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The map label for this gene is argG [H]

Identifier: 33864525

GI number: 33864525

Start: 2392863

End: 2394068

Strand: Reverse

Name: argG [H]

Synonym: PMT2261

Alternate gene names: 33864525

Gene position: 2394068-2392863 (Counterclockwise)

Preceding gene: 33864526

Following gene: 33864519

Centisome position: 99.3

GC content: 51.91

Gene sequence:

>1206_bases
ATGGGGCGTGCGAAGAAGGTAGTGCTTGCTTATTCCGGTGGTGTTGATACCAGCGTTTGCATCCCATACCTCAAGCATGA
ATGGGGGGTAGAGGAGGTGATCACCTTTGCTGCCGATTTAGGTCAGGGCGATGAGCTTGATCCCATCCGCCTTAAGGCTC
TTGATGCTGGTGCTAGTCAGTCTCTGGTGGGAGATTTGATAGAGCCTTTTGTAGAACAGTTTGCTTTGCCGGCGATTCGT
GCCAATGCTCTTTACGAAGGTCGTTATCCACTGTCTACGGCTTTAGCTCGCCCCCTGATTGCTCGTCAGTTGGTAGAGGT
TGCCAGGGAGGTCGGGGCCGATGCGGTGGCTCATGGCTGTACGGGTAAAGGCAACGATCAGGTGCGTTTTGATTTAGCCA
TTGCAGCACTAGCACCTGATCTCAAGGTGCTAACTCCCGCTCGGGAATGGAGCATGAGCAGAGAGGAGGCGATCGCCTAT
GGCGAACGCTGTGGGATCCCTGCCCCCGTGAGTAAGAAATCGCCTTATTCGATAGATCTGAATCTGTTGGGTCGGAGTAT
TGAAGCCGGTCCTCTTGAAGATCTGATGGTGGCTCCGCCCGAGGAGGTCTTTGCCATGACCTCCTCGATTGACGCTGCAC
CCTCTCAGGCTCAGGACATTGAGATCAGTTTTGAAGCTGGTAATCCTGTGGCTATTGATGGGGTGCGACTTGATTCGGTG
GGTTTGATCAAGGAGGCGAATCGTCTTGCTGGACGGCATGGTTTCGGTCGCCTTGACATCATTGAGAATCGGGTGGTTGG
TATCAAGAGTCGGGAGATTTATGAAACTCCAGGGTTACTTTTATTGATTCGTGCTCATCAGGAATTGGAGAGTTTGACAC
TTGCTGCGGATGTGTTGCGCATGAAGCGTCAATTGGAGATGCAATGGGCTGAGCTGGTCTATCAGGGTCTTTGGTTTTCC
CCTTTGAAGGATGCTCTGGATGGATTTATGGATCGCACTCAGACTTATGTGAATGGCTTGGTGCGGATTCGTCTCCAAAA
AGGCAATGCCATGGTGATTGGCCGGAGCTCTGACACCAACAGCTTGTATATATCTGAGATGGCTACCTATGGCAGCGAGG
ATAACTTTGACCACAGGGCTGCTGAAGGATTTATCTACATCTGGGGACTTCCAAGTCGCCTTTGGGCAGCAGCTCGGCGA
GGTTAA

Upstream 100 bases:

>100_bases
TCGGTATTTGGGTCATGCGGGTGGGATTTTCTACGGCTGATTCCGGGTCTTTGGTCAACGGGAAGGGAGAATCTTTGAAT
TGTGATTCGGAGTCCAGTTG

Downstream 100 bases:

>100_bases
GTTGTGATCTTTTGATTAGTTTTATTGTGGAGATTGCCCTCTGCCTCCAAGCCATCTTGAGCGAAGCAGGGAAGGTACTT
TTAATGGGTGAGGCTCCTCA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MGRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITFAADLGQGDELDPIRLKALDAGASQSLVGDLIEPFVEQFALPAIR
ANALYEGRYPLSTALARPLIARQLVEVAREVGADAVAHGCTGKGNDQVRFDLAIAALAPDLKVLTPAREWSMSREEAIAY
GERCGIPAPVSKKSPYSIDLNLLGRSIEAGPLEDLMVAPPEEVFAMTSSIDAAPSQAQDIEISFEAGNPVAIDGVRLDSV
GLIKEANRLAGRHGFGRLDIIENRVVGIKSREIYETPGLLLLIRAHQELESLTLAADVLRMKRQLEMQWAELVYQGLWFS
PLKDALDGFMDRTQTYVNGLVRIRLQKGNAMVIGRSSDTNSLYISEMATYGSEDNFDHRAAEGFIYIWGLPSRLWAAARR
G

Sequences:

>Translated_401_residues
MGRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITFAADLGQGDELDPIRLKALDAGASQSLVGDLIEPFVEQFALPAIR
ANALYEGRYPLSTALARPLIARQLVEVAREVGADAVAHGCTGKGNDQVRFDLAIAALAPDLKVLTPAREWSMSREEAIAY
GERCGIPAPVSKKSPYSIDLNLLGRSIEAGPLEDLMVAPPEEVFAMTSSIDAAPSQAQDIEISFEAGNPVAIDGVRLDSV
GLIKEANRLAGRHGFGRLDIIENRVVGIKSREIYETPGLLLLIRAHQELESLTLAADVLRMKRQLEMQWAELVYQGLWFS
PLKDALDGFMDRTQTYVNGLVRIRLQKGNAMVIGRSSDTNSLYISEMATYGSEDNFDHRAAEGFIYIWGLPSRLWAAARR
G
>Mature_400_residues
GRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITFAADLGQGDELDPIRLKALDAGASQSLVGDLIEPFVEQFALPAIRA
NALYEGRYPLSTALARPLIARQLVEVAREVGADAVAHGCTGKGNDQVRFDLAIAALAPDLKVLTPAREWSMSREEAIAYG
ERCGIPAPVSKKSPYSIDLNLLGRSIEAGPLEDLMVAPPEEVFAMTSSIDAAPSQAQDIEISFEAGNPVAIDGVRLDSVG
LIKEANRLAGRHGFGRLDIIENRVVGIKSREIYETPGLLLLIRAHQELESLTLAADVLRMKRQLEMQWAELVYQGLWFSP
LKDALDGFMDRTQTYVNGLVRIRLQKGNAMVIGRSSDTNSLYISEMATYGSEDNFDHRAAEGFIYIWGLPSRLWAAARRG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=403, Percent_Identity=41.1910669975186, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI16950633, Length=403, Percent_Identity=41.1910669975186, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1789563, Length=384, Percent_Identity=30.2083333333333, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6324514, Length=410, Percent_Identity=37.8048780487805, Blast_Score=266, Evalue=3e-72,
Organism=Drosophila melanogaster, GI21358151, Length=394, Percent_Identity=39.3401015228426, Blast_Score=262, Evalue=3e-70,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 43825; Mature: 43693

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITFAADLGQGDELDPIRLKALDAGASQ
CCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCH
SLVGDLIEPFVEQFALPAIRANALYEGRYPLSTALARPLIARQLVEVAREVGADAVAHGC
HHHHHHHHHHHHHHCCCHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TGKGNDQVRFDLAIAALAPDLKVLTPAREWSMSREEAIAYGERCGIPAPVSKKSPYSIDL
CCCCCCEEEEEEEEEHHCCCCEEECCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEH
NLLGRSIEAGPLEDLMVAPPEEVFAMTSSIDAAPSQAQDIEISFEAGNPVAIDGVRLDSV
EECCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECEEECCH
GLIKEANRLAGRHGFGRLDIIENRVVGIKSREIYETPGLLLLIRAHQELESLTLAADVLR
HHHHHHHHHHCCCCCCCEEEECCCEEECCCCCEECCCCEEEEEECHHHHHHHHHHHHHHH
MKRQLEMQWAELVYQGLWFSPLKDALDGFMDRTQTYVNGLVRIRLQKGNAMVIGRSSDTN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEECCCEEEEECCCCCC
SLYISEMATYGSEDNFDHRAAEGFIYIWGLPSRLWAAARRG
CEEEEHHHHCCCCCCCCCHHHCCEEEEEECHHHHHHHHCCC
>Mature Secondary Structure 
GRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITFAADLGQGDELDPIRLKALDAGASQ
CCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCH
SLVGDLIEPFVEQFALPAIRANALYEGRYPLSTALARPLIARQLVEVAREVGADAVAHGC
HHHHHHHHHHHHHHCCCHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TGKGNDQVRFDLAIAALAPDLKVLTPAREWSMSREEAIAYGERCGIPAPVSKKSPYSIDL
CCCCCCEEEEEEEEEHHCCCCEEECCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEH
NLLGRSIEAGPLEDLMVAPPEEVFAMTSSIDAAPSQAQDIEISFEAGNPVAIDGVRLDSV
EECCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECEEECCH
GLIKEANRLAGRHGFGRLDIIENRVVGIKSREIYETPGLLLLIRAHQELESLTLAADVLR
HHHHHHHHHHCCCCCCCEEEECCCEEECCCCCEECCCCEEEEEECHHHHHHHHHHHHHHH
MKRQLEMQWAELVYQGLWFSPLKDALDGFMDRTQTYVNGLVRIRLQKGNAMVIGRSSDTN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEECCCEEEEECCCCCC
SLYISEMATYGSEDNFDHRAAEGFIYIWGLPSRLWAAARRG
CEEEEHHHHCCCCCCCCCHHHCCEEEEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA