVEEFLHFDLEITLLTIRQEDGSTLFCEPIGHEQIGGDYQCSWQPAELSTEQLKQ
AQSMALTITKNLGGVGLFGVEFFLCGNEVIFSELSPRPHDTGLVTLISQNLSEFELHLRAVLNLPIPTIQTADAAASRVI
LAKDNLSSISYKGVEKALSEIDTQILLFGKPNARPGRRMGVALAKGKSMEAVRSKADRAAASIQVIKG
>Mature_387_residues
TVLPKTLLLLGSGELGKEITIAAQRLGCHVIACDRYSGAPAMQVADQAEVLDMNNSEALTAIIRHHQPDVVIPEIEALAV
NALAELENEGITVIPTARATAITMNRDRIRDLASAELALLTPKFAYAGSAEELKHAAEPLGWPVVVKPVMSSSGKGQSVV
SNPEGLRQAWQAAMAGSRGNSPRVIVEEFLHFDLEITLLTIRQEDGSTLFCEPIGHEQIGGDYQCSWQPAELSTEQLKQA
QSMALTITKNLGGVGLFGVEFFLCGNEVIFSELSPRPHDTGLVTLISQNLSEFELHLRAVLNLPIPTIQTADAAASRVIL
AKDNLSSISYKGVEKALSEIDTQILLFGKPNARPGRRMGVALAKGKSMEAVRSKADRAAASIQVIKG

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=378, Percent_Identity=50.5291005291005, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1786733, Length=176, Percent_Identity=30.6818181818182, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=26.7029972752044, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 41629; Mature: 41498

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLPKTLLLLGSGELGKEITIAAQRLGCHVIACDRYSGAPAMQVADQAEVLDMNNSEAL
CCCCCCEEEEECCCCCCCEEEEEEHHHCEEEEEECCCCCCCHHHHCCCHHEECCCCCHHH
TAIIRHHQPDVVIPEIEALAVNALAELENEGITVIPTARATAITMNRDRIRDLASAELAL
HHHHHHCCCCEECCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECHHHHHHHHHCCEEE
LTPKFAYAGSAEELKHAAEPLGWPVVVKPVMSSSGKGQSVVSNPEGLRQAWQAAMAGSRG
ECCCHHCCCCHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
NSPRVIVEEFLHFDLEITLLTIRQEDGSTLFCEPIGHEQIGGDYQCSWQPAELSTEQLKQ
CCCHHHHHHHHCCCEEEEEEEEEECCCCEEEECCCCCCCCCCCEEECCCCCCCCHHHHHH
AQSMALTITKNLGGVGLFGVEFFLCGNEVIFSELSPRPHDTGLVTLISQNLSEFELHLRA
HHHEEEEEECCCCCEEEEEEEEEEECCHHEEHHCCCCCCCCCEEEEECCCHHHHHHHHHH
VLNLPIPTIQTADAAASRVILAKDNLSSISYKGVEKALSEIDTQILLFGKPNARPGRRMG
HHCCCCCCCCCCCHHHCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHE
VALAKGKSMEAVRSKADRAAASIQVIKG
EEEECCCCHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure 
TVLPKTLLLLGSGELGKEITIAAQRLGCHVIACDRYSGAPAMQVADQAEVLDMNNSEAL
CCCCCEEEEECCCCCCCEEEEEEHHHCEEEEEECCCCCCCHHHHCCCHHEECCCCCHHH
TAIIRHHQPDVVIPEIEALAVNALAELENEGITVIPTARATAITMNRDRIRDLASAELAL
HHHHHHCCCCEECCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECHHHHHHHHHCCEEE
LTPKFAYAGSAEELKHAAEPLGWPVVVKPVMSSSGKGQSVVSNPEGLRQAWQAAMAGSRG
ECCCHHCCCCHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
NSPRVIVEEFLHFDLEITLLTIRQEDGSTLFCEPIGHEQIGGDYQCSWQPAELSTEQLKQ
CCCHHHHHHHHCCCEEEEEEEEEECCCCEEEECCCCCCCCCCCEEECCCCCCCCHHHHHH
AQSMALTITKNLGGVGLFGVEFFLCGNEVIFSELSPRPHDTGLVTLISQNLSEFELHLRA
HHHEEEEEECCCCCEEEEEEEEEEECCHHEEHHCCCCCCCCCEEEEECCCHHHHHHHHHH
VLNLPIPTIQTADAAASRVILAKDNLSSISYKGVEKALSEIDTQILLFGKPNARPGRRMG
HHCCCCCCCCCCCHHHCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHE
VALAKGKSMEAVRSKADRAAASIQVIKG
EEEECCCCHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA